Previous Projects

Completed projects over the past five years.

International

EU-CARDIOPROTECT – Realizácia terapeutického potenciálu nových kardioprotektívnych terapií
Realising the therapeutic potential of novel cardioprotective therapies
Program: COST
Project leader: MUDr. Ravingerová Táňa, DrSc., FIACS
Annotation: The proposed COST Action will set up a pan-European Research Network of leading experts in cardioprotection, to jointly develop new initiatives and new strategies for finding innovative and more effective approaches to cardioprotection and for optimizing the pre-clinical and clinical evaluation of new cardioprotective therapies, so as to improve their translation into the clinical setting for patient benefit. The COST Action will co-ordinate and strengthen European research in the field of cardioprotection and accelerate scientific progress through the dissemination and sharing of new therapeutic targets, among network members and industrial partners, thereby facilitating the discovery of new cardioprotective therapies. By utilizing the joint expertise of different European network members we will investigate factors which confound the efficacy of new cardioprotection therapies including comorbidities (such as age, diabetes, and hypertension) and co-medications (such as anti-platelet therapies, statins and beta-blockers). Finally, we will set up a European network of research centers for multi-center laboratory testing of new cardioprotective therapies using small and large animal models of acute IRI in order to select those therapies most likely to succeed in the clinical setting. All aspects of this COST Action proposal require a critical mass of partners across a wide geographic distribution across Europe in order to deliver the objectives outlined in this proposal. The discovery of novel signaling pathways and targets underlying cardioprotection both within and outside the cardiomyocyte (WG1 NEW TARGETS), and the testing of different combinations of cardioprotective therapy (WG2 COMBINATION THERAPY) requires investigators with different experience and expertise across Europe. The ability to test the effect of confounders of cardioprotection (WG3 CONFOUNDERS) requires the expertise of different partners in the different co-morbidities and testing of co-medications. Finally, the most important objective of this COST Action proposal, requires the setting up of a Europe-wide research network for (a) multicenter testing of novel cardioprotective therapies using small and large animal models (WG4 CONSORTIUM) and (b) testing of novel cardioprotective therapies in proof-of-concept clinical studies and optimization of multi-center clinical outcome cardioprotection studies. By definition this requires a critical mass of research partners distributed across Europe.
Duration: 19.10.2017 – 18.4.2022
Anti-inflammatory effect of astaxanthin, sulforaphane and Crocus sativus extract evaluated in two rodent models of age related diseases.
Program: Inter-academic agreement
Project leader: PharmDr. Bauerová Katarína, PhD., DrSc.
Duration: 1.1.2018 – 30.6.2021
Skúmanie mechanizmov antiarytmických účinkov melatonínu.
Investigation of the mechanims involved in antiarrhythmic effects of melatonin
Program: Bilateral – other
Project leader: RNDr. Tribulová Narcisa, DrSc.
Duration: 1.5.2014 – 31.12.2020
Śtúdium mechanizmov spúšťania a prenosu kardioprotektívných signálov indukovaných neinvazívnymi adaptačnými stimulmi
Study of the triggering mechanisms and transmission of cardioprotective signals induced by noninvasive adaptive stimuli
Program: Inter-academic agreement
Project leader: MUDr. Ravingerová Táňa, DrSc., FIACS
Annotation: Cardiovascular diseases are one of the leading causes of mortality in modern society and predicted to rise over the coming decades, due to aging population, longer survival after myocardial infarction, and incidence of civilization diseases. Research pointed out to the protective effects of phenomenon termed ischemic preconditioning (IPC), especially its novel clinically acceptable and safer forms. Currently, cellular mechanisms activated by stimuli like exercise, hypoxia and PC of the remote organ are not yet completely clear as compared with the classiccal IPC. For that reason, pathological animal models (myocardial ischemia, hypertension, d. mellitus, dyslipidemia) and aged animals will be used. Acute PC-like settings of above mentioned interventions, and their longer lasting adaptation modalities will be tested in the in vivo and the ex vivo rats using relevant methodology (combination of physiological, pharmacological and biochemical techniques). The results obtained in this project may lead to development of novel or modified therapeutic strategies to manage myocardial ischemia in patients.
Duration: 1.1.2018 – 31.12.2020
Study the role of iron oxide nanoparticles in a model of hypertension and comorbid Alzheimer\’s disease
Study the role of iron oxide nanoparticles in a model of hypertension and comorbid Alzheimer\’s disease
Program: Inter-academic agreement
Project leader: RNDr. Bernátová Iveta, DrSc.
Duration: 1.1.2018 – 31.12.2020
MuTaLig – Viac-cieľový model pre inovatívnu identifikáciu látok v procese objavovania liečiv
Multi-target paradigm for innovative ligand identification in the drug discovery process
Program: COST
Project leader: RNDr. Májeková Magdaléna, PhD.
Annotation: The aim of this COST Action is to join highly-qualified research teams working in disciplines around the field of medicinal chemistry, into a novel network devoted to the multi-target issue in drug discovery. The choice of this theme is related to its marked multidisciplinary character, which can ensure a strong interaction among all COST Action participants. Currently, an important and emerging issue in modern drug discovery is to design novel or identify existing bioactive compounds, endowed with the capability to interact selectively with two or more macromolecular targets, exerting their effects against certain therapeutic goals in a synergic fashion. This leading concept stimulated this COST Action focusing on novel ligands able to recognize selected multiple targets, to promote closer scientific links among European research groups involved in medicinal chemistry field at both academic and industrial level. The research competencies of the network will span around medicinal chemistry, from synthetic chemistry, natural products and biophysics to theoretical chemistry, molecular modelling and biological screening.
Project web page: http://www.mutalig.eu/
Duration: 4.12.2015 – 29.10.2020
GLUCOLIPOTOX – Ovplyvnenie molekulovych dráh glucolipotoxicity novým karboxymetylovaným merkaptotriazinoindolovým ihibítorom aldo-ketoreduktázy AKRlBl v diabete, zápale a vekom podmienenej neurodegeneráci
Targeting Molecular Pathways of Glucolipotoxicity by a Novel Carboxymethylated Mercaptotriazinoindole Inhibitor of Aldo-Keto Reductase AKR1B1 In Diabetes, Inflammation and Age-related Neurodegeneration
Program: Bilateral – other
Project leader: Ing. Štefek Milan, CSc.
Duration: 1.5.2016 – 30.4.2019
Dohoda o spolupráci medzi ÚNPF SAV a Ústavom základného psychologického výskumu a výskumných metód Fakulty psychológie Univerzity Viedeň
Program: Inter-institute agreement
Project leader: MUDr. Riečanský Igor, PhD.
Annotation:
Duration: 1.4.2015 – 31.12.2017
Evaluation of Quercetin and Green Tea in combination with Methotrexate for arthritis therapy (Acronym: PhytoArt 2.0)
Evaluation of Quercetin and Green Tea in combination with Methotrexate for arthritis therapy (Acronym: PhytoArt 2.0)
Program: Bilateral – other
Project leader: PharmDr. Bauerová Katarína, PhD., DrSc.
Duration: 1.1.2016 – 31.12.2017
Mechanizmy poškodenia srdca radiáciou a možnosti medikamentóznej prevencie.
Mechanisms of radiation injury to the heart. Preventive drug treatment.
Program: Multilateral – other
Project leader: D.h.c., Prof., MUDr. Slezák Ján, DrSc., FIACS
Duration: 1.1.2014 – 31.12.2017
Multidisciplinárna analýza kombinovaného vplyvu tyreoidnych hormonov a n-3 polynenasytených mastných kyselin u potkanov
Multidisciplinary analysis of the combined effects of thyroid hormones and n-3 polyunsaturated fatty acids in rats
Program: Inter-academic agreement
Project leader: RNDr. Tribulová Narcisa, DrSc.
Annotation: Thyroid hormones (TH) play a crucial role in healthy organisms, but profound alterations of their levels can affect metabolic and signaling processes in different tissues. We will use hyper- and hypothyroid rats as “model of a diseased organism” and analyze whether and how n-3 PUFA long term administration will ameliorate TH-induced pathophysiological changes. We will investigate skeletal and cardiac muscle tissue remodeling, changes in serum lipids and in body fat distribution, key enzymes of TH metabolism, oxidative stress in the whole organism and within the cell. We will focus on myosin heavy chain composition, distribution and phosphorylation of connexin-43, cellular calcium handling, membrane anisotropy, mitochondrial functions and related signaling pathways including cell death and antioxidant defense. As n-3 PUFA possess multiple sites of potential action, we believe that our complex research will contribute to a better understanding of molecular mechanisms governing n-3 PUFA actions and to their more effective application in the prevention of pathological symptoms in man.
Duration: 1.1.2015 – 31.12.2017
Odpovede krvného tlaku indukované stresom: endotelové faktory vs. centrálna regulácia sympatikového tonusu
Stress-induced pressor responses: endothelial factors vs. central regulation of sympathetic tone
Program: Inter-academic agreement
Project leader: RNDr. Bernátová Iveta, DrSc.
Annotation: Acute stress is considered to be a factor that may participate in stroke and heart attack. Mechanisms involved may be associated with inadequate acute stress-induced elevation of blood pressure (BP). On the basis of our previous joint research the aim of this project is to reveal differences in mechanisms involved in acute stress-induced pressor responses in adult normotensive and spontaneously hypertensive (SHR) rats. We will determine the role of nitric oxide (NO) in central regulation of sympathetic tone as well as the role of endothelium-derived factors (EDFs) such as NO, superoxide and hydrogen sulfide, in modulation of stress-induced BP responses. Acute stress will be induced by air-jet to the face of the rat. The effect of particular BP regulation systems will be investigated using inhibitors specific for the given system such as pentolinium (SNS), tempol (ROS), selective inhibitors of nNOS, iNOS and eNOS and other substances to inhibit selected EDFs. Resolving differences in acute stress-induced pressor responses could provide mechanism for selective prevention of dangerous stress-induced BP increase in hypertensive subjects and thus in prevention of stress-provoked stroke and heart attack.
Duration: 1.1.2016 – 31.12.2017
Sensorimotor – Stabilita postoja a trupu vo vzťahu k respiračným funkciám u zdravých jedincov a po transplantácii pľúc
Postural and core stability in association with respiratory functions in healthy and lung transplant individuals
Program: Bilateral – other
Project leader: Ing. Hlavačka František, CSc.
Annotation: Lung transplantation has been shown to be effective as a treatment for fatal and severecardiopulmonary diseases. In addition to survival, one goal of performing lung transplantation isimprovement in functioning and health. Several factors contribute to the maximal work capacityachievable after transplantation. Before surgery, patients are usually debilitated from the primarydisease and long-term inactivity, and the unavoidable complications of allograft transplantation,mainly lung resection, and toxic effects of immunosuppressant therapy contribute to the reducedfunctional capacity. While numerous investigators have described the features of the respiratoryand skeletal muscle function before and after surgery, little information is currently availableregarding the functional outcomes, such as strength and power, speed of step initiation, posturaland core stability, and so forth. It is mainly due to a lack of a standard evaluation system of theseabilities. Recently, we have developed new methodology for assessment of postural sway andbreathing movements of the chest using wireless accelerometers. Yet, it was not tested in lungtransplant patients and no investigations related to the relationship of variables of neuromuscularand cardiorespiratory functions were carried out. Therefore, we will assess postural and corestability, muscle strength and cardiorespiratory functions using posturography, surface EMG,accelerometry and clinical tests in healthy young and elderly individuals and lung transplantpatients. The findings will serve as a basis for better understanding of association betweenrelated variables in these people. As a result of the project will be elaborated methodology fortesting of neuromuscular and cardiorespiratory functions using novel techniques specificallydesigned for target population. Such objective and less time-consuming assessment can be wellfitted in an out-patients department and complement so existing diagnostic methods.
Duration: 1.1.2016 – 31.12.2017
Vplyv patologických stavov na odolnosť srdca voči ischémii myokardu: štúdium molekulárnych mechanizmov a nových možností kardioprotekcie
Effect of pathological states on cardiac resistance against myocardial ischemia: study of molecular mechanisms and novel approaches to cardioprotection
Program: Inter-academic agreement
Project leader: MUDr. Ravingerová Táňa, DrSc., FIACS
Annotation: Previously, we showed a negative impact of civilization diseases (hypertension, metabolic disorders) on adaptive mechanisms in the heart and its survival under conditions of acute ischemia/reperfusion (I/R). Comorbidities and age- and gender-related changes blunt protective cell signaling of ischemic preconditioning (PC) leading to acceleration of necrotic and apoptotic processes, impaired function of mitochondria and energy production. The project is focused on the study of molecular mechanisms that alter innate cardioprotection in the diseased myocardium and on possibilities to reactivate its adaptive potential. We will explore effects of novel forms of PC, so called „remote“ PC (induced by limb ischemia) that are less technically demanding and can be used in clinical practice, e.g., in elder patients with acute myocardial infarction. Investigation of mechanisms of „remote“ PC will enable its pharmacological simulation, e.g., using pleiotropic (other than primary) effects of PPAR agonists or their combination with PC that can facilitate stimulatory effect on cardiac resistance against I/R.
Duration: 1.1.2015 – 31.12.2017
BIOMAMI – Horčíkové nanokompozity pre biodegradovateľné medicínske implantáty
Magnesium Nanocomposites for Biodegradable Medical Implants
Program: Inter-academic agreement
Project leader: doc. RNDr. Pecháňová Oľga, DrSc.
Duration: 1.11.2014 – 31.10.2017

National

Systémový program pre komplexné poznanie a kritické myslenie formou zážitkového vzdelávania
Program: Other projects
Project leader: Ing. Ferko Miroslav, PhD.
Annotation:
Duration: 10.11.2021 – 31.3.2022
Deriváty kyseliny 1-indoloctovej ako inhibítory aldózareduktázy: vzťah štruktúry a aktivity
Indole-1-acetic acid derivatives as aldose reductase inhibitors: structure – activity relationships
Program: VEGA
Project leader: Ing. Šoltésová Prnová Marta, PhD.
Annotation:
Duration: 1.1.2018 – 31.12.2021
Elektrofyziologické koreláty a determinanty presnosti vizuálnej pracovnej pamäti
Electrophysiological correlates and determinants of visual working memory precision
Program: VEGA
Project leader: MUDr. Riečanský Igor, PhD.
Duration: 1.1.2019 – 31.12.2021
Mitochondrie ako kľúčový efektor v procesoch kardioprotektívnych intervencií
Mitochondria as a key effector in processes of cardioprotective intervention
Program: VEGA
Project leader: Ing. Ferko Miroslav, PhD.
Annotation:
Duration: 1.1.2018 – 31.12.2021
Nitrózo-sulfidová signálna dráha – nové regulačné vazoaktívne účinky v rôznych modeloch artériovej hypertenzie
Nitroso-sulphide signal pathway – novel regulator vasoactive effects in different types of arterial hypertension
Program: VEGA
Project leader: RNDr. Čačányiová Soňa, PhD.
Annotation: Nitric oxide (NO) and hydrogen sulphide (H2S) belong to important gaseous molecules engaged to the regulation of arterial tone in normotensive conditions. NO and H2S interaction includes a formation of new products which are part of an original nitroso-sulphide signalling pathway. Our previous experiments in Wistar rats demonstrated that these new signal molecules triggered a specific vasoactive response, different from the effect evoked by NO and H2S. In condition of arterial hypertension, the vasoactive effects of the novel signalisation have not been described yet. The aim of this project is to characterise the role of NO and H2S as well as of the nitroso-sulphide signalling pathway in different animal models of hypertension: essential (SHR), NO-deficient and also metabolic syndrome (hypertriglyceridemia – HTG). A simultaneous investigation of human vessels isolated from patients with hypertension and dyslipidemia represents an appropriate way how to associate results of basic research with clin. practise.
Duration: 1.1.2018 – 31.12.2021
Nové látky pre prevenciu a terapiu ochorení spôsobených toxicitou glukózy
Novel compounds in prevention and treatment of diseases caused by glucose toxicity
Program: VEGA
Project leader: RNDr. Májeková Magdaléna, PhD.
Annotation: In the long term lasting plasma glucose level may affect the course of physiological processes through numerousmetabolic pathways. A polyol pathway ranks among the important mechanisms of glucose toxicity and its mainenzyme – aldose reductase – is a frequent target in design of drugs reducing the progress of chronic diabeticcomplications. Another target could be introduced by calcium homeostasis in cells, as the cytosolic calcium levelis an important factor for many physiological processes, e.g. the insulin secretion. A modulation of calcium pumpSERCA becomes another mechanism for the manifestation of glucose toxicity. The aim of our project is to findnew compounds with polypharmacological effect, by means of combinatorial library of potential aldose reductaseinhibitors and actual knowledge on SERCA activity.
Duration: 1.1.2018 – 31.12.2021
Nové metódy prevencie a liečby oxidačného stresu, ischemicko-reperfúzne poškodenie a transplantácia srdca
Program: Other projects
Project leader: D.h.c., Prof., MUDr. Slezák Ján, DrSc., FIACS
Duration: 1.11.2019 – 31.12.2021
ACE2-TXZF – Nové perspektívy v liečbe kardiovaskulárnych komplikácií spojených s COVID-19
New perspectives in the treatment of cardiovascular complications associated with COVID-19
Program: SRDA
Project leader: RNDr. Čačányiová Soňa, PhD.
Annotation: Coronavirus disease 2019 (COVID-19), linked to severe acute respiratory syndrome induced by coronavirus-2(SARS-CoV-2), was declared as a global pandemic. While respiratory failure is the major cause of mortality due toCOVID-19, the great number of patients exhibit cardiovascular disorders. Understanding the underlyingmechanisms of cardiovascular complications associated with COVID-19 is of the great importance to reach theeffective therapy and to reduce mortality due to COVID-19. In this project we will imitate the inhibition of ACE2-mediated signalling induced by SARS-Cov-2 using highly specific ACE2 inhibitor MLN-4760. In thispharmacological model of COVID-19 in spontaneously hypertensive rats (SHR) we intend to examine the extend ofMLN-4760-induced vascular damage as well as the mechanisms underlying the action of taxifoline and zofenapril,as perspective pharmacological tools for the treatment of cardiovascular complications associated with COVID-19.TX has been chosen as it was shown as promising drug-like substance inhibiting SARS-Cov-2 replication viainhibition of the main protease (Mpro). ZF is sulfhydryl-containing angiotensin converting enzyme (ACE) inhibitorspontaneously releasing hydrogen sulfide (H2S). Both substances provide several additional cardioprotectiveeffects associated with elevated NO bioavailability and H2S release, respectively. These effects can improvefunction of the cardiovascular system via elevation of NO and H2S-mediated vasodilatation, inhibition oftrombogenesis and induction of antioxidant and antiinflamatory action.
Duration: 16.9.2020 – 31.12.2021
Nové prístupy k liečbe kachexie, zápalu a oxidačného stresu v experimentálnej artritíde: Účinok rôznych rastlinných extraktov z olivových listov, Rhodiola rosea, Tribulus terrestris a extra panenského olivového oleja
Program: VEGA
Project leader: PharmDr. Poništ Silvester, PhD.
Annotation:
Duration: 1.1.2019 – 31.12.2021
Ochrana srdca v situáciách nadmernej tvorby kyslikových a nitrozylových radikálov: Molekulárny vodík ako nový potenciálny therapeutický nástroj?
Heart protection in situations of excessive formation of oxygen and nitrosyl radicals: Molecular hydrogen as a new potential therapeutic tool?
Program: VEGA
Project leader: RNDr. Kura Branislav, PhD.
Annotation:
Project web page: https://evega.minedu.sk/e-vega/(S(yi1zctb4lxjmuf55ntk1jfbm))/default.aspx
Duration: 1.1.2018 – 31.12.2021
Štúdium spúšťacích faktorov a mechanizmov prenosu signálu indukovaných neinvazívnymi adaptačnými intervenciami v organizme potkana za účelom ochrany myokardu pred schémiou
Study of triggering factors and signal transduction mechanisms induced by noninvasive adaptive interventions in rats aimed to protect myocardium against schemia
Program: VEGA
Project leader: MUDr. Ravingerová Táňa, DrSc., FIACS
Annotation: Cardiovascular diseases are one of the leading causes of mortality in modern society. They are predicted to rise over the coming decades, due to aging population, longer survival of patients after myocardial infarction, and incidence of civilization diseases. Research pointed out to the protective effects of adaptive phenomenon of ischemic preconditioning (IPC) and its novel clinically acceptable and safer forms. Currently, cellular mechanisms activated by stimuli like exercise, acute hypoxia and PC of the remote organ are not yet completely elucidated as compared with classiccal IPC. For that reason, several pathological animal models (myocardial ischemia, hypertension, d. mellitus, dyslipidemia) will be used. Acute and longer lasting adaptive interventions will be tested using relevant methodology (combination of physiological, morphological and biochemical techniques). The results obtained in this project may lead to development of novel or modified therapeutic strategies to manage myocardial ischemia
Duration: 1.1.2018 – 31.12.2021
Vplyv virtuálnej reality na senzorickú reguláciu rovnováhy, fyziologické a psychologické funkcie človeka
The effect of virtual reality on the sensory regulation of balance control, physiological and psychological functions in humans
Program: VEGA
Project leader: Mgr. Hirjaková Zuzana, PhD.
Annotation: Virtual reality (VR) is an environment that can be used to assess the postural and psycho-physiologicalparameters of different groups of people. The virtual environment provides an experience close to reality inlaboratory conditions or at home. The aim of the project is to gain new knowledge about the body orientation,sensory regulation of balance control and the level of psycho-physiological stimulation in the VR. The volunteerswill be introduced into the VR and at the same time their postural activity, reactions to vibratory stimulation oflower limb muscles, physiological and psychological level of arousal and stress will be recorded. We assume thatthe project results will provide the basis for designing promising methods for testing sensory balance control andpsycho-physiological reactivity to the virtual environment. The obtained results may be helpful in rehabilitation ofpatients with postural balance disorders as well as people with psychological or physiological difficulties.
Duration: 1.1.2019 – 31.12.2021
RIDD – Výskum magnetických foriem železa v rozvoji kardiovaskulárnych chorôb a porúch správania
Research of magnetic forms of iron in development of cardiovascular diseases and behavioural disorders
Program: SRDA
Project leader: RNDr. Bernátová Iveta, DrSc.
Annotation: This project proposal is focused on the investigation of the role of iron in development of cardiovascular and behavioural disorders, prevalence of which is increasing during aging. The aim of this project is to investigate theimpact of aging on the metabolism of biogenic iron and its magnetic properties in association with metabolic and functional alterations in the cardiovascular system and brain in rats with various genetic predispositions tohypertension. We will determine the molecular biological changes on the level of gene expression, their encoded proteins and the activities of the enzymes involved in the endogenous antioxidant protection, the regulation of nitric oxide production and cell death due to ferroptosis in course of aging. We will also investigate the impact ofexogenously administered iron in the form of the biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) on blood pressure regulation and function of the heart and blood vessels in conditions of normotension, chronically increased blood pressure and acute stress (i.e. acutely elevated blood pressure).Results achieved in this project will contribute to better understanding of the effects of the altered iron metabolism, magnetic forms of bodily iron, as well as iron in the form of USPIONs, on the cardiovascular andcentral nervous systems and to prevention of cardiovascular risk resulting from the use of USPIONs in targeted drug delivery or as the contrast materials for new imaging methods in medicine.
Project web page: http://www.bionanoiron.sav.sk
Duration: 1.7.2017 – 30.6.2021
Interakcia metabolických faktorov a neurogénnej signalizácie pri experimentálnych modeloch depresie
Crosstalk of metabolic factors and neurogenic signaling in experimental models of depression
Program: VEGA
Project leader: RNDr. Vranková Stanislava, PhD.
Annotation:
Duration: 1.1.2018 – 31.12.2020
Mechanizmy zahrnuté v endotelovej dysfunkcii indukovanej kyselinou močovou v závislosti od veku a genetickej predispozície k hypertenzii
Mechanisms involved in uric acid-induced endothelial dysfunction depending on the age and genetic predisposition to hypertension
Program: VEGA
Project leader: MUDr. RNDr. Púzserová Angelika, PhD.
Annotation: Studies have shown a significant relationship between increased concentration of uric acid in the blood (hyperuricaemia) and cardiovascular diseases, including hypertension. But there is little information on the mechanisms by which uric acid can lead to end-organ damage. Hyperuricaemia combined with hypertension is associated with endothelial dysfunction. However, the mechanisms by which hyperuricaemia causes endothelial dysfunction are not clarified. This project aims to clarify the relationship of hyperuricaemia and hypertension, especially in terms of endothelial function. The aim is to bring new results highlighting the impact of elevated concentrations of uric acid on the endothelium, and to reveal the mechanisms involved in endothelial dysfunction in conductive and resistant arteries isolated from peri-pubertal and adult normotensive, prehypertensive and hypertensive rats. The results will contribute to the knowledge about pathophysiology of hyperuricaemia-induced endothelial dysfunction.
Duration: 1.1.2017 – 31.12.2020
Redoxná homeostáza, proteostáza a zápal ako potenciálne ciele pre ovplyvnenie starnutia a s ním spojených ochorení: Modulácia pomocou látok prírodného a syntetického pôvodu
Redox Homeostasis, Proteostasis and Inflammation as Potential Targets For Influencing Ageing and Age-Related Diseases: Modulation by the compounds of natural and synthetic origin
Program: VEGA
Project leader: Ing. Račková Lucia, PhD.
Annotation: Ageing is a natural and inevitable phenomenon which is also the main risk factor for the serious diseases. Free-radical theory of ageing states that organisms age because cells accumulate free radical damage over time. These changes are significantly amplified by the decline in proteolytic capacity. Microglia, as the main immune effector cells of the CNS, undergo these alterations as well. This results in their chronic activation and increased risk of neurodegeneration. The aim of the project is to investigate the potential of compounds of natural and synthetic origin to up-regulate Nrf-2/Keap-1 signalling pathway (in particular, the activation of genes responsible for maintenance of redox homeostasis and proteostasis), also in view of their simultaneous down-regulation of NFkB in microglia. Subject of the project will be also investigation of postranslantional modifications in the regulatory mechanisms of proteolysis in microglial cells.
Duration: 1.1.2017 – 31.12.2020
Úloha Nrf2 signálnej dráhy v odpovediach srdcových buniek na patologické podnety
Role of Nrf2 signaling pathway in responses of cardiac cells to pathological conditions
Program: VEGA
Project leader: RNDr. Barančík Miroslav, DrSc.
Duration: 1.1.2018 – 31.12.2020
Vlastnosti Na,K-ATPázy, jedného z kľúčových systémov pre udržiavanie koncentrácie sodíka v organizme, v podmienkach zaťaženia organizmu po ožiarení.
Properties of the Na,K-ATPase, representing one of the crucial systems in maintaining the sodium homeostasis in the organism, after irradiation.
Program: VEGA
Project leader: RNDr. Vrbjar Norbert, CSc.
Annotation: The present project is oriented to obtain new data concerning the maintenance of intracellular homeostasis of sodium, representing one of the unavoidable factors for appropriate regulation of cellular viability, after application of radiotherapy. Using in vivo model (rat) we will investigate the influence of gamma irradiation on the cerebral and renal Na,K-ATPase which is one of the crucial systems in maintaining appropriate intracellular concentration of sodium ions. The data will contribute to elucidation of molecular background of processes involved inmaintaining the cell’s viability in kidney and in brain from the aspect of possible protection of the organism against deleterious side-effects of radiotherapy.
Duration: 1.1.2017 – 31.12.2020
Vplyv prenatálnej hypoxie na vývin jedinca a možnosti terapie jej dlhodobých dôsledkov
Program: Other projects
Project leader: PharmDr. Piešová Michaela
Duration: 1.1.2020 – 31.12.2020
Vplyv ultra malých superparamagnetických nanočastíc železa na kardiovaskulárny systém potkana v podmienkach vysokého krvného tlaku
Effect of ultrasmall superparamagnetic iron oxide nanoparticles on the cardiovascular system of rats with high blood pressure
Program: VEGA
Project leader: RNDr. Bernátová Iveta, DrSc.
Annotation: This project will investigate the effect of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) on function and structure of the arterial wall and the heart in rats with high blood pressure. We will investigate if acute stress and chronic high blood pressure can facilitate the USPIONs uptake in the arterial wall and heart, tomodify cardiovascular function, including blood pressure regulation and to induce metabolic disorders, oxidative damage and alterations of the Fe2+/Fe3+ ratio in the heart and vasculature. We will investigate if L-type ofvoltage-dependent calcium channels is involved in iron uptake after USPIONs treatment. Results achieved in this project will contribute to better understanding of USPIONs effects on the cardiovascular system in conditions ofacute stress and high blood pressure as well as on prevention of cardiovascular risk resulting from the use of USPIONs in targeted drug delivery.
Duration: 1.1.2017 – 31.12.2020
Výskum možností a rozvoj SQUID magnetometrie pre vybrané aplikácie v biomedicíne a materiálovom výskume
Research on possibilities and development of SQUID magnetometry for selected applications in biomedicine and material research
Program: VEGA
Project leader: RNDr. Bernátová Iveta, DrSc.
Annotation: Project has an interdisciplinary character.The aim is to show the possibilities of use of the SQUID magnetometry in study of the actual processes in medicine, biology and material research:-in analysis of the properties and magnetic characterization of the nanoparticles and nanoliquids, especiallyultra-small superparamagnetic nanoparticles based on iron oxides (USPIONs)-in investigation of the influence of the USPIONs on the function and structure of the blood vessels and heart, on development of the oxidative damage and in study of processes of the USPIONs transport through cell membranes, blood vessels and organs of rats with normal and high blood pressure-in development of the procedures and methods of quantification of the magnetic substances content in thehuman and animal cell cultures and organs- in study and development of the aluminate glasses with photoluminescence properties and other applications.
Duration: 1.1.2017 – 31.12.2020
Vzťah medzi telesnou adipozitou a funkčnými vlastnosťami artérií u potkana
Relationship between body adiposity and functional properties of arteries in rat
Program: VEGA
Project leader: Mgr. Zemančíková Anna, PhD.
Annotation: High levels of body mass index are considered as an important risk factor contributing to cardiovascular impairment. However, recent studies have brought some noteworthy findings that moderately increased amount of body fat is beneficial with respect to prognosis of patients with heart and vessel diseases. The aim of this project is to analyse the relationship between the level of body adiposity/weight and cardiovascular function in terms of blood pressure regulation and functional properties of selected arteries in rat. An effort will be made to reveal how the vessels are modulated by the adjacent perivascular adipose tissue (PVAT) in healthy normotensive and in hypertensive rats after moderate and considerable increase in body adiposity induced by dietetic interventions in different ontogenetic stages. Several biometric and hemodynamic parameters will be measured, as well as some biochemical parameters in blood and tissues and in vitro reactivity of arteries in the presence of PVAT and after its removal.
Duration: 1.1.2018 – 31.12.2020
DYSRPONEP – Dynamika srdcového poškodenia: úloha nekroptotickej bunkovej smrti a prežívania kardiomyocytov.
Dynamics of myocardial damage: a role of necroptotic cell death and survival of cardiomyocytes.
Program: SRDA
Project leader: MUDr. Ravingerová Táňa, DrSc., FIACS
Annotation: Cardiomyocytes have a limited capability to proliferate, self-renew and repair. Therefore, the number of cardiac cells during postnatal development determines how the heart can deal with stress and workload demands. Both sudden acute or chronic progressive loss of heart cells are involved in the development of ventricular dysfunction and the progression of heart failure (HF). In cardiomyocyte loss, apoptosis seems to play a minor role comparedto necrosis. In line with this, we have found that markers of a newly described form of regulated necrosis – necroptosis are increased in human failing hearts whereas apoptotic ones are unchanged. Although it has been proposed that these markers form a cytotoxic complex, it is unclear how it induces necroptotic cell death. In this process, a protein RIP1 plays an important role. However, when it is polyubiquitinated it may trigger pro-survivalpathways instead. Stimuli, which preferentially trigger RIP1-mediated cell survival at the expense of cell death, are mostly unknown. In this project, we will study the cytotoxic action of necroptotic complexes with respect totranslocation into cell structures, disturbed ion homeostasis and oxidative stress. In addition, we will study whichRIP1-associated signaling is triggered during cell survival (eg. ischemic preconditioning – IPC) and which RIP1 pathways lead to the contrary, death of cardiac cells in acute myocardial infarction and during the development of HF of various origin. By using a necroptosis inhibitor, we will try to determine whether it affects IPC-induced cardioprotection with respect to cell viability and whether its time-dependent application can relieve remodeling and cardiac dysfunction due to myocardial infarction or induce regression of damage. Cardiomoycytes, isolated hearts and in vivo studies will be used to investigate signaling pathways and autocrine/paracrine and systemwide responses to a primary impulz that induces the damage/ adaptation of the heart.
Duration: 1.7.2016 – 30.6.2020
G-LUCK – Farmakologické ovplyvnenie glukózovej toxicity pri diabete typu 2
Pharmacological intervention in glucose-toxicity in type 2 diabetes
Program: SRDA
Project leader: RNDr. Májeková Magdaléna, PhD.
Annotation: Glucose toxicity plays important role not only in a development of chronic diabetic complications but also in aninduction of insulin secretion disorder, decrease of beta cell mass and development of insulin resistance. Theaim of this project is to design and test novel compounds with indolyl acetic acid scaffold, multi-target-directedligands, which should slow down the onset of diabetes mellitus type 2 and reduce progression of diabeticcomplications.
Duration: 1.7.2016 – 30.6.2020
MEDMEX – Kompenzačné ochranné mechanizmy ako účinný nástroj voči zvýšenej energetickej deficiencii patologicky zaťaženého myokardu: Výhodná perspektíva v modernej experimentálnej kardioprotekcii.
Study of endogenous compensatory mechanisms effective against energy deficiency in pathologycally loaded myocardium: Innovative approaches in experimental cardioprotection.
Program: SRDA
Project leader: Ing. Ferko Miroslav, PhD.
Annotation: Research on field of compensatory mechanisms appears to be promising method for endogenous protection inpathologically loaded myocardium under condition of increased energy demands. The project aims to contributeto the knowledge in the field of experimental cardiology and point to new, alternative cardioprotective proceduresagainst ischemia-reperfusion injury. It is necessary to study the protective signaling pathways, propose potentialcardiomarkers and identify positive functional changes observed at the level of cardiac mitochondria and heart itsself for comprehensive understanding of the onset and progression of mechanisms of endogenous myocardialprotection leading to effective compensation against energy deficiency in ischemia/reperfusion injury. Severalapproaches utilize processes of endogenous protection to achieve cardioprotection, such as ischemic andpharmacological preconditioning, clinically applicable „remote“ ischemic preconditioning (RIP) as well asexperimental streptozotocine-induced diabetes mellitus in acute state. Coexistence of several comorbidities (hypercholesterolaemia, hypertension) suppress mechanisms of cell signaling involved in protective effect ofischemic preconditioning that promotes necrotic and apoptotic processes in the myocytes during ischemiareperfusionchallenge and also reduces energy production. With respect to bioenergetics and the role ofmitochondria involved in the execution phase of cardioprotection as a common mechanism in various types ofadaptive phenomena, the information is scarse up to date. It is expected that the project will help to characterizethe changes caused by functional remodeling of the mitochondrial membrane, and provide a new and currentlyabsenting information on regulation of mechanisms against increased enegetic demands resulting in myocardialsurvival.
Duration: 1.7.2016 – 30.6.2020
Molekulárno-farmakologické prístupy k inovatívnej terapii reumatoidnej artritídy hodnotenej v experimentálnych podmienkach in vivo a in vitro
Program: SRDA
Project leader: PharmDr. Bauerová Katarína, PhD., DrSc.
Duration: 1.7.2016 – 30.6.2020
NO-NEW-REG – Nové regulačné účinky oxidu dusnatého a ich úloha v rozvoji esenciálnej hypertenzie
New regulatory effects of nitric oxide and their role in the development of essential hypertension
Program: SRDA
Project leader: RNDr. Čačányiová Soňa, PhD.
Annotation: High blood pressure is a main risk factor in sustained increased morbidity and mortality of humans suffering cardiovascular diseases. Understanding of causes leading to hypertension enable to reveal new preventive andtherapeutic decisions. A new regulatory system involved in vessel tree regulation seems to be neuronal NOsynthase (nNOS) and its interactions with other regulatory systems. On the level of the kidney nNOS signal pathway interferes with renin-angiotensin system (RAS) and sulfide signalization (H2S), and the interactionsamong them are the unexplored area of regulatory mechanisms. nNOS in macula densa stimulate renin syntesis and via it influences RAS and sympathetic nerve system, on the other hand, H2S inhibits renin synthesis. Moreover, the regulatory pathways of nNOS and also endothelial eNOS could interact with endogenous NOSinhibitor, asymmetric dimetylarginine ADMA, on local as well as systemic level. The aim of the project is to study the effect of interactions of NO/nNOS/eNOS signalization with mentioned regulatory pathways (RAS, H2S,ADMA) on cardiovascular system and to find out their role in the specificity of nNOS and/or eNOS action in the conditions of essential hypertension. The availability of the results will be reached via using complex approach(functional, molecular, morphological). Moreover, on the level of acute experiments, we will confront selectedbiochemical markers of perivascular adipose tissue (plasma/serum) as well as vasoactive responses of arteriesisolated from normotensive and hypertensive rats with biochemical markers and reactivity of vessels isolated after nephrectomy from normotensive patients and patients with essential hypertension.
Duration: 1.7.2016 – 30.6.2020
SCAVRAD – Ochrana srdca v situáciách zvýšenej produkcie voľných kyslíkových radikálov: Radiačné a reperfúzne poškodenie.
Protection of the heart in situations of increased production of oxygen free radicals: Radiation and reperfusion injury.
Program: SRDA
Project leader: D.h.c., Prof., MUDr. Slezák Ján, DrSc., FIACS
Annotation: According to statistics, cardiovascular and cancer are the main cause of more than 93% of the global morbidityand mortality.One of the most used methods to treat patients with cancer is radiotherapy, which uses ionizing radiation.Ionizing radiation damages the cancer cells, leading to their apoptosis and to potential patient recovery.However, during irradiation of cancer cells may also occur unintended exposure of surrounding healthy tissue,which in turn can cause serious health complications including radiation-induced heart disease. Ionising radiationacts directly on the DNA of cells, or indirectly through the formation of free radicals, which then damage theindividual organelles of cells or DNA. Production of oxygen free radicals, which in addition have a signalingfunction, at higher concentrations have toxic effects on all parts of the heart and blood vessels, is a commondenominator of both ionizing radiation and inflammation, as well as ischemic and reperfusion injury. Therefore,research in this field, and finding novel suitable materials which can positively influence the effects of over
Duration: 1.7.2016 – 30.6.2020
Prenatálne a postnatálne účinky ligandov δ a µ opioidných receptorov na vývoj a funkciu hipokampu
Prenatal and postnatal effects of δ and µ opioid receptor ligands on the hippocampal development and function
Program: SRDA
Project leader: RNDr. Dubovický Michal, CSc.
Annotation: Ligands of opioid receptors δ (DOR) and µ opioid receptors (MOR) are commonly used in treatment of severe acute and chronic pain. DORs are involved also in mood disorders like depression and anxiety, which are related to the hippocampal function. Treatment with DOR ligands does not result in adverse effects including addiction, which are common with MOR ligands. However, much less is known about DOR – activated signaling pathways than about MOR – activated pathways. We will analyze the effect of acute (seconds to minutes) and chronic (hours to days) in in vitro and in vivo (prenatal and postnatal) administration of DOR ligands on the morphological and electrophysiological properties of rat hippocampal neurons and compare them with effects of MOR ligands and with effects of ligands specific for MOR-DOR heteromers. Further, involvement of calcium transporting proteins in signal transduction pathways activated by DORs and MORs ligands will be addressed by molecular biology methods. Possible remodeling of the dendritic spines will be investigated using transmission electron microscopy. Effect of DOR ligands on hippocampal plasticity in control and stressed rats will be examined using behavioral tests and molecular neuroscience techniques. Excitability will be investigated in primary culture of hippocampal neurons by patch clamp and in situ by in vivo electrophysiology. Both models enable to follow effects of acute and chronic drug application as well as possible receptor desensitization and offer complementary advantages. Primary neuronal culture is the possibility to visually identify neurons, characterize in details both action potentials and underlying ionic currents and to correlate electrophysiology and molecular biology on the same batch of neurons. In vivo electrophysiology offers the possibility to measure neuronal activity within its normal environment including all interactions with other brain parts.
Duration: 1.7.2016 – 30.6.2020
Štúdium anatomicko-funkčných rozdielov v účinkoch aripiprazolu a kvetiapínu, atypických antipsychotík s podobnými terapeutickými vlastnosťami, ale rozdielnym vplyvom na dopaminergické receptory v mozgu, u experimentálnych zvierat
Investigation of anatomical-functional differences between the effects of aripiprazole and quetiapine, atypical antipsychotics with similar therapeutic indications, but different impact on brain dopaminergic receptors, in experimental animals
Program: SRDA
Project leader: RNDr. Mach Mojmír, PhD.
Annotation: Antipsychotics (APs) represent a group of drugs used in the treatment of spectrum of psychotic and depressive disorders. However, frequency of ATs treatment is rather increasing than decreasing and growing number of atypical AP drugs have also been emerged over the last few years. In addition, APs treatment is connected with a number of unwanted side effects, such as extrapyramidal syndrome, akathisia, body mass increase, agranulocytosis, tardive dyskinesia, somnolencia, etc. Anatomical-functional investigations are incessantly bringing information about the effects of APs on the activity of neurons and their spatial distribution in the brain, which allows more precisely to define and predict the consequences of the APs treatment. The aim of the present study is to reveal the effect of acute and repeated treatment of two, relatively new atypical APs, aripiprazole (ARI) and quetiapine (QUE), on the activity of neurons in the forebrain and extra forebrain areas of the brain, to identify the phenotype (chemical) character of the targeted neurons, to investigate their impact on the behavior and to compare their impact on the activity of signaling pathways, expression of signaling molecules, and secretion of selected neuropeptides in anatomically precisely defined brain structures. The data of the present project will be new and will serve for the deeper understanding of the biology of serious mental disorders. They also may bring new impulses to the drug developing processing to prepare drugs with more directed and beneficial therapeutic features.
Duration: 1.7.2016 – 30.6.2020
H2S-NO – Štúdium biologických účinkov produktov H2S/NO interakcie a molekulárne mechanizmy ich pôsobenia
Study of biological effects of H2S/NO products and molecular mechanism of their actions
Program: SRDA
Project leader: RNDr. Čačányiová Soňa, PhD.
Annotation: Now it is well acknowledged that endogenously produced H2S affects and is involved in regulation of many physiological and pathological functions of living organisms. It is suggested that biological effects of H2S mightnot result from actions of H2S alone, but from its oxidation products, which come from e.g. interaction of H2S with NO. Last four years, our “international” group indentified the following products of H2S and NO interaction:nitrosopersulfide (SSNO−), polysulfides (HSn−) and dinitrososulfite [N-nitrosohydroxylamine-N-sulfonate (SULFI/NO) (Proc Natl Acad Sci U S A. 112, 2015, E4651-E4660). Biological effects and molecular mechanisms of these products are not completely understood Therefore the aim of our project is to explore biological effectsof the products of H2S/NO interactions and to study their molecular mechanism of their actions. Particularly, as a continuation of our research, we will study their effects on rat blood pressure and aortic rings relaxation. Toelucidate molecular mechanisms of their biological effects, we will study their influence on expression ofenzymes that endogenously produce H2S (CBS, CSE and 3-MST), on intracellular membrane channels,concentration of intracellular calcium, lipid peroxidation and their antioxidant properties. Goal of the project isalso to find out, if studied compounds could provide us with information leading to a drug design based on their molecular structure, what could be an object for next application studies and lead to implementation in medical praxis.
Duration: 1.7.2016 – 30.6.2020
MuTaLig – MVTS- Viac-cieľový model pre inovatívnu identifikáciu látok v procese objavovania liečiv
Multi-target paradigm for innovative ligand identification in the drug discovery process
Program: Vedecko-technické projekty
Project leader: RNDr. Májeková Magdaléna, PhD.
Annotation: The aim of this COST Action is to join highly-qualified research teams working in disciplines around the field of medicinal chemistry, into a novel network devoted to the multi-target issue in drug discovery. The choice of this theme is related to its marked multidisciplinary character, which can ensure a strong interaction among all COST Action participants. Currently, an important and emerging issue in modern drug discovery is to design novel or identify existing bioactive compounds, endowed with the capability to interact selectively with two or more macromolecular targets, exerting their effects against certain therapeutic goals in a synergic fashion. This leading concept stimulated this COST Action focusing on novel ligands able to recognize selected multiple targets, to promote closer scientific links among European research groups involved in medicinal chemistry field at both academic and industrial level. The research competencies of the network will span around medicinal chemistry, from synthetic chemistry, natural products and biophysics to theoretical chemistry, molecular modelling and biological screening.
Duration: 18.4.2016 – 17.4.2020
Hypoxia ako prevencia zlyhávania srdca potkana a jej vplyv v rôznych fázach zlyhávania: Charakteristika funkčných, štrukturálnych a molekulárnych zmien.
Hypoxia in the prevention of heart failure in rats and its influence in various stages of heart failure: Characteristics of functional, structural and molecular changes.
Program: VEGA
Project leader: Mgr. Farkašová Veronika, PhD
Duration: 1.1.2017 – 31.12.2019
RADISCAV – Nové metódy pre zlepšenie diagnostiky, prevencie a liečby kardiovaskulárnych ochorení so zameraním na oxidačný stres. Ochrana pred radiáciou indukovaným poškodením srdca. Reperfúzne poškodenie – transplantácia srdca
New methods to improve diagnostics, prevention, and treatment of cardiovascular diseases with focus on oxidative stress. Protection from radiation-induced heart damage. Reperfusion injury – heart transplantation
Program: Other projects
Project leader: D.h.c., Prof., MUDr. Slezák Ján, DrSc., FIACS
Annotation:
Project web page: https://stimuly.vedatechnika.sk/
Duration: 1.12.2018 – 31.12.2019
Nové prístupy k liečbe kachexie, zápalu a oxidačného stresu v experimentálnej artritíde: Účinok rôznych rastlinných extraktov z olivových listov, Rhodiola rosea, Tribulus terrestris a extra panenského olivového oleja
Program: VEGA
Project leader: PharmDr. Poništ Silvester, PhD.
Duration: 1.1.2019 – 31.12.2019
Ochrana mechanizmov modulujúcich permeabilitu endotelu v srdci.
Protection of mechanisms modulating endothelial permeability in the heart.
Program: VEGA
Project leader: RNDr. Okruhlicová Ľudmila, CSc.
Duration: 1.1.2016 – 31.12.2019
Prenatálne programovanie chorôb v dospelosti: subchronická prenatálna asfyxia u potkanov ako vhodný model na štúdium mechanizmov embryo-fetálneho programovania neurobehaviorálnych zmien v dospelosti
Prenatal programming of psychiatric diseases: experimental approaches for evaluation of causes and mechanisms of their origin
Program: VEGA
Project leader: RNDr. Mach Mojmír, PhD.
Annotation: There are many evidences on neurodevelopmental origin of mental diseases. Various environmental and maternal factors acting during prenatal period and early childhood can increase sensitivity of the individual to anxiety, depression or other mental disorders in later postnatal life. Insufficient oxygen and nutrition supply of tissues, excessive stress or chemical substances and drugs can adversely affect the development of the brain. The mechanisms and causes of prenatal programming will be studied using animal model of chronic prenatal asphyxia in rats to uncover basis for the increased sensitivity of the organism to the emergence and development of neurobehavioral and cardiovascular disorders in adulthood. Early detection of these mechanisms on the basis of molecular, biochemical and behavioral indicators can contribute to therapeutic interventions in the early stages of development and thus reduce or eliminate the occurrence of these diseases in later life.
Duration: 1.1.2016 – 31.12.2019
Prevencia hypoxicko-ischemického poškodenia neonatálneho mozgu potkana: testovanie nových spôsobov farmakologickej a nefarmakologickej intervencie
Prevention of hypoxic-ischemic damage of the neonatal rat brain: testing of novel approaches involving pharmacological and non-pharmacological intervention
Program: VEGA
Project leader: RNDr. Juránek Ivo, PhD., DrSc.
Annotation:
Duration: 1.1.2016 – 31.12.2019
Relevancia nekroptózy v odumieraní myokardiálneho tkaniva v dôsledku rôznych typov poškodenia: vplyv na excitačno-kontrakčné prepojenie.
Program: VEGA
Project leader: Ing. Ferko Miroslav, PhD.
Duration: 1.1.2016 – 31.12.2019
Skúmanie regulačných mechanizmov medzibunkovej komunikácie v srdci pre cielenú ochranu pred jeho funkčným zlyhaním.
Investigation of regulatory mechanisms of cardiac cell-cell communication for targeted protection from heart failure.
Program: VEGA
Project leader: RNDr. Tribulová Narcisa, DrSc.
Annotation: Cardiac cell-to-cell communication via gap junction connexin (Cx) channels is essential for synchronised heart function. While disorders in expression, distribution and phosphorylation of Cx in of both human and animal heart diseases promote occurrence of malignant arrhythmias and heart failure. We hypothesize that targetedmodulation of Cx channel function by exogenous and endogenous compounds may be a promising approach to protect proper heart function. Aim of this project is to elucidate mechanisms implicated in regulation ofCx43-mediated cardiac cell-to-cell communication in healthy and diseased heart. Research findings should enhance knowledge of cardiologists in this field and challenge the realization of clinical trials supporting novel approaches in prevention and/or treatment of heart diseases to fight sudden cardiac death.
Duration: 1.1.2016 – 31.12.2019
ENDBIOM – Sledovanie kritických endogénnych biomarkerov a signálnych dráh v hypertenzii a pri kardiovaskulárnych ochoreniach
Study of critical endogenous biomarkers and signaling pathways in hypertension and cardiovascular diseases
Program: VEGA
Project leader: doc. RNDr. Dovinová Ima, PhD.
Annotation: Several endogenous factors and signaling pathways contribute to the development of cardiovascular diseases. Activation of the renin-angiotensin and aldosterone system (RAAS), deteriorated relaxation of vasoactive systems producing NO and H2S, as well as an imbalance of redox pathways producing reactive oxygen and nitrogen species (ROS a RNS). Hypertension is a major risk factors of cardiovascular diseases and leads to functional and structural alterations in blood vessel walls. The landmarks of developed hypertension are increased vasoconstriction regulated by RAAS, oxidative stress manifested in increased ROS and RNS, endothelial dysfunction (increased levels of assymetric dimethyl-arginine – ADMA – and homocystein) as well as cardiovascular remodeling (activation of metaloproteinases). Detection, monitoring, and regulation of the critical pathology markers can lead to a better management of hypertension- and metabolic syndrome-related diseases.
Duration: 1.1.2017 – 31.12.2019
Špecifické metódy a inovované postupy posudzovania výkonnosti športovcov a telesnej zdatnosti bežnej populácie
Specific methods and innovative procedures for assessing performance in athletes and physical fitness in the general population
Program: VEGA
Project leader: RNDr. Bzdúšková Diana, PhD.
Annotation: The project will design specific tests and innovative methodological procedures for assessing performance inathletes and physical fitness in the general population. These will be based on analysis of performance inselected sports and age specificities of the general population. Methodology of measurement and data analysiswith the highest accuracy of motor abilities evaluation will be verified. Their ability to differentiate between andwithin groups differences will also be evaluated. Part of the project will be testing guidance scales on groups ofelite athletes of selected sports and large population of varied ages. This will provide a basis for testing protocolsand recommendations for utilization of novel diagnostic methods in practice. Such diagnostics taking into accountspecific conditions of particular sports and age specificities represent significant shift in obtaining relevantinformation on performance in athletes and physical fitness in the general population and their longitudinalchanges.
Duration: 1.1.2017 – 31.12.2019
Účinok STAT1 a ISG15 inhibítorov na biochemické a morfologické parametre pri experimentálnom infarkte myokardu
The effect of STAT1 and ISG15 inhibitors on biochemical and morphological parameters in experimental myocardial infarction
Program: VEGA
Project leader: RNDr. Cebová Martina, PhD.
Annotation: Myocardial infarction (MI) remains a major cause of morbidity and mortality throughout the world. Atherosclerosis,chronic inflammation and metabolic diseases are the main causes of MI. Hypertension is the most serious riskfactors which worsen the prognosis of MI via induction of oxidative and inflammatory mediators. Chronic ischemialeads to irreversible myocardial damage. Reperfusion of myocardium may potentially save myocardial function,paradoxically, this process causes further damage of the myocardium and apoptosis of cardiomyocytes alongwith upregulation of STAT1 and ISG15. Thus, it is important to study different molecules which may block orrevers pathological processes in myocardial reperfusion injury following MI at different levels. Therefore, the aimof our study is to analyze the effects of STAT1 and ISG15 inhibitors on reperfusion injury in the experimentalmodel of MI (ischemia-reperfusion injury after STEMI revascularization) and determination of pathophysiologicalchanges in this process.
Duration: 1.1.2017 – 31.12.2019
Úloha extracelulárnych vezikúl v medziorgánovej komunikácii zahrnutej v kardioprotekcii na diaľku (remote conditioning).
The role of extracellular vesicles in inter-organ communication related to remote cardioprotection
Program: VEGA
Project leader: doc. RNDr. Barteková Monika, PhD.
Annotation: Inter-organ communication plays a crucial role in cardioprotection induced by an ischemic (or other) insult on remote organ to the heart, called remote ischemic preconditioning, or remote conditioning in general. Extracellular vesicles (EVs) are membrane-bound structures secreted by a wide range of mammalian cell types that can be secreted and specifically taken up by other cells. Since EVs contain a high concentration of RNAs and proteins, they are of a high interest as potential mediators of remote cardioprotection, and thus for inter-organ signal transfer mechanisms in general. Revealing the role of EVs in communication between different cells and organs as well as identifying substances transported by EVs to be potential mediators of cardioprotection as the main goal of the current project could lead to better understanding of remote cardioprotection and inter-organ communication in general, and rise up new potential targets of therapy of heart ischemia.
Duration: 1.1.2016 – 31.12.2019
Vplyv transkraniálnej stimulácie mozgu jednosmerným prúdom na senzorimotorické vrátkovanie u človeka
Vplyv transkraniálnej stimulácie mozgu jednosmerným prúdom na senzorimotorické vrátkovanie u človeka
Program: VEGA
Project leader: MUDr. Jagla Fedor, CSc.
Annotation: The term sensorimotor gating refers to a basic inhibitory process, which prevents processing of and reacting to irrelevant stimuli so that resources can be allocated to salient aspects of the environment. Disrupted gating is considered to play a causal role in the development of psychosis in schizophrenia spectrum disorders. Prefrontal cortex (PFC) is involved in inhibitory control processes and its dysfunction is a hallmark of schizophrenia. Transcranial direct current stimulation stimulation (tDCS) can be used to increase or decrease excitability of neuronal tissue and is increasingly used to modulate human brain activity and cognitive processes. In this project, we will systematically explore in healthy adults the possibility to modulate sensorimotor gating by tDCS of the PFC, which has not been investigated so far. Our findings will bring important new knowledge on the brain mechanisms of sensorimotor gating, pathogenesis of mental disorders and possibilities of their treatment.
Duration: 1.1.2017 – 31.12.2019
Interakcia nitrergickej, neurotrofickej a endokrinnej signalizácie v etiopatogenéze schizofrénie
Interaction of nitrergic, neurotrophic and endocrine signaling in the etiopathogenesis of schizophrenia
Program: SRDA
Project leader: MUDr. Riečanský Igor, PhD.
Annotation: Schizophrenia is a common and severe mental disorder but its pathogenesis is yet poorly understood. Susceptibility to schizophrenia is largely genetic but the genetic predisposition is determined in a complex network of interactions between multiple risk genes and environmental factors, resulting in disordered brain development and function. There is increasing evidence that chronic stress and dysregulation of several signaling pathways plays role in the neurodevelopmental impairment underlying schizophrenia. In this project, by adopting a multidisciplinary approach, we will address at several levels (genetic, neurobiological and behavioral) a candidate pathophysiological pathway, involving nitrergic, neurotrophic and stress signaling, which might be importantly involved in the disordered brain development in schizophrenia. A focus on schizophrenia endophenotypes will enable us to integrate findings from human subjects at genetic risk of the disorder with those from a rodent neurodevelopmental model of schizophrenia. This project will bring important new knowledge on the etiopathogenesis of this devastating disorder and potential novel strategies of its treatment.
Duration: 1.7.2015 – 30.6.2019
Návrh a implementácia metodiky pre rehabilitáciu pacientov s bolesťami chrbta s využitím zrakového biofeedbacku
Design and implementation of visual biofeedback for the rehabilitation of mobility deficiencies in patients with low back pain
Program: SRDA
Project leader: Ing. Hlavačka František, CSc.
Annotation: The main goal of this project is to design, optimize and implement a specialized method for the improved rehabilitation of mobility deficiencies in patients suffering from low back pain (LBP). The system will be equipped with accurate inertial measuring units embedded with highly sensitive micro-electro-mechanical (MEMS) accelerometers and gyroscopes, as well as a force platform providing input signals that will be processed and displayed back to the patient (visual biofeedback). LBP is a worldwide health problem affecting people of all ages, with recurring symptoms. Based on positive long-term experiences with visual biofeedback, the project strives to design an effective rehabilitation program for the improvement of impaired trunk mobility during sitting, and also impaired balance control during stance in LBP patients. The project not only includes the accurate acquisition of postural data, but also the development of software which will process, interpret and display this data in an easy to understand way. The software is intended to be easily operated and include training tasks that can be personalized to patient specific requirements. The ultimate goal of the project is to implement complex sensor systems capable of accurate measurements but process the data into an easy to use and easy to interpret rehabilitation tool for improving postural and motor function deficiencies in patients suffering from LBP.
Duration: 1.7.2017 – 30.6.2019
NANOSIMKA – Účinok nanoenkapsulovaného simvastatínu na kardiovaskulárny systém pri experimentálnom metabolickom syndróme
Effects of nanoencapsulated simvastatin on cardiovascular system in experimental metabolic syndrome
Program: SRDA
Project leader: doc. RNDr. Pecháňová Oľga, DrSc.
Duration: 1.7.2015 – 30.6.2019
MVTS: Podnetné organické syntézy inšpirované prírodou – od chémie prírodných produktov po objav liečiv
Program: Other projects
Project leader: RNDr. Horáková Ľubica, PhD.
Annotation:
Duration: 1.3.2015 – 31.3.2019
Efekt lyofilizátu Cornus mas L. na kardiometabolické a zápalové parametre pri experimentálnom metabolickom syndróme
Effect of lyophylisate Cornus mas L. on cardiometabolic and inflammatory parameters in experimental metabolic syndrome
Program: VEGA
Project leader: Doc.MUDr. Lietava Ján, CSc.
Duration: 1.1.2016 – 31.12.2018
Inhibícia proliferácie a indukcia apoptózy v rakovinových bunkách ovplyvnením ich metabolického profilu
Inhibition of proliferation and induction of apoptosis in cancer cells by affecting the metabolic profile
Program: VEGA
Project leader: RNDr. Blaškovič Dušan, PhD.
Annotation: The aim of the project is to inhibit the growth and induce apoptosis in cancer cells by affecting the following metabolic pathways: glycolysis, Krebs cycle, the pentose phosphate cycle and fatty acid synthesis. Cancer cells HeLa ( cervix ) , HCT – 116 ( colon ) and MCF – 7 ( breast ) will be incubated with inhibitors of particular metabolic pathways to determine the pathway, which inhibition caused the greatest decrease of the growth, respectively the induction of apoptosis . The project is also aimed on the study of the effect of inhibiting of various metabolic pathways by natural substances, which are non-toxic for the normal cells. Multiple pathways will be simultaneously inhibited in cancer cells using natural substances to reach the most significant suppression of cancer cell growth and/or induction of apoptosis. Research will also focus on determation of the impact of coltsfoot extract on selected metabolic pathways and viability of cancer cells.
Duration: 1.1.2015 – 31.12.2018
Mechanizmy, skorá detekcia a terapia asfyktického poškodenia v perinatálnom období – porovnanie experimentálnych údajov s klinickým obrazom asfyktického novorodenca
Mechanisms, early detection and therapy of asphyxial injury in perinatal period – comparison of experimental data with clinical observation of asphyxial newborns
Program: VEGA
Project leader: Doc. RNDr. Ujházy Eduard, CSc.
Annotation: The results from our studies concerning uncovering the mechanisms and possibilities of early detection of embryo-fetal damage confirmed that our proposed model of subchronic perinatal asphyxia using rat model is suitable for next studies. We will use this model for further expansion of knowledge about the mechanisms of actions of asphyxia in the process of late organogenesis and perinatal period. It is important to detect suitable biochemical and morphological markers to reduce the risk of complications due to asphyxia during development, as well as the possibility of its early therapy via antioxidant active ingredients of natural and synthetic origin. The ability of these compounds to bind to transport blood components (albumin, hemoglobin) will be monitored and a method for testing the effects on neuronal cell cultures will be developed. Integral part of this project will be correlations of given data from experimental studies with clinical observations in full-term asphyxiated newborns.
Duration: 1.1.2015 – 31.12.2018
Názov projektu: Výskum ovplyvnenia zápalu, chronickej autoimunitnej reakcie a redoxnej regulácie organizmu v experimentálnej artritíde použitím nových látok pre adjuvantnú terapiu reumatoidnej artritídy
Program: VEGA
Project leader: PharmDr. Bauerová Katarína, PhD., DrSc.
Duration: 1.1.2015 – 31.12.2018
Ochrana srdca pred maladaptívnou remodeláciou extracelularnej matrix a skúmanie mechanizmov jej regresie.
Protection of the heart from maladaptive extracellular matrix remodeling and searching the mechanisms of its regression.
Program: VEGA
Project leader: RNDr. Szeiffová Bačová Barbara, PhD.
Annotation: Heart diseases are accompanied by exracellular matrix remodeling and fibrosis resulting in development of heartfailure and malignant arrhythmias. Fibrosis is a major medical problem without existing cure. However, there arecardioprotective compounds that exhibit antifibrotic effects but underlying mechanisms are poorly understood.This project is aimed to characterize key factors implicated in profibrotic signaling in rats suffering fromhypertension, diabetes, hypothyroidism and post-infarction injury and to determine targets of examinedpharmacological and nonpharmacological compounds. This approach should reveal signaling pathways andfactors, whose modulation could hamper or reverse fibrosis. It is expected that findings of this preclinical studymay outline design of clinical trials how to protect the heart from its dysfunction by non-invasive way.
Duration: 1.1.2015 – 31.12.2018
Protekcia hypertenzného a zlyhávajúceho srdca blokátorom I(f) kanálu ivabradínom: porovnanie s ACE-inhibíciou a melatonímom
Protection of hypertensive and failure heart by I(f) channel blocker ivabradin: comparison with ACE inhibition and melatonin
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga, DrSc.
Duration: 1.1.2015 – 31.12.2018
Protektívne účinky prírodných a syntetických látok pred oxidačným poškodením vysokomolekulového hyalurónanu, izolovaných živočíšnych buniek a ich mitochondrií
Protective effects of natural and synthetic substances against oxidative damage of high-molar-mass hyaluronan, isolated mammal cells and their mitochondria
Program: VEGA
Project leader: RNDr. Valachová Katarína , PhD.
Annotation: Hyaluronan (HA) is a polysaccharide of molar mass several megaDaltons present in tissues of vertebrates. In inflammatory diseases the average molar mass of HA decreases by action of oxidants. A significant decrease of synovial fluid (SF) viscoelasticity was detected, thereby its lubricating properties are worsened. High-molar-mass HA solutions are relevantly used for in vitro studies of free radicals and oxidants performance and also for a study of protective effects of antioxidants/drugs in a role of prevention or chain-breaking degradation of HA macromolecules.Substances of synthetic origin such as antiinflammatory drugs, mitochondria targeted antioxidants and natural substances with demonstrated antioxidative effects against HA degradation will be tested as protectors of oxidative degradation of cell lines of fibroblasts NIH-3T3, B-HNF-1, VH10 and cell organels, especially mitochondria.
Duration: 1.1.2015 – 31.12.2018
Protektívny účinok NO a CO donorov pri experimentálnom infarkte myokardu s hypertenzívnymi komplikáciami
Protective effect of NO and CO donors in experimental myocardial infarction with hypertensive complications
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga, DrSc.
Duration: 1.1.2015 – 31.12.2018
Redoxná regulácia profesionálnych fagocytov v krvi a v centrálnom nervovom systéme: Molekulárne mechanizmy a funkčný význam.
Redox control of the professional phagocytes in blood and in the central nervous system: Molecular mechanisms and functional significance.
Program: VEGA
Project leader: PharmDr. Jančinová Viera, PhD.
Annotation: The submitted project is a continuation of our previous research in the pharmacological regulation of inflammatory processes. The focus is on neutrophils and microglial cells (resident brain macrophages) which are considered active participants in the initiation and progression of pathological states connected with chronic inflammation such as arthritis or neurodegenerative diseases. The aim of the project is to explain cellular and molecularmechanisms involved in the pharmacological modulation of these cells. Attention will be paid to drugs and derivatives of natural substances which are able to influence the production of reactive oxygen and nitrogen species. The coordinated research of two types of phagocytes creates an opportunity for the sharing of modern instruments and excellent methods as immunofluorescence microscopy or flow cytometry. Cooperation betweenbasic research and clinical practice will allow the use of these methods in the analysis of phagocytes from patients with rheumatoid arthritis.
Duration: 1.1.2016 – 31.12.2018
Rizikové faktory kardiovaskulárnych a cerebrovaskulárnych ochorení a farmakologické možnosti ich ovplyvnenia
Risk factors of cardiovascular and cerebrovascular diseases and pharmacological possibilities of their influence
Program: VEGA
Project leader: RNDr. Gáspárová Zdenka, PhD.
Annotation: The project is aimed at the study of mechanisms of etiopathogenesis of metabolic syndrome (MS) and metabolic cognitive syndrome (MSC). The goal is to design new effective therapy affecting the origin and development of risk factors of cardiovascular and cerebrovascular diseases. Experiments will be performed on a genetic model of rats with hereditary hypertriglyceridemia, fed with hyperlipidemic diet. The induced metabolic disorders willbecome manifest by hypertension, hypercholesterolemia, hyperglycemia, hypertriglyceridemia, inflammatory reactions and increased markers of oxidative stress. Effect on the risk factors of MS and MCS, lipidic profile, on markers of damage at cellular and molecular level, function, behavioral and histopathological changes will be studied after repeated administration of the new pyridoindole SMe1EC2, flavonoid rutin, and atorvastatin as a standard. Action on several risk factors of cardiovascular and cerebrovascular diseases simultaneously is expected.
Duration: 1.1.2015 – 31.12.2018
Štúdium dôsledkov materskej depresie a podávania antidepresíva venlafaxínu na funkčný vývin mozgu a správanie potomstva potkanov
Study of consequences of maternal depression and antidepressant venlafaxine treatment on functional development of the brain and behavior of rat offspring
Program: VEGA
Project leader: RNDr. Dubovický Michal, CSc.
Duration: 1.1.2015 – 31.12.2018
Vplyv endogénnej hladiny oxidu dusnatého a sírovodíka na tlak krvi, pulzovú vlnu, funkciu a štruktúru cievnej steny
Program: VEGA
Project leader: RNDr. Kristek František, DrSc.
Duration: 1.1.2017 – 31.12.2018
Vplyv konštitučných faktorov redoxnej regulácie na endofenotypové znaky schizofrénie
The influence of constitutional factors of redox regulation on endophenotypic markers of schizophrenia
Program: VEGA
Project leader: MUDr. Riečanský Igor, PhD.
Annotation:
Duration: 1.1.2016 – 31.12.2018
Vplyv veku na senzorickú reguláciu rovnováhy pri vstávaní zo sedu a chôdzi
Age-related changes in sensory control of balance during sit-to-stand and gait
Program: VEGA
Project leader: Ing. Hlavačka František, CSc.
Annotation: The ability to reliably perform functional movements like sit-to-stand, gait or maintaining the erect posture is a basis for the independent living. Difficulties with conducting these motor activities increase with age. Early diagnostics and suitable therapeutic interventions can help to moderate these problems and prevent falls. The goal of our project is to obtain new knowledge about the physiological mechanisms of sit-to-stand movement and gait in young and elderly adults, and also to expand information about the sensory influence on these motor functions. We will focus on analysis of balance control in static and dynamic conditions during sit-to-stand, step initiation and gait using unique 6-camera motion capture system BTS SMART-DX. We assume that results of our project would be worthwhile in designing of perspective rehabilitative methods for elderly people with mobility deficit to improve their balance and functional mobility, and also to enhance diagnostics of early stages of neurological disorders.
Duration: 1.1.2016 – 31.12.2018
KANASTA – Kardiovaskulárne účinky nanoenkapsulovaného simvastatínu a koenzýmu Q10 pri experimentálnej hyperlipidémii (KANASTA)
Cardiovascular Effects of Nanoencapsulated Simvastain and Coenzyme Q10 in Experimental Hyperlipidemia (KANASTA)
Program: Other projects
Project leader: RNDr. Reháková Radoslava
Annotation: The project is aimed to investigate cardiovascular effects of simvastatin together with CoQ10 in experimental hyperlipidemia and to increase bioavailability of simvastatin in the liver, thus reducing the daily dose and consequently to prevent the reduction of CoQ10 levels.
Duration: 27.11.2015 – 26.11.2018
Výskum inovatívnych liekových foriem a technologických postupov pri ekologickom spracovaní biologického odpadu vaječných škrupín
Program: Vedecko-technické projekty
Project leader: RNDr. Dubovický Michal, CSc.
Duration: 1.9.2015 – 31.8.2018
Možná duálna funkcia P-glykoproteínu pri viacliekovej rezistencii leukemických buniek: efluxná pumpa a regulačný proteín.
Program: SRDA
Project leader: RNDr. Barančík Miroslav, DrSc.
Duration: 1.7.2014 – 30.6.2018
Aldoketoreduktázy v chronických ochoreniach – in silico modelovanie významných enzýmov a ich komplexov s indolovými derivátmi
Aldo-keto reductases in chronic diseases – in silico modeling of significant enzymes and their complexes with indole derivatives
Program: VEGA
Project leader: RNDr. Májeková Magdaléna, PhD.
Annotation: The aldo-keto reductases belong into a family of enzymes characterized as monomeric NADPH-dependentoxidoreductases. Till now the number of more than 100 enzymes from this family is known. They are divided according to their substrate specificity to individual subgroups. Apart from their beneficial physiological functions hold by biosynthesis, metabolism and detoxication, they can play also a negative role by various pathologic processes as chronic diabetic complications, inflammation, carcinogenesis, asthma etc. The aim of this work is toelaborate and test the specific models of significant subgroups of aldoketoreductases by means of in silico methods. The individual models are to be used for considering the inhibition properties of indole compounds with measured or perspective biological activity.
Duration: 1.1.2014 – 31.12.2017
Bioenergetické aspekty ochrany myokardu pomocou remote ischemického preconditioningu. Úloha srdcových mitochondrií
Bioenergetic aspects of myocardial protection by means of remote ischemic preconditioning. The role of cardiac mitochondria
Program: VEGA
Project leader: Ing. Ferko Miroslav, PhD.
Annotation: Any sufficiently strong challenge induced by physiological or pathological stimuli leads to myocardial changes that can be considered as abnormal. These changes partially reflect endogenous protective processes. Pathological stimuli triggering the endogenous protection are hypoxia, ischemia, diabetes mellitus and hypertension. Cardioprotective approaches involve pharmacological and ischemic preconditioning (IP) as well as clinically applicable form of IP, so called remote ischemic preconditioning (RIP). Numerous mechanisms of RIP induction and effects have been investigated. Still, the character of the signals from the site of RIP leading to target structures in the heart is not elucidated. Little is known also with respect to bioenergetics aspects and role of cardiac mitochondria in RIP induced cardioprotection. The aim of this project is to contribute to elucidation of the molecular mechanisms of RIP and role of mitochondria in the signaling processes of RIP and heart adaptation to hypoxia and ischemia.
Duration: 1.1.2015 – 31.12.2017
Deriváty kyseliny 1-indoloctovej ako inhibítory aldózareduktázy: dizajn, syntéza a biologická aktivita
Indole-1-acetic acid derivatives as aldose reductase inhibitors: design, synthesis and biological activity
Program: VEGA
Project leader: Ing. Štefek Milan, CSc.
Duration: 1.1.2015 – 31.12.2017
Matrix metaloproteinázy, microRNAs a deformabilita erytrocytov – nové diagnostické a prognostické biomarkery srdcového zlyhávania
Matrix-metalloproteinases, microRNAs and deformability of erythrocytes as a novel diagnostic and predictive biomarkers of heart failure
Program: VEGA
Project leader: doc. RNDr. Barteková Monika, PhD.
Annotation:
Duration: 1.1.2014 – 31.12.2017
Molekulárne mechanizmy zahrnuté v účinkoch doxorubicínu u zvierat s rozvinutou hypertenziou a možnosti ovplyvnenia účinkov doxorubicínu pôsobením kvercetínu.
Molecular mechanisms involved in the effects of doxorubicin in rats with developed hypertension and ways of modulation of of these effects of doxorubicin by quercetin.
Program: VEGA
Project leader: RNDr. Barančík Miroslav, DrSc.
Duration: 1.1.2015 – 31.12.2017
Nové molekulárne mechanizmy poškodenia kardiovaskulárneho systému ionizujúcim žiarením a možnosti jeho cielenej medikamentóznej prevencie.
Program: VEGA
Project leader: D.h.c., Prof., MUDr. Slezák Ján, DrSc., FIACS
Duration: 1.1.2015 – 31.12.2017
Oxid dusnatý a redoxný stav mozgu v experimentálnom neurovývinovom modeli schizofrénie
Nitric oxide and brain redox status in an experimental neurodevelopmental model of schizophrenia
Program: VEGA
Project leader: RNDr. Vranková Stanislava, PhD.
Annotation: Schizophrenia is a severe mental disorder. Research suggests that an important role its pathophysiology may play the oxidative stress, i.e., redox imbalance in neurons of the central nervous system, leading to an impairment of brain development. This redox imbalance might be caused by an overproduction of nitric oxide (NO). Therefore, this project is focused at the role of NO in brain development. We will adopt the postweaning social isolation rearing of rats, which is an established neurodevelopmental model of schizophrenia. We will explore whether social isolation affects NO synthesis in the brain and whether NO production is linked with redox status of the brain tissue and schizophrenia-like behavioral alterations. We will also explore whether the changes in neurobiology and behavior, induced by isolation rearing, can be positively influenced by an antioxidative treatment. Results of this project will contribute to elucidating the pathogenesis of schizophrenia and its treatment possibilities.
Duration: 1.1.2015 – 31.12.2017
Signálne dráhy NO a H2S a ich interakcia v regulácii cievneho tonusu počas skorej fázy vývoja experimentálnej hypertenzie
NO and H2S signal pathways and their interaction in the control of vascular tone during early developmental stage of experimental hypertension
Program: VEGA
Project leader: RNDr. Čačányiová Soňa, PhD.
Annotation: Nitric oxide (NO) and hydrogen sulphide (H2S) belong to important gaseous molecules engaged to vascular smooth muscle regulation during normotensive as well as hypertensive conditions. In adult rats it is well documented the role of NO originated from endothelial isoform of NO-synthase, the specificity and originality of NO released from neuronal isoform of enzyme as well as the existence of specific NO-H2S interaction – not just yet identified nitroso-sulphide signalization. The aim of the project is to characterize and compare the role of NO and H2S, their signal pathways and interaction, in young normotensive rats and in rats with developing experimental hypertension(NO-deficient and essential hypertension. Simultaneous following of both signal pathways on the same vessels isolated from normotensive and hypertensive rats and humans represents the actual way how to associate the results of basic research with clinical praxis.
Duration: 1.1.2014 – 31.12.2017
Štúdium klinicky využiteľných foriem preconditioningu ako alternatívnej metódy ochrany myokardu pred akútnou ischémiou v organizme zaťaženom civilizačnými ochoreniami
Study of the clinically relevant forms of preconditioning as an alternative method of myocardial protection against acute ischemia in the organism challenged with civilization diseases
Program: VEGA
Project leader: MUDr. Ravingerová Táňa, DrSc., FIACS
Annotation: Hypertension, metabolic disorders, sex and aging have a negative impact on the adaptive mechanisms of innate cardioprotection and alter heart survival upon acute ischemia/reperfusion (I/R). These comorbidities suppress the mechanisms of cell signaling involved in protective effects of ischemic preconditioning (PC) and accelerate necrotic and apoptotic processes during I/R, reduce energy production and contribute to enhanced arrhythmogenesis. The project is aimed to investigate the possiblities to restore adaptive potential in the pathologically altered myocardium including aged heart by means of modulation of the intensity and forms of adaptive stimuli. Effects of clinically relevant forms of PC: „remote“ PC of the heart induced by ischemia of the distant organ and its pharmacological simulations by drugs regulating energy metabolisms and involved in the mechanisms of PC, as well as their genomic and non-genomic effects including the impact on mitochondrial function and apoptotic cascade will be investigated
Duration: 1.1.2015 – 31.12.2017
Úloha perivaskulárneho tukového tkaniva v regulácii cievneho tonusu u potkanov s kardiovaskulárnou dysfunkciou
The role of perivascular adipose tissue in the regulation of vascular tone in rats with cardiovascular dysfunction
Program: VEGA
Project leader: Mgr. Zemančíková Anna, PhD.
Annotation: Perivascular adipose tissue (PVAT) which surrounds most of the systemic arteries is considered to be an important modulator of their functions. Relaxant factors produced by this tissue inhibit vascular contractile responses to many vasoconstrictor substances. The aim of this project is to analyse the mechanisms which are responsible for this modulatory effect. In addition to the direct action of the PVAT-released substances on vascular smooth muscle we intend to study also the possible interaction of PVAT with vascular sympathetic nervous system. These interactions are important for maintaining vascular tone in physiological as well as in pathological conditions associated with vascular dysfunction, e.g. in hypertension. Our goal is to discover whether the changes in paracrine functions of PVAT may participate in the origin of vascular disorders and whether they precede the abnormalities of vascular reactivity at the level of smooth muscle.
Duration: 1.1.2015 – 31.12.2017
Vzťah kognitívnych schopností a funkčnej asymetrie mozgových heimsfér
Cognitive skills form the perspective of functional asymmetry of the brain
Program: VEGA
Project leader: RNDr. Cimrová Barbora, PhD.
Annotation: Recent research using dichotic stimulation and a visual half-field technique indicate that sex differences in cognitive skills could be connected with lateralization of perceptual cognitive functions particularly because men and women differ not only in cognitive skills but also in degree of lateralization of cognitive functions. In general, perceptual asymmetries are greater in men. An assumtion is that a certain degree of the leftward lateralization (but not the maximal lateralization) is optimal for verbal functions, so that high left hemisphere lateralization is not associated with superior performance. Therefore, women outperform men in verbal tasks. In nonverbal task, a greater degree of lateralization towards the right hemisphere is associated with better performance. The proposed project will try to answer a question whether a degree of lateralization could explain the variation in performance within the groups of men and women. The sex differences in lateralization will be explored as well.
Duration: 1.1.2015 – 31.12.2017
Chemoenzymatická syntéza a hodnotenie biologických aktivít prírodných glykofenolík a ich analógov.
Chemoenzymatic synthesis and evaluation of biological activities of natural glycophenols and their analogues
Program: SRDA
Project leader: RNDr. Barančík Miroslav, DrSc.
Duration: 1.10.2013 – 30.9.2017
Štúdium regulácie radikálovej a bunkovej signalizácie v hypertenzii a vplyv nových terapií na túto signalizáciu.
Study of regulation of radical and cellular signaling during hypertension and influence of novel therapies on this signaling
Program: SRDA
Project leader: RNDr. Barančík Miroslav, DrSc.
Duration: 1.10.2013 – 30.9.2017
HYPERAD – Štúdium regulácie radikálovej a bunkovej signalizácie v hypertenzii a vplyv nových terapií na túto signalizáciu.
Study of regulation of radical and cellular signaling during hypertension and influence of novel therapies on this signaling.
Program: SRDA
Project leader: doc. RNDr. Dovinová Ima, PhD.
Annotation: New project was started in october 2013. This year we studied mechnisms involved in blood pressure regulation of young SHR after treatment of PPAR gamma agonist- pioglidazone (PIO). Application of PIO significantly influenced development of high blood pressure and improved lipid profile and vessels relaxation. PIO ifluenced also: i) regulation of SOD enzymes, ii) renin-angiotenzin system a iii) intracellular signaling pathways of Akt kinase and beta-catenin. Outputs: 1 publication CC.
Duration: 1.10.2013 – 30.9.2017
Ako synchronizácia formuje sociálne väzby: mechanizmy a neuronálne dráhy
How synchrony shapes human social bonding: mechanisms and neural pathways
Program: SASPRO
Project leader: MUDr. Riečanský Igor, PhD.
Annotation: Human societies are characterized by flexibly changing social groups with remarkably strong bonds. A striking, universal characteristic of bonded groups is a widespread tendency their members to synchronize their movements. Such behavioral synchronization supports deindividuation, cooperation, and group solidarity. However, the psychological and neural mechanisms of the link between synchrony and bonding are poorly understood. This project aims to fill this gap by using a multi-level social neuroscience approach based on a series of behavioral, functional neuroimaging and psychopharmacological experiments. Insights from the project will advance our understanding of the complex processes of social behavior. The principal investigator of this project is Dr. Jasminka Majdandzic.
Duration: 1.5.2015 – 20.9.2017