Previous Projects

Completed projects over the past five years.

International

MuTaLig – Viac-cieľový model pre inovatívnu identifikáciu látok v procese objavovania liečiv
Multi-target paradigm for innovative ligand identification in the drug discovery process
Program: COST
Project leader: RNDr. Májeková Magdaléna PhD.
Annotation: The aim of this COST Action is to join highly-qualified research teams working in disciplines around the field of medicinal chemistry, into a novel network devoted to the multi-target issue in drug discovery. The choice of this theme is related to its marked multidisciplinary character, which can ensure a strong interaction among all COST Action participants. Currently, an important and emerging issue in modern drug discovery is to design novel or identify existing bioactive compounds, endowed with the capability to interact selectively with two or more macromolecular targets, exerting their effects against certain therapeutic goals in a synergic fashion. This leading concept stimulated this COST Action focusing on novel ligands able to recognize selected multiple targets, to promote closer scientific links among European research groups involved in medicinal chemistry field at both academic and industrial level. The research competencies of the network will span around medicinal chemistry, from synthetic chemistry, natural products and biophysics to theoretical chemistry, molecular modelling and biological screening.
Project web page: http://www.mutalig.eu/
Duration: 4.12.2015 – 29.10.2019
GLUCOLIPOTOX – Ovplyvnenie molekulovych dráh glucolipotoxicity novým karboxymetylovaným merkaptotriazinoindolovým ihibítorom aldo-ketoreduktázy AKRlBl v diabete, zápale a vekom podmienenej neurodegeneráci
Targeting Molecular Pathways of Glucolipotoxicity by a Novel Carboxymethylated Mercaptotriazinoindole Inhibitor of Aldo-Keto Reductase AKR1B1 In Diabetes, Inflammation and Age-related Neurodegeneration
Program: Bilateral – other
Project leader: Ing. Štefek Milan CSc.
Duration: 1.5.2016 – 30.4.2019
Podnetné organické syntézy inšpirované prírodou: od chémie prírodných látok poobjav liečiv
Challenging organic synthesis inspired by nature – from natural products to drug discovery.
Program: COST
Project leader: RNDr. Horáková Ľubica PhD.
Annotation: Natural products (NP) have had a major impact on chemistry, chemical biology and drug discovery and have been part of medical remedies since ancient times. Nowadays, NP represent a unique source of leads for medicinal chemistry and drugs derived from NP have found widespread use for the treatment of cancer, cardiovascular diseases, bacterial and fungal infections. The general aim of this COST Action is to advance the field and to maintain the high level of expertise in NP chemistry within Europe by combining synthetic chemistry, computational chemistry, chemical biology, and pharmacology to find new lead structures of pharmaceutical relevance. Since chemistry plays a key role in addressing the industrial requirements for preclinical candidates in terms of physicochemical properties of NP and their analogues, this Action further aims to promote the translation between fundamental academic research and industrial drug discovery by means of NP chemistry.
Duration: 15.3.2015 – 14.3.2019
Dohoda o spolupráci medzi ÚNPF SAV a Ústavom základného psychologického výskumu a výskumných metód Fakulty psychológie Univerzity Viedeň
Program: Inter-institute agreement
Project leader: MUDr. Riečanský Igor PhD.
Annotation:
Duration: 1.4.2015 – 31.12.2017
Evaluation of Quercetin and Green Tea in combination with Methotrexate for arthritis therapy (Acronym: PhytoArt 2.0)
Evaluation of Quercetin and Green Tea in combination with Methotrexate for arthritis therapy (Acronym: PhytoArt 2.0)
Program: Bilateral – other
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Duration: 1.1.2016 – 31.12.2017
Mechanizmy poškodenia srdca radiáciou a možnosti medikamentóznej prevencie.
Mechanisms of radiation injury to the heart. Preventive drug treatment.
Program: Multilateral – other
Project leader: D.h.c., Prof., MUDr. Slezák Ján DrSc., FIACS
Duration: 1.1.2014 – 31.12.2017
Multidisciplinárna analýza kombinovaného vplyvu tyreoidnych hormonov a n-3 polynenasytených mastných kyselin u potkanov
Multidisciplinary analysis of the combined effects of thyroid hormones and n-3 polyunsaturated fatty acids in rats
Program: Inter-academic agreement
Project leader: RNDr. Tribulová Narcisa DrSc.
Annotation: Thyroid hormones (TH) play a crucial role in healthy organisms, but profound alterations of their levels can affect metabolic and signaling processes in different tissues. We will use hyper- and hypothyroid rats as “model of a diseased organism” and analyze whether and how n-3 PUFA long term administration will ameliorate TH-induced pathophysiological changes. We will investigate skeletal and cardiac muscle tissue remodeling, changes in serum lipids and in body fat distribution, key enzymes of TH metabolism, oxidative stress in the whole organism and within the cell. We will focus on myosin heavy chain composition, distribution and phosphorylation of connexin-43, cellular calcium handling, membrane anisotropy, mitochondrial functions and related signaling pathways including cell death and antioxidant defense. As n-3 PUFA possess multiple sites of potential action, we believe that our complex research will contribute to a better understanding of molecular mechanisms governing n-3 PUFA actions and to their more effective application in the prevention of pathological symptoms in man.
Duration: 1.1.2015 – 31.12.2017
Odpovede krvného tlaku indukované stresom: endotelové faktory vs. centrálna regulácia sympatikového tonusu
Stress-induced pressor responses: endothelial factors vs. central regulation of sympathetic tone
Program: Inter-academic agreement
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation: Acute stress is considered to be a factor that may participate in stroke and heart attack. Mechanisms involved may be associated with inadequate acute stress-induced elevation of blood pressure (BP). On the basis of our previous joint research the aim of this project is to reveal differences in mechanisms involved in acute stress-induced pressor responses in adult normotensive and spontaneously hypertensive (SHR) rats. We will determine the role of nitric oxide (NO) in central regulation of sympathetic tone as well as the role of endothelium-derived factors (EDFs) such as NO, superoxide and hydrogen sulfide, in modulation of stress-induced BP responses. Acute stress will be induced by air-jet to the face of the rat. The effect of particular BP regulation systems will be investigated using inhibitors specific for the given system such as pentolinium (SNS), tempol (ROS), selective inhibitors of nNOS, iNOS and eNOS and other substances to inhibit selected EDFs. Resolving differences in acute stress-induced pressor responses could provide mechanism for selective prevention of dangerous stress-induced BP increase in hypertensive subjects and thus in prevention of stress-provoked stroke and heart attack.
Duration: 1.1.2016 – 31.12.2017
Sensorimotor – Stabilita postoja a trupu vo vzťahu k respiračným funkciám u zdravých jedincov a po transplantácii pľúc
Postural and core stability in association with respiratory functions in healthy and lung transplant individuals
Program: Bilateral – other
Project leader: Ing. Hlavačka František CSc.
Annotation: Lung transplantation has been shown to be effective as a treatment for fatal and severecardiopulmonary diseases. In addition to survival, one goal of performing lung transplantation isimprovement in functioning and health. Several factors contribute to the maximal work capacityachievable after transplantation. Before surgery, patients are usually debilitated from the primarydisease and long-term inactivity, and the unavoidable complications of allograft transplantation,mainly lung resection, and toxic effects of immunosuppressant therapy contribute to the reducedfunctional capacity. While numerous investigators have described the features of the respiratoryand skeletal muscle function before and after surgery, little information is currently availableregarding the functional outcomes, such as strength and power, speed of step initiation, posturaland core stability, and so forth. It is mainly due to a lack of a standard evaluation system of theseabilities. Recently, we have developed new methodology for assessment of postural sway andbreathing movements of the chest using wireless accelerometers. Yet, it was not tested in lungtransplant patients and no investigations related to the relationship of variables of neuromuscularand cardiorespiratory functions were carried out. Therefore, we will assess postural and corestability, muscle strength and cardiorespiratory functions using posturography, surface EMG,accelerometry and clinical tests in healthy young and elderly individuals and lung transplantpatients. The findings will serve as a basis for better understanding of association betweenrelated variables in these people. As a result of the project will be elaborated methodology fortesting of neuromuscular and cardiorespiratory functions using novel techniques specificallydesigned for target population. Such objective and less time-consuming assessment can be wellfitted in an out-patients department and complement so existing diagnostic methods.
Duration: 1.1.2016 – 31.12.2017
Vplyv patologických stavov na odolnosť srdca voči ischémii myokardu: štúdium molekulárnych mechanizmov a nových možností kardioprotekcie
Effect of pathological states on cardiac resistance against myocardial ischemia: study of molecular mechanisms and novel approaches to cardioprotection
Program: Inter-academic agreement
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Previously, we showed a negative impact of civilization diseases (hypertension, metabolic disorders) on adaptive mechanisms in the heart and its survival under conditions of acute ischemia/reperfusion (I/R). Comorbidities and age- and gender-related changes blunt protective cell signaling of ischemic preconditioning (PC) leading to acceleration of necrotic and apoptotic processes, impaired function of mitochondria and energy production. The project is focused on the study of molecular mechanisms that alter innate cardioprotection in the diseased myocardium and on possibilities to reactivate its adaptive potential. We will explore effects of novel forms of PC, so called „remote“ PC (induced by limb ischemia) that are less technically demanding and can be used in clinical practice, e.g., in elder patients with acute myocardial infarction. Investigation of mechanisms of „remote“ PC will enable its pharmacological simulation, e.g., using pleiotropic (other than primary) effects of PPAR agonists or their combination with PC that can facilitate stimulatory effect on cardiac resistance against I/R.
Duration: 1.1.2015 – 31.12.2017
BIOMAMI – Horčíkové nanokompozity pre biodegradovateľné medicínske implantáty
Magnesium Nanocomposites for Biodegradable Medical Implants
Program: Inter-academic agreement
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.11.2014 – 31.10.2017
An integrated European platform for pancreas cancer research: from basic science to clinical and public interventions for a rare disease
An integrated European platform for pancreas cancer research: from basic science to clinical and public interventions for a rare disease
Program: COST
Project leader: Ing. Ďurišová Mária DrSc.
Duration: 14.12.2012 – 13.12.2016
COST BM1204 – COST BM1204 : Integrovaná európska platforma pre výskum rakoviny pankreasu: od základného výskumu ku opatreniam v klinickej medicíne a verejnom zdravotníctve v oblasti zriedkavých chorôb
COST BM1204 : An integrated European platform for pancreas cancer research: from basic science to clinical and public health interventions for a rare disease
Program: COST
Project leader: RNDr. Májeková Magdaléna PhD.
Annotation: To present the method based on molecular modeling, which is connected with potential molecular target for pancreas cancer detection, treatment and prevention. The aldo-keto reductases (AKRs) are enzymes with beneficial physiological functions, but they can play also a negative role by various pathologic processes. Our aim is to elaborate models of AKR subtypes specific for pancreatic cancer, drug design and characterization of novel aldo-keto reductase inhibitors of indole type with potential therapeutic effect in relation to pancreatic cancer and to evaluate their use together with biochemical experiments.
Project web page: http://eupancreas.com
Duration: 16.7.2012 – 13.12.2016
EU-ROS – EU-ROS: Európska sieť pre výskum oxidačného stresu a redox biológiu
EU-ROS: The European Network on Oxidative Stress and Redox Biology Research
Program: COST
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.6.2013 – 31.5.2016
Phytochemicals in ameliorating rheumatoid arthritis therapy: from preclinical studies to clinical applications
Program: Bilateral – other
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Duration: 1.1.2013 – 31.12.2015
Role of the systemic inflammatory processes in the development of oxidative stress in the brain of arthritic subjects. Evaluation of experimental therapy based on new carnosine preparations
Role of the systemic inflammatory processes in the development of oxidative stress in the brain of arthritic subjects. Evaluation of experimental therapy based on new carnosine preparations
Program: Bilateral – other
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Duration: 1.1.2013 – 31.12.2015
Senzorická súhra pri postoji a chôdzi zdravých ľudí a neurologických pacientov.
Sensory Integration for stance and gait in healthy people and neurological patients.
Program: Inter-institute agreement
Project leader: Ing. Hlavačka František CSc.
Duration: 1.2.2013 – 31.12.2015
Štúdium bunkových a molekulárnych mechanizmov zapojených do kardioprotektívnych účinkov červeného palmového oleja.
Study of cellular and molecular mechamisms involved in cardioprotective effects of red palm oil.
Program: Bilateral – other
Project leader: RNDr. Tribulová Narcisa DrSc.
Duration: 1.1.2010 – 31.12.2015
KLINIPREC – Štúdium klinicky využiteľných nových foriem preconditioningu ako alternatívnej formy ochrany myokardu pred akútnou ischémiou v organizme zaťaženom civilizačnými ochoreniami
Study of clinically applicable novel forms of preconditioning as an alternative method of cardiac protection against acute ischemia in the organism challenged with civilization diseases
Program: Inter-governmental agreement
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: In the previous studies, we have found that so called civilization diseases (hypertension, metabolic disorders) have a negative impact on the adaptive mechanisms of the heart’s own resistance against ischemia and, therefore, can alter heart survival under conditions of ischemia/reperfusion (I/R). Coexistence of several pathologies, as well as the changes related to age and gender differences, suppress cell signaling involved in protective effects of classical ischemic preconditioning (PC). The latter leads to acceleration of necrotic and apoptotic processes in cardiomyocytes during I/R, reduces energy production and promotes enhanced arrhythmogenesis. The project is focused on the study of the possibilities to modulate the intensity and/or forms of adaptive stimuli with the aim to reactivate adaptive potential in pathologically altered (remodelled) myocardium as an alternative approach to cardiac protection against acute ischemia. We will explore acute and delayed effects of novel forms of PC, so called „remote“ PC (induced by ischemia of other organ, e.g., limb) that are less technically demanding and can be used in the clinical situations, in particular, in patients with acute myocardial infarction indicated for revascularization interventions or cardiosurgery. Investigation of molecular mechanisms of „remote“ PC (including its effects on the mitochondrial function, regulation of energy metabolism and cell death processes) will enable its simulation by pharmacological means alone or use their combination with PC that can strengthen the overall stimulatory effect on the myocardial resistance against subsequent ischemia.
Duration: 1.1.2015 – 31.12.2015
Ochrana srdca pr – Štúdium ochrany srdcového svalu proti poškodeniu a malígnym poruchám rytmu vyvolaným zmeneným tyroidnym stavom.
Investigation of the cardioprotection against injury and malignant arrhythmias induced by altered thyroid status.
Program: Inter-governmental agreement
Project leader: RNDr. Tribulová Narcisa DrSc.
Duration: 1.1.2014 – 31.12.2015
The characterization and functional effects of quercetin and its derivative CHNQ, a potent aldo keto reductase inhibitor, in colorectal cancer
The characterization and functional effects of quercetin and its derivative CHNQ, a potent aldo keto reductase inhibitor, in colorectal cancer
Program: Bilateral – other
Project leader: Ing. Štefek Milan CSc.
Duration: 1.1.2013 – 31.12.2015
Účinok antioxidačných látok na metabolický syndróm: rovnováha v produkcii reaktívnych foriem kyslíka a oxidu dusnatého
Effects of antioxidants on metabolic syndrome: reactive oxygen species/nitric oxide balance
Program: Inter-institute agreement
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2007 – 31.12.2015
Úloha vnútrobunkových signalizačných mechanizmov a aktivácie nukleárnych transkripčných faktorov pri adaptácii srdca na ischemicko/reperfúzne poškodenie
Intracellular signaling pathways and nuclear transcription factor activation in cardiac adaptation to ischemia/reperfusion injury
Program: Bilateral – other
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Peroxisome proliferator-activated receptors (PPAR), ligand-activated transcription factors, belong to the nuclear hormone receptor superfamily regulating expression of genes involved in different aspects of lipid metabolism, energy production and inflammation. PPAR-alpha isoform has been recognized as the central regulator of lipid catabolism, whereas PPAR-gamma regulates lipid anabolism or storage. Until recently, the function of PPAR-beta/delta isoform was relatively less explored. In the normal adult myocardium, activation of PPAR-alpha promotes mitochondrial fatty acids oxidation as the primary pathway of ATP generation. Under different physiological and pathological conditions, PPAR-alpha modulates expression of genes that determine myocardial substrate switch from fatty acids to glucose aimed at the maintenance of energy production to preserve basic cardiac function. However, the role of PPARs in the pathogenesis of a variety of heart disorders including myocardial ischemia still remains unclear. Although PPAR-alpha and PPAR-gamma synthetic agonists, hypolipidemic and antidiabetic drugs, respectively, have been reported to protect the heart against ischemia/reperfusion injury, it is still a matter of debate whether PPAR activation plays a beneficial or detrimental role in myocardial response to ischemia, in particular, in pathological conditions. The project is focused on the study of the role of PPAR modulation in the mechanisms of cardiac response to ischemia in normal and diseased heart. Specifically, it will address the role of PPAR-beta in susceptibility to ischemia in the diabetic myocardium. Finally, the involvement of PPARs in the mechanisms of pleiotropic (lipid-independent) cardioprotective effects of some hypolipidemic and antidiabetic drugs, as well as in the cellular mechanisms of endogenous cardioprotection will be also investigated. Anticipated results will contribute to identification of PPARs as a novel therapeutical target in treatment of heart diseases
Duration: 1.1.2011 – 31.12.2015
GLIADIN – Výskum a vývoj špecifických protilátok voči rôznym typom gliadínových fragmentov a štúdium vplyvu potravy a imunizácie na afinitu týchto protilátok
Program: Bilateral – other
Project leader: RNDr. Gajdošíková Alena
Annotation:
Duration: 1.1.2012 – 31.12.2015
COST CM1103 Štrukturálne podmienené navrhovanie liečiv na diagnózu a liečenie neurologických ochorení
COST CM1103 Structure-based drug design for diagnosis and treatment of neurological diseases
Program: COST
Project leader: RNDr. Májeková Magdaléna PhD.
Duration: 28.11.2011 – 27.11.2015
Plynné transmitery: od základného výskumu po terapeutické aplikácie
Gasotransmitters: from basic science to therapeutic applications
Program: COST
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.5.2011 – 1.5.2015
Nové hybridné mo – Biokatalytická príprava nových hybridných molekúl so zvýšenýmí kardioprotektívnymi účinkami: Cielená ochrana Na+,K+-ATPázy
Biocatalytic development of novel hybrid molecules with improved cardioprotective properties: Targeting the Na+,K+-ATPase
Program: Inter-governmental agreement
Project leader: RNDr. Vrbjar Norbert CSc.
Annotation: The aim of the project is to generate novel hybrid molecules, which will be able to control hypertension and prevent hypertension-induced cardiovascular damage. To achieve this, hybrid molecules will be designed to retain the ability to block the angiotensin II effect, protect the Na,K-ATPase against hypertension and adopt increased antioxidant ability. Oxidative stress is a well-known mechanism that is responsible for the development of vascular damage. Although it is believed that lowering blood pressure is the main protective mechanism of antihypertensive treatments, treatments that interfere with oxidative stress could be useful tools for management of these individuals. The Na+,K+-ATPase is a housekeeping enzyme that energizes the membrane potential in almost all animal cells, and it is very sensitive to pressure overload in various experimental models of hypertension. Previous studies of the Slovakian research group involved in the proposed project documented the partial protective effect of natural flavonoids against hypertension-induced deteriorations of the Na,K-ATPase in the heart and kidney. In the framework of the project the complementary experience of the Greek group in the isolation of natural products, biocatalytic preparation of conjugated molecules and oxidative stress as of the Slovak groups in the biochemical evaluation of the generated compounds and their effects in various pathological situations could result in the development of bioactive compounds that will provide enhanced protection of the Na,K-ATPase against hypertension combining the positive effect of antihypertensive molecules and natural antioxidants.
Duration: 1.1.2013 – 31.12.2014
Štúdium endogénnych kardioprotektívnych mechanizmov pri ochrane myokardu voči ischémii
Study of endogenous cardioprotective mechanisms against myocardial ischemia
Program: Inter-academic agreement
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation:
Duration: 1.1.2009 – 31.12.2014
Štúdium účinkov omega-3 mastných kyselin a palmového oleja u potkanov so zmeneným tyroidným stavom.
Investigation of the effects of omega-3 fatty acids and red palm oil in rats with altered thyroid status
Program: Inter-academic agreement
Project leader: RNDr. Tribulová Narcisa DrSc.
Duration: 1.1.2012 – 31.12.2014

National

Interakcia nitrergickej, neurotrofickej a endokrinnej signalizácie v etiopatogenéze schizofrénie
Interaction of nitrergic, neurotrophic and endocrine signaling in the etiopathogenesis of schizophrenia
Program: SRDA
Project leader: MUDr. Riečanský Igor PhD.
Annotation: Schizophrenia is a common and severe mental disorder but its pathogenesis is yet poorly understood. Susceptibility to schizophrenia is largely genetic but the genetic predisposition is determined in a complex network of interactions between multiple risk genes and environmental factors, resulting in disordered brain development and function. There is increasing evidence that chronic stress and dysregulation of several signaling pathways plays role in the neurodevelopmental impairment underlying schizophrenia. In this project, by adopting a multidisciplinary approach, we will address at several levels (genetic, neurobiological and behavioral) a candidate pathophysiological pathway, involving nitrergic, neurotrophic and stress signaling, which might be importantly involved in the disordered brain development in schizophrenia. A focus on schizophrenia endophenotypes will enable us to integrate findings from human subjects at genetic risk of the disorder with those from a rodent neurodevelopmental model of schizophrenia. This project will bring important new knowledge on the etiopathogenesis of this devastating disorder and potential novel strategies of its treatment.
Duration: 1.7.2015 – 30.6.2019
Návrh a implementácia metodiky pre rehabilitáciu pacientov s bolesťami chrbta s využitím zrakového biofeedbacku
Design and implementation of visual biofeedback for the rehabilitation of mobility deficiencies in patients with low back pain
Program: SRDA
Project leader: Ing. Hlavačka František CSc.
Annotation: The main goal of this project is to design, optimize and implement a specialized method for the improved rehabilitation of mobility deficiencies in patients suffering from low back pain (LBP). The system will be equipped with accurate inertial measuring units embedded with highly sensitive micro-electro-mechanical (MEMS) accelerometers and gyroscopes, as well as a force platform providing input signals that will be processed and displayed back to the patient (visual biofeedback). LBP is a worldwide health problem affecting people of all ages, with recurring symptoms. Based on positive long-term experiences with visual biofeedback, the project strives to design an effective rehabilitation program for the improvement of impaired trunk mobility during sitting, and also impaired balance control during stance in LBP patients. The project not only includes the accurate acquisition of postural data, but also the development of software which will process, interpret and display this data in an easy to understand way. The software is intended to be easily operated and include training tasks that can be personalized to patient specific requirements. The ultimate goal of the project is to implement complex sensor systems capable of accurate measurements but process the data into an easy to use and easy to interpret rehabilitation tool for improving postural and motor function deficiencies in patients suffering from LBP.
Duration: 1.7.2017 – 30.6.2019
NANOSIMKA – Účinok nanoenkapsulovaného simvastatínu na kardiovaskulárny systém pri experimentálnom metabolickom syndróme
Effects of nanoencapsulated simvastatin on cardiovascular system in experimental metabolic syndrome
Program: SRDA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.7.2015 – 30.6.2019
MVTS: Podnetné organické syntézy inšpirované prírodou – od chémie prírodných produktov po objav liečiv
Program: Other projects
Project leader: RNDr. Horáková Ľubica PhD.
Duration: 1.3.2015 – 31.3.2019
Efekt lyofilizátu Cornus mas L. na kardiometabolické a zápalové parametre pri experimentálnom metabolickom syndróme
Effect of lyophylisate Cornus mas L. on cardiometabolic and inflammatory parameters in experimental metabolic syndrome
Program: VEGA
Project leader: Doc.MUDr. Lietava Ján CSc.
Duration: 1.1.2016 – 31.12.2018
Inhibícia proliferácie a indukcia apoptózy v rakovinových bunkách ovplyvnením ich metabolického profilu
Inhibition of proliferation and induction of apoptosis in cancer cells by affecting the metabolic profile
Program: VEGA
Project leader: RNDr. Blaškovič Dušan PhD.
Annotation: The aim of the project is to inhibit the growth and induce apoptosis in cancer cells by affecting the following metabolic pathways: glycolysis, Krebs cycle, the pentose phosphate cycle and fatty acid synthesis. Cancer cells HeLa ( cervix ) , HCT – 116 ( colon ) and MCF – 7 ( breast ) will be incubated with inhibitors of particular metabolic pathways to determine the pathway, which inhibition caused the greatest decrease of the growth, respectively the induction of apoptosis . The project is also aimed on the study of the effect of inhibiting of various metabolic pathways by natural substances, which are non-toxic for the normal cells. Multiple pathways will be simultaneously inhibited in cancer cells using natural substances to reach the most significant suppression of cancer cell growth and/or induction of apoptosis. Research will also focus on determation of the impact of coltsfoot extract on selected metabolic pathways and viability of cancer cells.
Duration: 1.1.2015 – 31.12.2018
Mechanizmy, skorá detekcia a terapia asfyktického poškodenia v perinatálnom období – porovnanie experimentálnych údajov s klinickým obrazom asfyktického novorodenca
Mechanisms, early detection and therapy of asphyxial injury in perinatal period – comparison of experimental data with clinical observation of asphyxial newborns
Program: VEGA
Project leader: Doc. RNDr. Ujházy Eduard CSc.
Annotation: The results from our studies concerning uncovering the mechanisms and possibilities of early detection of embryo-fetal damage confirmed that our proposed model of subchronic perinatal asphyxia using rat model is suitable for next studies. We will use this model for further expansion of knowledge about the mechanisms of actions of asphyxia in the process of late organogenesis and perinatal period. It is important to detect suitable biochemical and morphological markers to reduce the risk of complications due to asphyxia during development, as well as the possibility of its early therapy via antioxidant active ingredients of natural and synthetic origin. The ability of these compounds to bind to transport blood components (albumin, hemoglobin) will be monitored and a method for testing the effects on neuronal cell cultures will be developed. Integral part of this project will be correlations of given data from experimental studies with clinical observations in full-term asphyxiated newborns.
Duration: 1.1.2015 – 31.12.2018
Názov projektu: Výskum ovplyvnenia zápalu, chronickej autoimunitnej reakcie a redoxnej regulácie organizmu v experimentálnej artritíde použitím nových látok pre adjuvantnú terapiu reumatoidnej artritídy
Program: VEGA
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Duration: 1.1.2015 – 31.12.2018
Ochrana srdca pred maladaptívnou remodeláciou extracelularnej matrix a skúmanie mechanizmov jej regresie.
Protection of the heart from maladaptive extracellular matrix remodeling and searching the mechanisms of its regression.
Program: VEGA
Project leader: RNDr. Szeiffová Bačová Barbara PhD.
Annotation: Heart diseases are accompanied by exracellular matrix remodeling and fibrosis resulting in development of heartfailure and malignant arrhythmias. Fibrosis is a major medical problem without existing cure. However, there arecardioprotective compounds that exhibit antifibrotic effects but underlying mechanisms are poorly understood.This project is aimed to characterize key factors implicated in profibrotic signaling in rats suffering fromhypertension, diabetes, hypothyroidism and post-infarction injury and to determine targets of examinedpharmacological and nonpharmacological compounds. This approach should reveal signaling pathways andfactors, whose modulation could hamper or reverse fibrosis. It is expected that findings of this preclinical studymay outline design of clinical trials how to protect the heart from its dysfunction by non-invasive way.
Duration: 1.1.2015 – 31.12.2018
Protekcia hypertenzného a zlyhávajúceho srdca blokátorom I(f) kanálu ivabradínom: porovnanie s ACE-inhibíciou a melatonímom
Protection of hypertensive and failure heart by I(f) channel blocker ivabradin: comparison with ACE inhibition and melatonin
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2015 – 31.12.2018
Protektívne účinky prírodných a syntetických látok pred oxidačným poškodením vysokomolekulového hyalurónanu, izolovaných živočíšnych buniek a ich mitochondrií
Protective effects of natural and synthetic substances against oxidative damage of high-molar-mass hyaluronan, isolated mammal cells and their mitochondria
Program: VEGA
Project leader: RNDr. Valachová Katarína PhD.
Annotation: Hyaluronan (HA) is a polysaccharide of molar mass several megaDaltons present in tissues of vertebrates. In inflammatory diseases the average molar mass of HA decreases by action of oxidants. A significant decrease of synovial fluid (SF) viscoelasticity was detected, thereby its lubricating properties are worsened. High-molar-mass HA solutions are relevantly used for in vitro studies of free radicals and oxidants performance and also for a study of protective effects of antioxidants/drugs in a role of prevention or chain-breaking degradation of HA macromolecules.Substances of synthetic origin such as antiinflammatory drugs, mitochondria targeted antioxidants and natural substances with demonstrated antioxidative effects against HA degradation will be tested as protectors of oxidative degradation of cell lines of fibroblasts NIH-3T3, B-HNF-1, VH10 and cell organels, especially mitochondria.
Duration: 1.1.2015 – 31.12.2018
Protektívny účinok NO a CO donorov pri experimentálnom infarkte myokardu s hypertenzívnymi komplikáciami
Protective effect of NO and CO donors in experimental myocardial infarction with hypertensive complications
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2015 – 31.12.2018
Redoxná regulácia profesionálnych fagocytov v krvi a v centrálnom nervovom systéme: Molekulárne mechanizmy a funkčný význam.
Redox control of the professional phagocytes in blood and in the central nervous system: Molecular mechanisms and functional significance.
Program: VEGA
Project leader: PharmDr. Jančinová Viera PhD.
Annotation: The submitted project is a continuation of our previous research in the pharmacological regulation of inflammatory processes. The focus is on neutrophils and microglial cells (resident brain macrophages) which are considered active participants in the initiation and progression of pathological states connected with chronic inflammation such as arthritis or neurodegenerative diseases. The aim of the project is to explain cellular and molecularmechanisms involved in the pharmacological modulation of these cells. Attention will be paid to drugs and derivatives of natural substances which are able to influence the production of reactive oxygen and nitrogen species. The coordinated research of two types of phagocytes creates an opportunity for the sharing of modern instruments and excellent methods as immunofluorescence microscopy or flow cytometry. Cooperation betweenbasic research and clinical practice will allow the use of these methods in the analysis of phagocytes from patients with rheumatoid arthritis.
Duration: 1.1.2016 – 31.12.2018
Rizikové faktory kardiovaskulárnych a cerebrovaskulárnych ochorení a farmakologické možnosti ich ovplyvnenia
Risk factors of cardiovascular and cerebrovascular diseases and pharmacological possibilities of their influence
Program: VEGA
Project leader: RNDr. Gáspárová Zdenka PhD.
Annotation: The project is aimed at the study of mechanisms of etiopathogenesis of metabolic syndrome (MS) and metabolic cognitive syndrome (MSC). The goal is to design new effective therapy affecting the origin and development of risk factors of cardiovascular and cerebrovascular diseases. Experiments will be performed on a genetic model of rats with hereditary hypertriglyceridemia, fed with hyperlipidemic diet. The induced metabolic disorders willbecome manifest by hypertension, hypercholesterolemia, hyperglycemia, hypertriglyceridemia, inflammatory reactions and increased markers of oxidative stress. Effect on the risk factors of MS and MCS, lipidic profile, on markers of damage at cellular and molecular level, function, behavioral and histopathological changes will be studied after repeated administration of the new pyridoindole SMe1EC2, flavonoid rutin, and atorvastatin as a standard. Action on several risk factors of cardiovascular and cerebrovascular diseases simultaneously is expected.
Duration: 1.1.2015 – 31.12.2018
Štúdium dôsledkov materskej depresie a podávania antidepresíva venlafaxínu na funkčný vývin mozgu a správanie potomstva potkanov
Study of consequences of maternal depression and antidepressant venlafaxine treatment on functional development of the brain and behavior of rat offspring
Program: VEGA
Project leader: RNDr. Dubovický Michal CSc.
Duration: 1.1.2015 – 31.12.2018
Vplyv konštitučných faktorov redoxnej regulácie na endofenotypové znaky schizofrénie
The influence of constitutional factors of redox regulation on endophenotypic markers of schizophrenia
Program: VEGA
Project leader: MUDr. Riečanský Igor PhD.
Annotation:
Duration: 1.1.2016 – 31.12.2018
Vplyv veku na senzorickú reguláciu rovnováhy pri vstávaní zo sedu a chôdzi
Age-related changes in sensory control of balance during sit-to-stand and gait
Program: VEGA
Project leader: Ing. Hlavačka František CSc.
Annotation: The ability to reliably perform functional movements like sit-to-stand, gait or maintaining the erect posture is a basis for the independent living. Difficulties with conducting these motor activities increase with age. Early diagnostics and suitable therapeutic interventions can help to moderate these problems and prevent falls. The goal of our project is to obtain new knowledge about the physiological mechanisms of sit-to-stand movement and gait in young and elderly adults, and also to expand information about the sensory influence on these motor functions. We will focus on analysis of balance control in static and dynamic conditions during sit-to-stand, step initiation and gait using unique 6-camera motion capture system BTS SMART-DX. We assume that results of our project would be worthwhile in designing of perspective rehabilitative methods for elderly people with mobility deficit to improve their balance and functional mobility, and also to enhance diagnostics of early stages of neurological disorders.
Duration: 1.1.2016 – 31.12.2018
Vzťah medzi telesnou adipozitou a funkčnými vlastnosťami artérií u potkana
Relationship between body adiposity and functional properties of arteries in rat
Program: VEGA
Project leader: Mgr. Zemančíková Anna PhD.
Annotation: High levels of body mass index are considered as an important risk factor contributing to cardiovascular impairment. However, recent studies have brought some noteworthy findings that moderately increased amount of body fat is beneficial with respect to prognosis of patients with heart and vessel diseases. The aim of this project is to analyse the relationship between the level of body adiposity/weight and cardiovascular function in terms of blood pressure regulation and functional properties of selected arteries in rat. An effort will be made to reveal how the vessels are modulated by the adjacent perivascular adipose tissue (PVAT) in healthy normotensive and in hypertensive rats after moderate and considerable increase in body adiposity induced by dietetic interventions in different ontogenetic stages. Several biometric and hemodynamic parameters will be measured, as well as some biochemical parameters in blood and tissues and in vitro reactivity of arteries in the presence of PVAT and after its removal.
Duration: 1.1.2018 – 31.12.2018
KANASTA – Kardiovaskulárne účinky nanoenkapsulovaného simvastatínu a koenzýmu Q10 pri experimentálnej hyperlipidémii (KANASTA)
Cardiovascular Effects of Nanoencapsulated Simvastain and Coenzyme Q10 in Experimental Hyperlipidemia (KANASTA)
Program: Other projects
Project leader: RNDr. Reháková Radoslava
Annotation: The project is aimed to investigate cardiovascular effects of simvastatin together with CoQ10 in experimental hyperlipidemia and to increase bioavailability of simvastatin in the liver, thus reducing the daily dose and consequently to prevent the reduction of CoQ10 levels.
Duration: 27.11.2015 – 26.11.2018
Výskum inovatívnych liekových foriem a technologických postupov pri ekologickom spracovaní biologického odpadu vaječných škrupín
Program: Vedecko-technické projekty
Project leader: RNDr. Dubovický Michal CSc.
Duration: 1.9.2015 – 31.8.2018
Možná duálna funkcia P-glykoproteínu pri viacliekovej rezistencii leukemických buniek: efluxná pumpa a regulačný proteín.
Program: SRDA
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 1.7.2014 – 30.6.2018
Aldoketoreduktázy v chronických ochoreniach – in silico modelovanie významných enzýmov a ich komplexov s indolovými derivátmi
Aldo-keto reductases in chronic diseases – in silico modeling of significant enzymes and their complexes with indole derivatives
Program: VEGA
Project leader: RNDr. Májeková Magdaléna PhD.
Annotation: The aldo-keto reductases belong into a family of enzymes characterized as monomeric NADPH-dependentoxidoreductases. Till now the number of more than 100 enzymes from this family is known. They are divided according to their substrate specificity to individual subgroups. Apart from their beneficial physiological functions hold by biosynthesis, metabolism and detoxication, they can play also a negative role by various pathologic processes as chronic diabetic complications, inflammation, carcinogenesis, asthma etc. The aim of this work is toelaborate and test the specific models of significant subgroups of aldoketoreductases by means of in silico methods. The individual models are to be used for considering the inhibition properties of indole compounds with measured or perspective biological activity.
Duration: 1.1.2014 – 31.12.2017
Bioenergetické aspekty ochrany myokardu pomocou remote ischemického preconditioningu. Úloha srdcových mitochondrií
Bioenergetic aspects of myocardial protection by means of remote ischemic preconditioning. The role of cardiac mitochondria
Program: VEGA
Project leader: Ing. Ferko Miroslav PhD.
Annotation: Any sufficiently strong challenge induced by physiological or pathological stimuli leads to myocardial changes that can be considered as abnormal. These changes partially reflect endogenous protective processes. Pathological stimuli triggering the endogenous protection are hypoxia, ischemia, diabetes mellitus and hypertension. Cardioprotective approaches involve pharmacological and ischemic preconditioning (IP) as well as clinically applicable form of IP, so called remote ischemic preconditioning (RIP). Numerous mechanisms of RIP induction and effects have been investigated. Still, the character of the signals from the site of RIP leading to target structures in the heart is not elucidated. Little is known also with respect to bioenergetics aspects and role of cardiac mitochondria in RIP induced cardioprotection. The aim of this project is to contribute to elucidation of the molecular mechanisms of RIP and role of mitochondria in the signaling processes of RIP and heart adaptation to hypoxia and ischemia.
Duration: 1.1.2015 – 31.12.2017
Deriváty kyseliny 1-indoloctovej ako inhibítory aldózareduktázy: dizajn, syntéza a biologická aktivita
Indole-1-acetic acid derivatives as aldose reductase inhibitors: design, synthesis and biological activity
Program: VEGA
Project leader: Ing. Štefek Milan CSc.
Duration: 1.1.2015 – 31.12.2017
Matrix metaloproteinázy, microRNAs a deformabilita erytrocytov – nové diagnostické a prognostické biomarkery srdcového zlyhávania
Matrix-metalloproteinases, microRNAs and deformability of erythrocytes as a novel diagnostic and predictive biomarkers of heart failure
Program: VEGA
Project leader: doc. RNDr. Barteková Monika PhD.
Annotation:
Duration: 1.1.2014 – 31.12.2017
Molekulárne mechanizmy zahrnuté v účinkoch doxorubicínu u zvierat s rozvinutou hypertenziou a možnosti ovplyvnenia účinkov doxorubicínu pôsobením kvercetínu.
Molecular mechanisms involved in the effects of doxorubicin in rats with developed hypertension and ways of modulation of of these effects of doxorubicin by quercetin.
Program: VEGA
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 1.1.2015 – 31.12.2017
Nové molekulárne mechanizmy poškodenia kardiovaskulárneho systému ionizujúcim žiarením a možnosti jeho cielenej medikamentóznej prevencie.
Program: VEGA
Project leader: D.h.c., Prof., MUDr. Slezák Ján DrSc., FIACS
Duration: 1.1.2015 – 31.12.2017
Oxid dusnatý a redoxný stav mozgu v experimentálnom neurovývinovom modeli schizofrénie
Nitric oxide and brain redox status in an experimental neurodevelopmental model of schizophrenia
Program: VEGA
Project leader: RNDr. Vranková Stanislava PhD.
Annotation: Schizophrenia is a severe mental disorder. Research suggests that an important role its pathophysiology may play the oxidative stress, i.e., redox imbalance in neurons of the central nervous system, leading to an impairment of brain development. This redox imbalance might be caused by an overproduction of nitric oxide (NO). Therefore, this project is focused at the role of NO in brain development. We will adopt the postweaning social isolation rearing of rats, which is an established neurodevelopmental model of schizophrenia. We will explore whether social isolation affects NO synthesis in the brain and whether NO production is linked with redox status of the brain tissue and schizophrenia-like behavioral alterations. We will also explore whether the changes in neurobiology and behavior, induced by isolation rearing, can be positively influenced by an antioxidative treatment. Results of this project will contribute to elucidating the pathogenesis of schizophrenia and its treatment possibilities.
Duration: 1.1.2015 – 31.12.2017
Signálne dráhy NO a H2S a ich interakcia v regulácii cievneho tonusu počas skorej fázy vývoja experimentálnej hypertenzie
NO and H2S signal pathways and their interaction in the control of vascular tone during early developmental stage of experimental hypertension
Program: VEGA
Project leader: RNDr. Čačányiová Soňa PhD.
Annotation: Nitric oxide (NO) and hydrogen sulphide (H2S) belong to important gaseous molecules engaged to vascular smooth muscle regulation during normotensive as well as hypertensive conditions. In adult rats it is well documented the role of NO originated from endothelial isoform of NO-synthase, the specificity and originality of NO released from neuronal isoform of enzyme as well as the existence of specific NO-H2S interaction – not just yet identified nitroso-sulphide signalization. The aim of the project is to characterize and compare the role of NO and H2S, their signal pathways and interaction, in young normotensive rats and in rats with developing experimental hypertension(NO-deficient and essential hypertension. Simultaneous following of both signal pathways on the same vessels isolated from normotensive and hypertensive rats and humans represents the actual way how to associate the results of basic research with clinical praxis.
Duration: 1.1.2014 – 31.12.2017
Štúdium klinicky využiteľných foriem preconditioningu ako alternatívnej metódy ochrany myokardu pred akútnou ischémiou v organizme zaťaženom civilizačnými ochoreniami
Study of the clinically relevant forms of preconditioning as an alternative method of myocardial protection against acute ischemia in the organism challenged with civilization diseases
Program: VEGA
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Hypertension, metabolic disorders, sex and aging have a negative impact on the adaptive mechanisms of innate cardioprotection and alter heart survival upon acute ischemia/reperfusion (I/R). These comorbidities suppress the mechanisms of cell signaling involved in protective effects of ischemic preconditioning (PC) and accelerate necrotic and apoptotic processes during I/R, reduce energy production and contribute to enhanced arrhythmogenesis. The project is aimed to investigate the possiblities to restore adaptive potential in the pathologically altered myocardium including aged heart by means of modulation of the intensity and forms of adaptive stimuli. Effects of clinically relevant forms of PC: „remote“ PC of the heart induced by ischemia of the distant organ and its pharmacological simulations by drugs regulating energy metabolisms and involved in the mechanisms of PC, as well as their genomic and non-genomic effects including the impact on mitochondrial function and apoptotic cascade will be investigated
Duration: 1.1.2015 – 31.12.2017
Úloha perivaskulárneho tukového tkaniva v regulácii cievneho tonusu u potkanov s kardiovaskulárnou dysfunkciou
The role of perivascular adipose tissue in the regulation of vascular tone in rats with cardiovascular dysfunction
Program: VEGA
Project leader: Mgr. Zemančíková Anna PhD.
Annotation: Perivascular adipose tissue (PVAT) which surrounds most of the systemic arteries is considered to be an important modulator of their functions. Relaxant factors produced by this tissue inhibit vascular contractile responses to many vasoconstrictor substances. The aim of this project is to analyse the mechanisms which are responsible for this modulatory effect. In addition to the direct action of the PVAT-released substances on vascular smooth muscle we intend to study also the possible interaction of PVAT with vascular sympathetic nervous system. These interactions are important for maintaining vascular tone in physiological as well as in pathological conditions associated with vascular dysfunction, e.g. in hypertension. Our goal is to discover whether the changes in paracrine functions of PVAT may participate in the origin of vascular disorders and whether they precede the abnormalities of vascular reactivity at the level of smooth muscle.
Duration: 1.1.2015 – 31.12.2017
Vzťah kognitívnych schopností a funkčnej asymetrie mozgových heimsfér
Cognitive skills form the perspective of functional asymmetry of the brain
Program: VEGA
Project leader: RNDr. Cimrová Barbora PhD.
Annotation: Recent research using dichotic stimulation and a visual half-field technique indicate that sex differences in cognitive skills could be connected with lateralization of perceptual cognitive functions particularly because men and women differ not only in cognitive skills but also in degree of lateralization of cognitive functions. In general, perceptual asymmetries are greater in men. An assumtion is that a certain degree of the leftward lateralization (but not the maximal lateralization) is optimal for verbal functions, so that high left hemisphere lateralization is not associated with superior performance. Therefore, women outperform men in verbal tasks. In nonverbal task, a greater degree of lateralization towards the right hemisphere is associated with better performance. The proposed project will try to answer a question whether a degree of lateralization could explain the variation in performance within the groups of men and women. The sex differences in lateralization will be explored as well.
Duration: 1.1.2015 – 31.12.2017
Chemoenzymatická syntéza a hodnotenie biologických aktivít prírodných glykofenolík a ich analógov.
Chemoenzymatic synthesis and evaluation of biological activities of natural glycophenols and their analogues
Program: SRDA
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 1.10.2013 – 30.9.2017
Štúdium regulácie radikálovej a bunkovej signalizácie v hypertenzii a vplyv nových terapií na túto signalizáciu.
Study of regulation of radical and cellular signaling during hypertension and influence of novel therapies on this signaling
Program: SRDA
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 1.10.2013 – 30.9.2017
HYPERAD – Štúdium regulácie radikálovej a bunkovej signalizácie v hypertenzii a vplyv nových terapií na túto signalizáciu.
Study of regulation of radical and cellular signaling during hypertension and influence of novel therapies on this signaling.
Program: SRDA
Project leader: RNDr. Dovinová Ima PhD.
Annotation: New project was started in october 2013. This year we studied mechnisms involved in blood pressure regulation of young SHR after treatment of PPAR gamma agonist- pioglidazone (PIO). Application of PIO significantly influenced development of high blood pressure and improved lipid profile and vessels relaxation. PIO ifluenced also: i) regulation of SOD enzymes, ii) renin-angiotenzin system a iii) intracellular signaling pathways of Akt kinase and beta-catenin. Outputs: 1 publication CC.
Duration: 1.10.2013 – 30.9.2017
Ako synchronizácia formuje sociálne väzby: mechanizmy a neuronálne dráhy
How synchrony shapes human social bonding: mechanisms and neural pathways
Program: SASPRO
Project leader: MUDr. Riečanský Igor PhD.
Annotation: Human societies are characterized by flexibly changing social groups with remarkably strong bonds. A striking, universal characteristic of bonded groups is a widespread tendency their members to synchronize their movements. Such behavioral synchronization supports deindividuation, cooperation, and group solidarity. However, the psychological and neural mechanisms of the link between synchrony and bonding are poorly understood. This project aims to fill this gap by using a multi-level social neuroscience approach based on a series of behavioral, functional neuroimaging and psychopharmacological experiments. Insights from the project will advance our understanding of the complex processes of social behavior. The principal investigator of this project is Dr. Jasminka Majdandzic.
Duration: 1.5.2015 – 20.9.2017
Vekom podmienené zmeny vo funkcii endotelu v experimentálnej hypertenzii
Effect of aging on the endothelial function in experimental hypertension
Program: Other projects
Project leader: MUDr. RNDr. Púzserová Angelika PhD.
Annotation: The aim of this project is to investigate the impact of aging on the cardiovascular system, mainly the functional state of the endothelium in the large, medium-sized and resistance arteries during normotension and hypertension. The project studies the role of various endothelium-dependent relaxing and contracting factors in peri-pubertal, adult and senescent rats. In addition, we determine the effect of aging on deformability of red blood cells, nitric oxide bioavailability and reactive oxygen species in the heart and selected arteries.
Duration: 17.4.2014 – 17.4.2017
Skúmanie molekulárnych mechanizmov kardioprotektívnych účinkov melatonínu a omacoru pri vzniku metabolického syndrómu v experimentálnych podmienkach
Program: Other projects
Project leader: RNDr. Egan-Beňová Tamara PhD.
Duration: 2.2.2014 – 1.2.2017
Efekt oxidu dusnatého a sírovodíka na štruktúru a funkciu kardiovaskulárneho systému u normotenzných a hypertenzných potkanov
The efect of nitric oxide and hydrogen sulfide on structure and function of cardiovascular system in normotenzive and hypertenzive rats.
Program: VEGA
Project leader: RNDr. Kristek František DrSc.
Annotation: In blood pressure regulation are, besides clasical transmitters,engaged gaseous transmiters – mainly nitric oxide (NO) and new very perspective substance hydrogen sulfide (H2S). Completely new signal pathway seems to be neuronal NO synthase (nNOS) and its interactions with other regulatory systems including H2S. nNOS inhibits renin synthesis in macula densa cells and via it influences renin-angiotensin system, which is intimately related to sympathetic nervous system. Enzymes responsible for H2S production are also present in kidney and H2S is, beside others, engaged in renal blood flow and vessel wall tone regulation. The aim of the present project is to study the effect of nNOS and eNOS alone and/or in combination with H2S and other systems engaged in cardiovascular regulation during normotensive and hypertensive conditions. The availability of the results will be reached via complex functional (in vivo and in vitro), morphological, and biochemical approach.
Duration: 1.1.2013 – 31.12.2016
Epikatechín v prevencii včasného rozvoja primárnej hypertenzie: mechanizmy pôsobenia v kardiovaskulárnom a centrálnom nervovom systéme
Epicatechin in prevention of early development of primary hypertension: mechanisms of action in the cardiovascular and central nervous systems
Program: VEGA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation: This project investigates the effect of epicatechin (Epi) in the cardiovascular and central nervous systems of normotensive, borderline hypertensive (BHR) and spontaneously hypertensive rats as well as in the young humans. Epi has been given to rats in the age of 6-7 weeks which is a critical developmental window regarding development of blood pressure. The effect of Epi on behaviour, blood pressure and selected metabolic, functional and structural parameters of the brain, heart and arteries has been determined. The attention has been paid to the mechanisms of blood pressure regulation and vascular function mediated by nitric oxide, reactive oxygen species and endothelium-derived constricting factors. In young humans Epi has been administered in flavanol-rich chocolate and the reactivity of blood pressure during mental load was determined. The results allow us to evaluate the use of Epi in prevention of hypertension in rats with genetic predisposition to hypertension as well as in humans in conditions of mental load.
Duration: 1.1.2014 – 31.12.2016
Epikatechín v prevencii včasného rozvoja primárnej hypertenzie: mechanizmy pôsobenia v kardiovaskulárnom a centrálnom nervovom systéme
Program: VEGA
Project leader: RNDr. Sotníková Ružena CSc.
Duration: 1.1.2014 – 31.12.2016
Filtrovanie senzorických informácii u osôb s genetickým rizikom schizofrénie
Sensory information filteringin persons with genetic risk of schizophrenia
Program: VEGA
Project leader: MUDr. Jagla Fedor CSc.
Annotation: Schizophrenia is a devastating mental disorder, but its pathophysiology not clarified yet. An important role in the development of the disorder seems to play an insufficient filtration of irrelevant sensory stimuli. Genetic predisposition is the major risk factor for developing schizophrenia and research indicates involvement of common polymorphisms in multiple functionally coupled genes. There is increasing evidence that redox dysbalance importantly contributes to schizophrenia. In this project, we will use electrophysiological methods to assess how common variability in key enzymes regulating cellular redox status, neuronal nitric oxide synthase and glutamate cysteine ligase, is related to filtering auditory stimuli in humans. This project will provide important novel knowledge on the pathomechanism of schizophrenia.
Duration: 1.1.2014 – 31.12.2016
Funkčné testy v diagnostike posturálnej stability a sily svalov trupu
Functional tests in diagnostics of postural stability and strength of core muscles
Program: VEGA
Project leader: Ing. Hlavačka František CSc.
Annotation: The aim of the project is to design new methods for evaluation of postural stability and trunk muscles strength in static and dynamic conditions. The results will include testing protocols and recommendations for using the devices and medhots in praxis.
Duration: 1.1.2014 – 31.12.2016
Rozvoj SQUID gradiometrických a susceptometrických metód pre bioaplikácie spojené s homeostázou železa
Development of SQUID Gradiometric and Susceptometric Methods for Iron Homeostasis Related Bio-Applications
Program: VEGA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation: The aim of this project is to design and optimaze the SQUID gradiometric and susceptometric measuring methods and devices for detection and localization of the cancer tissue as well as for investigation of processes related to a disturbed iron metabolism in cardiovascular diseases. We investigate iron homeostasis and biomineralization in ferritin cores in above mentioned diseases. We also investigate the limit sensitivity of this measuring system. We identify the factors which affect the diagnostic interpretation of the results achieved.
Duration: 1.1.2013 – 31.12.2016
Účasť HMGB1 proteínu v experimentálnom infarkte myokardu: ochrana vs. poškodenie myokardu
Participation of HMGB1 in experimental myocardial infarction: cardioprotection vs. cardiac depression
Program: VEGA
Project leader: RNDr. Cebová Martina PhD.
Annotation: Since many of proteins expressed during myocardial infarction (MI) could have either protective or harmful effectson function and structure of myocardium, it is important to analyze their activity as well as extensity of expression.High-mobility group box 1 (HMGB1) has been recently found as a nuclear protein released during the cerebralischemic event and myocardial ischemia. The goal of this project is to determine the expression of HMGB1 afterexperimental MI and evaluate its effects. To analyze protective or by contrast damaging effects, the protein will beadded or blocked, respectively. In case of protection, the optimal dose of added protein will be analyzed. In caseof harmful effects, the protein will be blocked by both specific antibody or new Affibody molecules. The Affibodymolecules are smaller and penetrate more deeply into the tissue. Thus, better protective effects may beexpected. The results could be used to generate a drug characterized and optimized for subsequeuent clinicaldevelopment.
Duration: 1.1.2014 – 31.12.2016
Vlastnosti Na,K-ATPázy, jedného z kľúčových systémov pre udržiavanie koncentrácie sodíka v organizme, v podmienkach civilizačných ochorení, ako sú hypertenzia, diabetes mellitus a hypertriglyceridémia.
Response of the Na,K-ATPase, representing one of the crucial systems in maintaining the sodium homeostasis, to civilization diseases namely: hypertension, diabetes and hypertriglyceridemia.
Program: VEGA
Project leader: RNDr. Vrbjar Norbert CSc.
Annotation: The present project is oriented to obtain new data concerning the maintenance of intracellular homeostasis of sodium, representing one of the unavoidable factors for appropriate regulation of cellular viability. Various widely spread diseases, like hypertension, diabetes mellitus and hypertriglyceridemia are accompanied by disturbances in the maintenance of intracellular sodium homeostasis. Using in vivo models (rat) we will investigate the influence of above listed diseases on the adaptation of Na,K-ATPase which is one of the crucial systems in maintaining intracellular concentration of sodium ions. The data will contribute to elucidation of molecular background of processes involved in maintaining the cell’s viability in the heart, in the kidney and in the brain, from the aspect of the possible protection of the organism against hypertension, diabetes mellitus and hypertriglyceridemia.
Duration: 1.1.2013 – 31.12.2016
Vplyv agonistov PPAR gama na antioxidačnú odpoveď a na reguláciu radikálovej a bunkovej signalizácie v hypertenzii
Effect of PPAR gamma agonists on antioxidant response and on regulation of radical and cell signaling in hypertension
Program: VEGA
Project leader: RNDr. Dovinová Ima PhD.
Annotation: An important factor in human and experimental hypertension is the excesive oxidative load. The oxidative stress up-regulates antioxidant response (phase II response) by activation of transcription factor Nrf2. Application of substances modulating the expression of such antioxidant response may be beneficial in reduction of blood pressure and in regression againts blood vessels and other tissues damage. An important role in processes asociated with free radicals and development of hypertension is activation of intracellular signaling, which includes kinase pathways. The PPAR gamma agonists, using dominantly in diabetes melittus therapy, seem to be helpfull also in regulation of hypertension and are important in studying„cross-talk“ among Nrf2 a PPAR gamma. The aim of the project is to search effect of short and long therm therapy of PPAR gamma agonists on antioxidant response, blood vessels and heart damage and on find out the molecular mechanisms.
Duration: 1.1.2014 – 31.12.2016
NODAGATE – Vplyv variability génov NOS1 a DAT1 na senzomotorický gating u človeka: implikácie pre etiopatogenézu schizofrénie
The influence of variability of NOS1 and DAT1 genes on sensorimotor gating in humans: the implications for the pathophysiology of schizophrenia
Program: Vedecko-technické projekty
Project leader: MUDr. Riečanský Igor PhD.
Annotation: Research shows that interactions of functionally related genes play major role in the pathogenesis of schizophrenia. In this project, we investigate the association between combined polymorphisms of NOS1 a DAT1 genes and sensorimotor gating, a endophenotype of schizophrenia. The project will yield novel knowledge on the regulatory mechanisms of dopaminergic signaling, importantly involved in schizophrenia.
Duration: 1.1.2013 – 31.12.2016
MVTS Integrovaná európska platforma pre výskum rakoviny pankreasu: od základného výskumu ku opatreniam v klinickej medicíne a verejnom zdravotníctve v oblasti zriedkavých chorôb
MVTS An integrated European platform for pancreas cancer research: from basic science to clinical and public health interventions for a rare disease
Program: Other projects
Project leader: RNDr. Májeková Magdaléna PhD.
Annotation: To present the method based on molecular modeling, which is connected with potential molecular target for pancreas cancer detection, treatment and prevention. The aldo-keto reductases (AKRs) are enzymes with beneficial physiological functions, but they can play also a negative role by various pathologic processes. Our aim is to elaborate models of AKR subtypes specific for pancreatic cancer, drug design and characterization of novel aldo-keto reductase inhibitors of indole type with potential therapeutic effect in relation to pancreatic cancer and to evaluate their use together with biochemical experiments.
Project web page: http://www.cost.eu/domains_actions/bmbs/Actions/BM1204
Duration: 1.6.2013 – 13.12.2016
NPPOSC – Národný program prevencie ochorení srdca a ciev – Zdravé srdce pre Slovensko časť pre deti a dorast
National Programme for Prevention of Cardiovascular Disease
Program: Other projects
Project leader: RNDr. Regecová Valéria
Annotation: Cardiovascular program for children and adolescents is a part of the National Programme for prevention of cardiovascular disease (NPPCVD) in SR and is also included in the contract on cooperation between the Ministry of Health and the WHO Regional Office for Europe.Basic objectives and means NPPCVD for children and adolescents: Healthy Heart for Slovakia:• Determine the real incidence of the early stages of cardiovascular disease,in the Slovak Republic in children• Determine the prevalence of risk factors (RF) in children and adolescent of Slovakia • Put in place uniform diagnostic and therapeutic procedures for CVD• Find the control mechanisms for the implementation of preventive and therapeutic procedures• Start with preventive action from early childhood.Final goals NPPCVD:• Reduce the incidence of cardiovascular disease,• Reduce the number of complications from cardiovascular disease,• Reduce mortality and morbidity from cardiovascular disease.
Duration: 1.12.2011 – 1.12.2016
Dobudovanie infraštruktúry pre moderný výskum civilizačných ochorení
Program: EU Structural Funds Research & Development
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 27.10.2015 – 31.12.2015
Farmakologická regulácia aktivity a apoptózy fagocytov: štúdium na celulárnej a molekulárnej úrovni
Pharmacological regulation of phagocyte activity and apoptosis: studies on cellular and molecular levels
Program: VEGA
Project leader: RNDr. Drábiková Katarína PhD.
Duration: 1.1.2013 – 31.12.2015
Kinematická analýza postoja a chôdze u zdravých ľudí a pacientov s poruchou rovnováhy.
Kinematic analysis of posture and gait in healthy subjects and patients with balance impairment.
Program: VEGA
Project leader: Ing. Hlavačka František CSc.
Annotation: Kinematics of posture and gait including physiological motion analysis plays an important role in relation toneurological and motor disorders.The goal of our project is to bring new knowledge about mechanisms of humanbalance control, gait dynamics and step initiation, and also to expand information about sensory influence onthese functions.The objective of proposal is to analyse mechanisms of sensorimotor control during step initiationand gait using unique 6-cameras motion capture system BTS SMART-DX. Postural sway of body segments willbe measured also by 3D accelerometers placed on the body. Data will be used for description of initial phase ofgait and subsequent gait cycle in healthy subjects.We will focus on age-related differences in motion kinematics.The aim of our project is to compare posture and gait kinematic parameters in healthy individuals and patientsand to find methods for detection of early stages of neurological disorders with sensory impaired balance,sensory deficits and unsafe gait.
Duration: 1.1.2013 – 31.12.2015
Kvasinky a ich možnosti pri ochrane integrity medzibunkových spojení vaskulárneho endotelu pred poškodením vyvolaným zápalom
Yeasts in protection of endothelial intercellular connections against inflammation-induced injury
Program: VEGA
Project leader: RNDr. Sotníková Ružena CSc.
Duration: 1.1.2013 – 31.12.2015
Kvasinky a ich možnosti pri ochrane integrity medzibunkových spojení vaskulárneho endotelu pred poškodením vyvolaným zápalom.
Yeasts in protection of endothelial intercellular connections against inflammation-induced injury.
Program: VEGA
Project leader: RNDr. Okruhlicová Ľudmila CSc.
Annotation:
Duration: 1.1.2013 – 31.12.2015
Multifunkčné pôsobenie kalcium/kalmodulín dependentnej proteínkinázy II (CaMKII) v srdci: vzťah k poruchám rytmu, kontraktility a bunkovej smrti.
Multifunctional activity of calcium/calmodulin dependent protein kinase II (CaMKII) in the heart: a relevance to disturbances in heart rhythm, contractility and cellular death.
Program: VEGA
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Role of Ca2+/calmodulin dependent protein kinase II (CaMKII) is controversial in excitacion-contraction coupling. It is not clear if activation or inhibition of CaMKII improves the heart function. Duration, type of injury and selective influence on the cytoplasmic/nuclear isoform dC,B determine the final action of CaMKII. The effects of CaMKII inhibition on arrhythmias induced by catecholamines, ischemia, ischemia/reperfusion and on cardiac performance will be studied. With respect to ECC in these pathological conditions, the protein content of CaMKIIdB,C, LTCC, NCX, PLB will be investigated. As CaMKII affects Ca2+ levels, which in turn influence apoptosis, we assume that the modulation of CaMKII may also determine the extent of cell death. It will be studied imunohistochemically and by measuring CaMKII activity, protein expression of caspases, Apaf-1, calpaine, Cyt C oxidase. The effects of the concomitant inhibition of CaMKII and beta-receptors, or RAAS, the main activators of CaMII, will be also investigated.
Duration: 1.1.2012 – 31.12.2015
Poškodenie zdravého tkaniva srdca a ciev pri ožiarení protónmi – patofyziológia a prevencia.
Proton radiation-induced cardiovascular toxicity – pathophysiology and prevention.
Program: SRDA
Project leader: D.h.c., Prof., MUDr. Slezák Ján DrSc., FIACS
Duration: 1.7.2012 – 31.12.2015
Prenatálne programovanie psychiatrických porúch: experimentálne možnosti hodnotenia mechanizmov vzniku psychiatrických porúch na animálnych modeloch
Prenatal programming of psychiatric diseases: experimental approaches for evaluation of causes and mechanisms of their origin
Program: VEGA
Project leader: RNDr. Mach Mojmír PhD.
Annotation: Incidence of mental diseases in the developed countries has an increasing trend. At least one mental disease occurred per year approximately in 27% of EU inhabitants (more than 82 mil. people). It is estimated that till 2020, depression will be the main cause of morbidity in the developed countries. There are many evidences on neurodevelopmental origin of mental diseases. Various environmental and maternal factors acting during prenatal period and early childhood can increase sensitivity of the individual to anxiety, depression or other mental disorders in later postnatal life. Insufficient oxygen and nutrition supply of tissues, excessive stress or chemical substances and drugs can adversely affect the development of the brain. Objective of the project proposal will be the evaluation of key epigenetic factors which may play an important role in development of mental diseases.Up-to-date molecular biology as well as non-invasive methods of ethological analyses of appropriate animal models will be utilized.
Duration: 1.1.2012 – 31.12.2015
Signálna dráha oxidu dusnatého a sírovodíka, jej poruchy a podiel na vzniku hypertenzie a aterosklerózy
Program: Other projects
Project leader: RNDr. Kristek František DrSc.
Annotation:
Duration: 19.7.2013 – 31.12.2015
Srdcové konexiny ako cieľové štruktúry pre prevenciu malígnych arytmií.
Program: VEGA
Project leader: RNDr. Tribulová Narcisa DrSc.
Duration: 1.1.2012 – 31.12.2015
Starnutie mozgu a neuroprotektívne antioxidanty: Ovplyvnenie glií ako terapeutická stratégia?
Program: VEGA
Project leader: Ing. Račková Lucia PhD.
Duration: 1.1.2012 – 31.12.2015
Ochrana srdca pr – Śtúdium ochrany srdcového svalu proti poškodeniu a malígnym poruchám rytmu vyvolaným zmeneným tyroidným stavom
Investigation of the cardioprotection using n-3 PUFA against injury and malignant arrhythmias induced by altered thyroid status.
Program: SRDA
Project leader: RNDr. Tribulová Narcisa DrSc.
Annotation: The purpose of the proposed project is the bilateral collaboration enabling effective use ofmethodological and instrumental facilities of each laboratory with the following scientific aims.The main goal is to evaluate whether supplementation of anti-arrhythmogenic n-3polyunsaturated fatty acids (n-3 PUFA) with known protective effects in patients will havebeneficial effect in our experimental model of rats with altered thyroid hormone levels, and tocontribute to our knowledge of factors helping in protection against heart damage and malignantarrhythmias with the following detailed aims:i) to follow frequency of arrhythmias after supplementation of protective fatty acids by the methodof perfused heart under control and modified conditions;ii) to analyze changes at the protein and mRNA levels of connexin channels and PKC-εsignalization in connection with heart sensitivity to malignant arrhythmias, and to verify ourhypothesis suggesting that modulation of PKC-ε signalization caused by thyroid hormones in theheart plays an important role in occurrence of malignant arrhythmias;iii) to analyze changes in MyHC composition and to verify our hypothesis suggesting thatdifferences in MyHC isoform expression can influence occurrence and frequency of malignant arrhythmias.
Duration: 1.1.2014 – 31.12.2015
H2S – Štúdium molekulárnych mechanizmov biologických účinkov H2S.
Study of molecular mechanisms of H2S biological effects.
Program: SRDA
Project leader: RNDr. Čačányiová Soňa PhD.
Annotation: Recently, endogenously produced hydrogen sulphide (H2S) is recognized as the third signál molecule-gasotransmitter. Thus H2S influences numerous biological processes, e.g. neuromodulation, proliferation, apoptosis, regulation of heart, cardioprotection, ischemia-reperfusion, hypertension, vasorelaxation, septic and hemorrhagic shock, inflammation processes, penile erection, hibernation or atherosclerosis, yet its molecular mechanism is not fully understood. The aim of the project is to understand the numerous biological effects of H2S. We will study how H2S releases NO from endogenous NO-donors and how cysteine, glutathione and other biologically active drugs influence the NO release. WhetherH2S canbind to proteins and release NO from endogenous NO-donors. H2S together with NO-donors will be studied on activities of K+, Cl- and Ca2+ membrane channels and on the contraction/relaxation of aorta in vitro, rat heart beat and blood pressure. Because H2S acts as a neuromodulátor, it is involved in neuroprotection but also in neurodegenerative processes. On neuronal cells, we will study three H2S-producing enzymes and their changes at the level of mRNA géne expression and at the level of proteins under normál and pathophysiological conditions. The results of these studies may explain the H2S numerous biological effects and extend the knowledge on mechanism of the H2S actions to šuch level that the results could be used as support for application studies of H2S in medicíne.
Duration: 1.7.2012 – 31.12.2015
Vplyv genetickej kontroly tvorby oxidu dusnatého a spätného vychytávania dopamínu na senzoricko-motorické vrátkovanie u človeka
The influence of genetic control of nitric oxide production and dopamine re-uptake on sensorimotor gating in humans
Program: VEGA
Project leader: MUDr. Riečanský Igor PhD.
Annotation: Schizophrenia is a severe mental disorder, but its pathophysiology is poorly understood. A major role in its etiopathogenesis seem to play interactions of functionally coupled genes. One of core neurobiological abnormalities is increased synaptic dopamine in the striatum. This is also linked with disrupted filtering of behaviorally irrelevant stimuli, so called sensorimotor gating, which is an important schizophrenia intermediate phenotype. Nitric oxide (NO), a gaseous neurotransmitter, inhibits re-uptake of dopamine by blocking dopamine transporter (DAT1). Variability of both DAT1 and neuronal nitric oxide synthase (NOS1), the enzyme mediating NO production, is associated with schizophrenia risk. In this project we will explore how combination of common functional polymorphisms of NOS1 and DAT1 determines gating in healthy human subjects. This project will yield novel knowledge on the mechanisms regulating dopaminergic signaling, which may be of great importance in the pathophysiology of schizophrenia.
Duration: 1.1.2013 – 31.12.2015
Vplyv chronického stresu na proliferáciu srdcových buniek.
The effect of chronic stress on cell proliferation in the heart
Program: VEGA
Project leader: doc. RNDr. Barteková Monika PhD.
Annotation:
Duration: 1.1.2012 – 31.12.2015
RIFADMY – Vplyv rizikových faktorov súvisiacich so životným štýlom na adaptačné procesy v ischemickom myokarde
The effect of the lifestyle-related risk factors on the adaptive processes in the ischemic myocardium
Program: SRDA
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Lifestyle-related risk factors (RF) including, besides elevated blood pressure, hyperglycemia and dyslipidemia have a negative impact on the heart exposed to ischemia: they promote its lethal injury (infarction) and the occurrence of sudden death due to malignant ventricular arrhythmias. On the other hand, some stressful factors including free radicals and increased glucose levels may play a dual role in the mechanisms of ischemia-reperfusion injury (IRI) and induce, except deleterious effects, adaptive processes stimulating cardiac endogenous mechanisms of resistance against IRI. Increased ischemic tolerance that can be evoked by the pleiotropic actions of some hypolipidemics and antidiabetic drugs is also characteristic for female myocardium, however, it declines with age in both genders. The efficiency of adaptation may be reduced by comorbidities related to lifestyle, although the effect of RF has not been definitively proven. It has been shown that even pathologically altered myocardium need not completely loose its ability to be adapted. We aim to verify the hypothesis that lifestyle RF alter myocardial response to acute ischemia not only via interference with the pathophysiological mechanisms of IRI but also via suppression of the intrinsic adaptive mechanisms in the myocardium and its ability to tolerate ischemic challenge. The project is focused on the study of the mentioned RF on the cellular protective mechanisms in relationship with gender differences and age, as well as on the exploration of the possibilities to inhibit unwanted effects of RF and to restore the lost adaptive potential of the myocardium.
Duration: 1.7.2012 – 31.12.2015
RIFADMY – Vplyv rizikových faktorov súvisiacich so životným štýlom na adaptačné procesy v ischemickom myokarde
The effect of lifestyle-related factors on the adaptive processes in ischemic myokardium
Program: SRDA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.7.2012 – 31.12.2015
Zlyhanie mozgového energetického metabolizmu v patobiochemickom mechanizme hypoxicko-ischemického poškodenia mozgu novorodencov
Cerebral energy metabolism failure as one of patho-biochemical mechanisms involved in hypoxic-ischemic insult of the neonatal brain
Program: VEGA
Project leader: RNDr. Juránek Ivo PhD., DrSc.
Annotation: The main purpose of the project is to contribute for elucidation of mechanisms of hypoxic-ischemic damage (HID) of the neonatal brain, which is cause of ~50% of newborn deaths. Survived infants often suffer from chronic neuro-psychiatric disorders (e.g. cerebral palsy, epilepsy, cognitive and behavioral disorders). Secondary energy failure (SEF) is likely to play a key role in the evolving HID. In our previous studies, we found that primary energy failure (PEF) in the brain of newborn rats is proportional to the maturity of brain energy metabolism. Since SEF determines the severity of brain HID, our goals for the present project are: 1) to monitor HID of the neonatal rat brain; 2) to determine the time interval between PEF and SEF, as we propose its usage for an effective therapy; 3) to affect pharmacologically SEF. We expect that the inhibition of SEF should alleviate the process of brain HIP. We shall utilize biochemical, molecular-biological and, in particular, non-invasive MR approaches.
Duration: 1.1.2012 – 31.12.2015
CM1103 Štrukturálne podmienené navrhovanie liečiv na diagnózu a liečenie neurologických ochorení
CM1103 Structure-based drug design for diagnosis and treatment of neurological diseases
Program: Other projects
Project leader: RNDr. Májeková Magdaléna PhD.
Annotation: Based on our knowledge in design and modeling of indole compounds with neuroprotective effect and inhibition effect towards aldo-keto reductases we plan to use a multi-target potential of these compounds for the problems studied in the project COST CM1103. Our aims are:1) To elaborate molecule models for individual types of aldo-keto reductases (AKRs) important for neurological diseases.2) To suggest AKR inhibitors with indolic structure with optimal efficiency and bioavailability.3) To test new compounds as the inhibitors of enzymes important for monoamines metabolism.4) Structure-activity study.5) To test other effects of our compounds on an in vitro level in the framework of COST CM1103 collaboration.
Duration: 1.1.2014 – 27.11.2015
NOREG – Centrum excelentnosti pre výskum regulačnej úlohy oxidu dusnatého v chorobách z civilizácie
Centre of excellence for examination of regulatory role of nitric oxide in civilization diseases
Program: Centrá excelentnosti SAV
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.8.2011 – 30.6.2015
NOREG – Centrum excelentnosti SAV pre výskum regulačnej úlohy oxidu dusnatého v chorobách z civilizácie
Centre of excellence of SAS for research of regulatory role of nitric oxide in civilization diseases
Program: Centrá excelentnosti SAV
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation:
Duration: 1.8.2011 – 30.6.2015
Charakteristika kardiovaskulárnych a metabolických zmien v podmienkach fruktózou vyvolaného metabolického syndrómu u potkana
Characterization of cardiovascular and metabolic changes in fructose-induced metabolic syndrome in the rat
Program: VEGA
Project leader: doc. MUDr. Török Jozef CSc.
Annotation: The aim of this project is to evaluate the extent of cardiovascular impairments in metabolic syndrome induced by excessive intake of fructose which is considered to be an important obesitogenic factor in industrially developed countries. In fructose-induced rat model of metabolic syndrome, our effort will be oriented to reveal negative effects of long-term excessive intake of fructose on cardiovascular system with emphasis to participation of increased activity of peripheral sympathetic system, lipotoxicity and oxidative stress. We will analyse the intensity of fructose-induced changes in genetically related rat strains with different predisposition to individual components of metabolic syndrome. At the same time, we will examine the effect of long-term administration of antihypertensive and hypolipidemic drugs on hemodynamic and metabolic effects in rats fed with fructose. The results obtained in rats with long-term excessive intake of fructose could be used in evaluation of cardiovascular risk in obese individuals.
Duration: 1.1.2012 – 31.12.2014
Mechanizmy zahrnuté v účinkoch doxorubicínu na živočíšne bunky a hľadanie možností ovplyvňovania účinkov doxorubicínu.
Mechanisms involved in the effects of doxorubicin on animal cells and searching for possibilities of modulation of doxorubicin-induced effects.
Program: VEGA
Project leader: RNDr. Barančík Miroslav DrSc.
Annotation: Doxorubicin (DOX) is one of the most frequently drugs used in chemotherapy. However, systems influenced by DOX account for realization of both anticancer and toxic effects. The latter effects of DOX ivolve injury of several organs and are complications in its use. Project will be focused on the study of changes connected with action of DOX in different kinds of animal cells (normal tissue, leukemic cells). The differences and common characteristics in molecular mechanisms involved in DOX effects in these cells will be clarified. The changes in mitochondrial function, regulation of apoptosis and role of intra- and inter-cellular signaling (Akt kinase, JAK/STAT, MMP, connexins) will be determined. The tolerance of hearts against ischemia/reperfusion injury after DOX application will be determined. By the study of possibilities to modulate DOX effects, omega-3-fatty acids and flavonoids will be used. Studies will be performed at both in vivo and in vitro models, at the organ, cellular and subcellular levels.
Duration: 1.1.2012 – 31.12.2014
Modulácia kalciových púmp na úrovni sarkoplazmatického retikula (SR). Erytrocytov (RBCs) a pankreatických beta-buniek vo vzťahu k diabetu
Program: VEGA
Project leader: RNDr. Horáková Ľubica PhD.
Duration: 1.1.2011 – 31.12.2014
Molekulové modelovaníe, syntéza a biologická aktivita substituovaných pyridoindolov ako bifunkčných agens v prevencii diabetických komplikácií
Molecular modeling, synthesis and biological activity of substituted pyridoindoles as bifunctional agents in prevention of diabetic complications
Program: VEGA
Project leader: Ing. Štefek Milan CSc.
Duration: 1.1.2011 – 31.12.2014
Nový model experimentálnej hypertenzie, remodelácie ľavej komory a srdcového zlyhania indukovaný inhibíciou transkripčného nukleárneho faktora kappa B (NF-kB): protekcia melatonínom a kaptoprilom.
New model of experimental hypertension, left ventricular remodeling and heart failure induced by nuclear factor kappaB inhibition: pprotection by melatonin and captopril
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Annotation: Inhibition of nuclear factor kappaB induced left ventricular remodeling. The aim of the project is to study mechanisms leading to the remodeling and high blood pressure. Possible protective role of melatonin and ACE inhibitor – captopril will be studied as well.
Duration: 1.1.2012 – 31.12.2014
Skúmanie vplyvu melatonínu, omega-3 mastných kyselín a aliskirenu na myokardiálny konexin-43 a funkciu srdca u experimentálneho potkana s KVO.
Effects of melatonin, omega-3 fatty acids and aliskiren on myocardial connexin-43 and heart function in rats with CVD.
Program: Other projects
Project leader: RNDr. Tribulová Narcisa DrSc.
Annotation:
Duration: 12.10.2012 – 31.12.2014
Štúdium kombinácie imunosupresívnej liečby a ovplyvnenia redoxnej rovnováhy organizmu na zvieracích modeloch reumatoidnej artritídy
Study of combination of immunosuppressive treatment and substances affecting redox balance of organism on animal models of rheumatoid arthritis
Program: VEGA
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Annotation: The focus of the project will be the study of the therapeutic potential of the substances with anti-oxidative properties within RA therapy, and in particular of MTX combined therapy. The adjuvant arthritis (AA) model will be used in Lewis rats. AA progress and its pharmacological influencing will be characterized by parameters expressing immunological, oxidative, and inflammatory processes. The chronic AA model will be supplemented by the model of inflammation induced by carrageenan, as well as by the study of substances evaluated in macrophage cell culture.For potential utilization in combined RA therapy, the following substances will be evaluated: carnosine and its acetyled derivative, natural polysaccharides, low-molecular antioxidants with thiol groups, herbal antioxidants, and anti-inflammatory antihistaminics. Evaluation in vivo of the aforementioned substances will take place after the in vitro analysis of the anti-oxidative efficiency in two independent models
Duration: 1.1.2011 – 31.12.2014
Štúdium pôsobenia reaktívnych foriem kyslíka a dusíka na vysokomolekulový hyalurónan, synoviocyty a chondrocyty
Study of Actions of the Reactive Oxygen/Nitrogen Species on High-Molar-Mass Hyaluronan, Synoviocytes, and Chondrocytes
Program: VEGA
Project leader: Ing. Šoltés Ladislav DrSc.
Annotation: Hyaluronan (HA) is a polysaccharidic constituent of numerous tissues in the vertebrate organisms. One ml of synovial fluid (SF) – an essential joint component – contains 2-3 mg HA, which molar mass reaches in healthy adults the magnitude of about several megaDaltons. However, at the inflammatory joint disorders, the mean molar mass of HA is significantly decreased. This decrease is accompanied by a pronounced decline of the HA solution viscoelasticity and loss of the lubricating properties of SF.Low resistance of HA against the action of oxidative species – free radicals, anions – stimulated our efforts to use this biopolymer as a relevant (endogenic) substance for in vitro investigations of the “damaging” action of oxidants and/or for evaluation of the efficacy of various compounds/drugs to act as scavenging antioxidants.The substances, which will have significant antioxidant effect against HA degradation will be tested in relation to their protective effect against damage of joint elements.
Duration: 1.1.2011 – 31.12.2014
REINOC – Účinok antagonistu renínového receptora (RER-24) viazaného na nanonosiče pri experimentálnej hypertenzii
Effect of (pro)renin antagonist (RER-24) loaded naoparticles in experimental hypertension
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Annotation: Binding of prorenin to (pro)renin receptor leads to its activation via a non-proteolytic mechanism and to local generation of angiotensin I. RER-24 is a highly specific (pro)renin receptor antagonist with good affinity to(pro)renin receptors of all tissues. The simultaneous blockade of regulative as well as pathologic effects of(pro)renin receptors may however represent a limitation of RER-24 use. The aim of this project is to target inhibition of angiotensin I production directly to the organs mostly suffered from increased blood pressure – to the heart and kidney which could protect also the structure of tissues. To achieve this aim nanoencapsulation of RER-24 and magnetic nanoencapsulation will be performed followed by application and biological analysis of encapsulated RER-24 forms. While encapsulation assures increase of bioavailability, magnetization amplifies direct delivery of RER-24 to the target tissues. By this way cardio- and renoprotective effect beside blood pressure reduction will be reached.
Duration: 1.1.2012 – 31.12.2014
Účinok pyridoindolových derivátov v podmienkach experimentálneho modelu neurodegenerácie
Effect of pyridoindole derivatives under conditions of the experimental model of neurodegeneration
Program: VEGA
Project leader: RNDr. Gáspárová Zdenka PhD.
Annotation: Stroke and brain ischemia occur more frequently in the aging population. In our last project we studied consequences of acute ischemic brain injury and the effect of pyridoindoles related to age. The aim of this project is to study the effect of pyridoindoles in the model of neurodegeneration induced by trimethyltin. Neurodegeneration is associated with oxidative injury and inflammation of CNS. Functional and morphologicalchanges, loss of neurons, activated microglia, increased level of pro-inflammatory compounds and markers of oxidative stress are associated with many diseases, as Alzheimer´s and Parkinson´s disease, multiple sclerosis, etc. Neurodegeneration is connected with memory trace injury and it aggravates the action to other stressors, e.g.ischemic brain injury. The aim of the new project is to study the effect of pyridoindole antioxidants with the prospect of using them in slowing down the progress of pathological events accompanied with neurodegenerative changes in CNS.
Duration: 1.1.2011 – 31.12.2014
Úloha mitochondrií v adaptácii energetiky srdca na rôzne patologické podnety a noxy: ischémia, diabetes, hypertenzia
The role of mitochondria in adaptation of cardiac energetics to various pathological stimuli and noxae: ischemia, diabetes, hypertension
Program: VEGA
Project leader: Ing. Ferko Miroslav PhD.
Annotation: It is accepted that both, physiologically tolerable challenge and numerous pathological signals trigger endogenous defensive response in the heart leading to increased resistance or adaptation to a respective impuls. However, molecular mechanisms of adaptive changes are not yet sufficiently clarified. Basically they may appear as abnormalities difficult to distinquish from pathological alterations and may be sometimes improperly considered as therapeutic targets. With this respect, major attention deserve stimuli such as ischemia, diabetes, hypertension and delayed response to „remote preconditioning“ that has some clinical applications. In these conditions, adaptive response to altered tissue energy demand is less studied. Thus, our goal is to elucidate adaptive regulation of oxidative phosphorylation in relationship to changes in the properties of cardiac mitochondrial membrane, fluidity, oxidation of lipid bilayer, activity of mitochondrial ATP-ase and cytochromoxidase, under aforementioned states.
Duration: 1.1.2012 – 31.12.2014