Current Projects

International

LOGIC LAB – Molecular logic lab-on-a-vesicle for intracellular diagnostics
Molecular logic lab-on-a-vesicle for intracellular diagnostics
Program: Horizon 2020
Project leader: RNDr. Mach Mojmír PhD.
Annotation: A dysfunction of cells lining the inner walls of blood vessels, i.e. the endothelium, is the primary cause of many lifestyle related diseases. According to the WHO, those diseases accounted for 60% of all deaths worldwide in 2005. Tailor-made diagnostic tools for early and reliable identification of endothelial dysfunction are urgently needed both in fundamental research and clinical routine, respectively.The Marie Skłodowska-Curie action LOGIC LAB objects to develop and characterize innovative molecular logic gates that can be applied as advanced diagnostic tools for parallel analyte sensing in live mammalian cells. Thereby, providing a unique method to discover endothelial dysfunction and the onset of diseases much easier and earlier than so far.LOGIC LAB creates a multi-faceted and multi-sectoral research environment for the next generation of scientists in order to establish a novel type of molecular logic sensors that reliably operate in biological media – a crucial requirement for their application i.e. as rapid and easy-to-handle tools for intracellular diagnostics.With excellent cross-disciplinary scientific and complementary training provided in the network, we aim to educate highly-skilled young scientists in the fields of chemistry, physics and biology, who will significantly strengthen the international research community in the domain of molecular logic sensing. Thus, in the long term, LOGIC LAB aims to finally bridge the gap between lab bench and biological or medical practice. It is this gap, that so far prevents a wide-ranging use of existing molecular logic gates e.g. for the diagnosis of lifestyle-associated diseases.
Duration: 1.11.2018 – 31.10.2022
CardioRNA – Katalýza transkriptomického výskumu kardiovaskulárnych ochorení
Catalysing transcriptomic research in cardiovascular disease
Program: COST
Project leader: doc. RNDr. Barteková Monika PhD.
Duration: 3.10.2018 – 2.10.2022
EU-CARDIOPROTECT – Realizácia terapeutického potenciálu nových kardioprotektívnych terapií
Realising the therapeutic potential of novel cardioprotective therapies
Program: COST
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: The proposed COST Action will set up a pan-European Research Network of leading experts in cardioprotection, to jointly develop new initiatives and new strategies for finding innovative and more effective approaches to cardioprotection and for optimizing the pre-clinical and clinical evaluation of new cardioprotective therapies, so as to improve their translation into the clinical setting for patient benefit. The COST Action will co-ordinate and strengthen European research in the field of cardioprotection and accelerate scientific progress through the dissemination and sharing of new therapeutic targets, among network members and industrial partners, thereby facilitating the discovery of new cardioprotective therapies. By utilizing the joint expertise of different European network members we will investigate factors which confound the efficacy of new cardioprotection therapies including comorbidities (such as age, diabetes, and hypertension) and co-medications (such as anti-platelet therapies, statins and beta-blockers). Finally, we will set up a European network of research centers for multi-center laboratory testing of new cardioprotective therapies using small and large animal models of acute IRI in order to select those therapies most likely to succeed in the clinical setting. All aspects of this COST Action proposal require a critical mass of partners across a wide geographic distribution across Europe in order to deliver the objectives outlined in this proposal. The discovery of novel signaling pathways and targets underlying cardioprotection both within and outside the cardiomyocyte (WG1 NEW TARGETS), and the testing of different combinations of cardioprotective therapy (WG2 COMBINATION THERAPY) requires investigators with different experience and expertise across Europe. The ability to test the effect of confounders of cardioprotection (WG3 CONFOUNDERS) requires the expertise of different partners in the different co-morbidities and testing of co-medications. Finally, the most important objective of this COST Action proposal, requires the setting up of a Europe-wide research network for (a) multicenter testing of novel cardioprotective therapies using small and large animal models (WG4 CONSORTIUM) and (b) testing of novel cardioprotective therapies in proof-of-concept clinical studies and optimization of multi-center clinical outcome cardioprotection studies. By definition this requires a critical mass of research partners distributed across Europe.
Duration: 19.10.2017 – 18.10.2021
Anti-inflammatory effect of astaxanthin, sulforaphane and Crocus sativus extract evaluated in two rodent models of age related diseases.
Program: Inter-academic agreement
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Duration: 1.1.2018 – 31.12.2020
Skúmanie mechanizmov antiarytmických účinkov melatonínu.
Investigation of the mechanims involved in antiarrhythmic effects of melatonin
Program: Bilateral – other
Project leader: RNDr. Tribulová Narcisa DrSc.
Duration: 1.5.2014 – 31.12.2020
SKPTPHARMACOL – Spolupráca na komplexnom hodnotení farmakologického ovplyvnenia zápalových ochorení pohybového aparátu a gastrointestinálneho traktu na experimentálnych zvieracích modeloch
Collaboration on a complex pharmacological assessment of inflammatory diseases of the musculo-skeletal system and gastrointestinal tract on experimental animal models
Program: Bilateral – other
Project leader: PharmDr. Bauerová Katarína PhD., DrSc.
Duration: 1.1.2019 – 31.12.2020
Śtúdium mechanizmov spúšťania a prenosu kardioprotektívných signálov indukovaných neinvazívnymi adaptačnými stimulmi
Study of the triggering mechanisms and transmission of cardioprotective signals induced by noninvasive adaptive stimuli
Program: Inter-academic agreement
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Cardiovascular diseases are one of the leading causes of mortality in modern society and predicted to rise over the coming decades, due to aging population, longer survival after myocardial infarction, and incidence of civilization diseases. Research pointed out to the protective effects of phenomenon termed ischemic preconditioning (IPC), especially its novel clinically acceptable and safer forms. Currently, cellular mechanisms activated by stimuli like exercise, hypoxia and PC of the remote organ are not yet completely clear as compared with the classiccal IPC. For that reason, pathological animal models (myocardial ischemia, hypertension, d. mellitus, dyslipidemia) and aged animals will be used. Acute PC-like settings of above mentioned interventions, and their longer lasting adaptation modalities will be tested in the in vivo and the ex vivo rats using relevant methodology (combination of physiological, pharmacological and biochemical techniques). The results obtained in this project may lead to development of novel or modified therapeutic strategies to manage myocardial ischemia in patients.
Duration: 1.1.2018 – 31.12.2020
Study the role of iron oxide nanoparticles in a model of hypertension and comorbid Alzheimer\’s disease
Study the role of iron oxide nanoparticles in a model of hypertension and comorbid Alzheimer\’s disease
Program: Inter-academic agreement
Project leader: RNDr. Bernátová Iveta DrSc.
Duration: 1.1.2018 – 31.12.2020

National

Program: SRDA
Project leader: RNDr. Gáspárová Zdenka PhD.
Duration:
Vazoaktívne účinky sulfidovej signalizácie a jej interakcia s oxidom dusnatým v rôznych animálnych modeloch metabolického syndrómu
Program: SRDA
Project leader: Ambrosová Ľubica
Duration: 1.1.2019 –
Vazoaktívne účinky sulfidovej signalizácie a jej interakcia s oxidom dusnatým v rôznych animálnych modeloch metabolického syndrómu
Program: VEGA
Project leader: Mgr. Berényiová Andrea PhD.
Annotation: Nitric oxide (NO) and hydrogen sulphide (H2S) are signalling molecules involved into the regulation of the arterial tone. The synthesis of both has been shown in arterial wall, moreover their contribution in physiological (relaxation of arterial smooth muscle cells) and pathophysiological processes (hypertension, diabetes mellitus, atherosclerosis) have been already proved. Metabolic syndrome (MS) is a group of abnormalities including obesity, hypertension, hyperlipidemia which that together increase the risk of developing cardiovascular disease. Studies have characterised H2S signalisation and NO-H2S interaction especially in normotensive rats, information about their role in experimental hypertension are just limited and about their engagement into the etiopathogenesis of metabolic syndrome is totally missing. The main goal of this project is to describe the vasomotoric role of NO and H2S in different models of MS: induced by high-fructose diet, by high-fat diet in normotension and primary hypertension.
Duration: 1.1.2019 –
Prenatálne programovanie chorôb v dospelosti: možnosti terapie a prevencie následkov prenatálnej hypoxie u potomstva potkanov
Prenatal programming of adult diseases: treatment and prevention of outcomes of gestational hypoxia in rat offspring
Program: VEGA
Project leader: RNDr. Mach Mojmír PhD.
Annotation: Hypoxia during pregnancy, labor or early life stage is a major determinant of neurological morbidity and mortality in the neonatal period. In the last decade the fetal origin of chronic adult diseases was proposed as the most important factor in genesis of diabetes and hypertension in adulthood. The scientists showed that malnutrition, and inadequate oxygen supply during embryofetal development may lead to the inadequate apoptosis/necrosis, and caused maldevelopment of the organs responsible for regulation blood pressure, glucose, or improper brain wiring. Although the understanding of perinatal asphyxia-related pathophysiology is gradually increasing, limited therapeutic options are available to prevent or even mitigate the devastating process that unfolds after injury. Mitochondria-targeted antioxidants (MTA) are one of the most important therapies for providing neuroprotection in cerebral ischemia. The aim of the project will be to explore the possibilities of using MTA in late gestational hypoxia model.
Duration: 1.1.2020 – 31.12.2023
Prenatálne programovanie chorôb v dospelosti: možnosti terapie a prevencie následkov prenatálnej hypoxie u potomstva potkanov
Prenatal programming of adult diseases: treatment and prevention of outcomes of gestational hypoxia in rat offspring
Program: VEGA
Project leader: RNDr. Mach Mojmír PhD.
Annotation: Hypoxia during pregnancy, labor or early life stage is a major determinant of neurological morbidity and mortality in the neonatal period. In the last decade the fetal origin of chronic adult diseases was proposed as the most important factor in genesis of diabetes and hypertension in adulthood. The scientists showed that malnutrition, and inadequate oxygen supply during embryofetal development may lead to the inadequate apoptosis/necrosis, and caused maldevelopment of the organs responsible for regulation blood pressure, glucose, or improper brain wiring. Although the understanding of perinatal asphyxia-related pathophysiology is gradually increasing, limited therapeutic options are available to prevent or even mitigate the devastating process that unfolds after injury. Mitochondria-targeted antioxidants (MTA) are one of the most important therapies for providing neuroprotection in cerebral ischemia. The aim of the project will be to explore the possibilities of using MTA in late gestational hypoxia model.
Duration: 1.1.2020 – 31.12.2023
Experimentálna liečba neonatálnej hypoxicko-ischemickej encefalopatie (nHIE): potenciácia hypotermickej neuroprotekcie melatonínom u novorodených potkanov
Experimental therapy of neonatal hypoxic-ischemic encephalopathy (nHIE): potentiation of hypothermic neuroprotection by melatonin in newborn rats
Program: VEGA
Project leader: RNDr. Juránek Ivo PhD., DrSc.
Annotation: Neonatal hypoxic-ischemic encephalopathy (nHIE) is among most serious causes of mortality and morbidity in newborns. Efficacy of current nHIE treatment is rather low. Routinely used therapeutic hypothermia (HT) is only partially effective. To augment hypothermic neuroprotection, drugs like erythropoietin, anticonvulsants, antioxidants and inert gases are tested. In this project, using newborn rats, we will study possible augmentation of hypothermia effect by combining HT with melatonin (MEL)-derived antioxidants. We will assess brain damage and efficacy of each intervention by various techniques, including noninvasive in vivo MRI and MRS, histology and neurobehavioral testing. Novelty of the project lies in testing our idea that MEL-derivative possessing antioxidative properties 100 fold higher than MEL will be more effective in potentiating the hypothermic effect than MEL itself. Anticipated results may help understand better nHIE mechanisms and to propose new strategies to treat birth asphyxia effectively.
Duration: 1.1.2020 – 31.12.2023
Hodnotenie biologickej kompatibility zdravotníckych pomôcok (ZP) a innovativnych materiálov pre výrobu ZP s využitím in vitro metód založených na 3D rekonštruovaných modeloch ľudského tkaniva.
Bio-compatibility assessment of medical devices and novel medical device materials using in vitro methods based on 3D reconstructed human tissue models.
Program: VEGA
Project leader: Dr.rer.nat., Ing. Kanďárová Helena ERT
Duration: 1.1.2020 – 31.12.2023
Podieľajú sa konexinové kanály v preťaženom srdcovom svale na extracelulárnej remodelácii?
Are connexin channels involved in extracellular matrix remodeling of overloaded heart?
Program: VEGA
Project leader: RNDr. Tribulová Narcisa DrSc.
Annotation: Cardiac connexin (Cx) channels that are localized at the gap junctions in intercalated discs ensure electrical and molecular signals propagation among cardiomyocytes. Such direct intercellular signaling is essential for synchronized heart contraction. Cardiovascular diseases in humans as well as in animal models are accompanied by abnormal Cx43 expression and its enhanced localization to the lateral sides of the cardiomyocytes. Consequently, it deteriorates synchronized heart function and increase a risk for malignant arrhythmias. Based on general knowledge and our studies we hypothesize that laterally localized Cx43 channels might transmit signals from cardiomyocytes into extracellular space and by this way contribute to adverse extracellular matrix remodeling. Intention of the project is to reveal the possible implication of Cx43 channels in modulation of extracellular space in diseased heart. It may stimulate to search novel approaches in protection from cardiac dysfunction and arrhythmias.
Duration: 1.1.2020 – 31.12.2023
SVBENMKVS – Skúmanie vplyvu bakteriálneho endotoxínu na mechanosenzorický komplex v srdci.
Investigation of endotoxin effects on mechanosensoric complex in the heart of normotensive rats.
Program: VEGA
Project leader: RNDr. Okruhlicová Ľudmila CSc.
Annotation:
Duration: 1.12.2020 – 31.12.2023
Štúdium nových mechanizmov kardioprotekcie voči ischemicko-reperfúznemu poškodeniu srdca: úloha extracelulárnych vezikúl, nekódujúcich RNA a vplyv metabolických komorbidít na tieto mechanizmy
Study of new mechanisms of cardioprotection against ischemia-reperfusion injury of the heart: role of extracellular vesicles, non-coding RNAs and impact of metabolic co-morbidities on these mechanisms
Program: VEGA
Project leader: doc. RNDr. Barteková Monika PhD.
Annotation: Ischemic heart disease and myocardial infarction represent major diseases associated with ischemia-reperfusion(I/R) injury of the heart. Despite several powerful cardioprotective interventions against I/R injury includingendogenous (e.g. ischemic conditioning) as well as exogenous ones including treatment with natural antioxidants,have been proposed, molecular mechanisms of cardioprotection are not fully clarified so far; moreover, there areserious translational gaps in transferring cardioprotective interventions into clinics due to comorbidities present inreal patients suffering from cardiac I/R injury. The aim of the present project is to uncover the role of extracellularvesicles as new players in cardioprotection, to identify particular non-coding RNAs involved in cardioprotection,and to explore the effect of metabolic co-morbidities on molecular mechanisms and efficiency of cardioprotection,altogether in the sake of effective transfer of experimental knowledge to human personalized medicine.
Duration: 1.1.2020 – 31.12.2023
TOXINOVAGE – Inovatívne prístupy v toxikológii starnutia
Innovative approaches in toxicology of ageing
Program: SRDA
Project leader: Ing. Račková Lucia PhD.
Duration: 1.7.2019 – 30.6.2023
Úloha matrixových metaloproteináz v patofyziológii ochorení kardiovaskulárneho systému a ich vzťah k bunkovej redoxnej signalizácii.
The role of matrix metalloproteinases in pathophysiology of cardiovascular system diseases and their relation to cellular redox signaling.
Program: SRDA
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 1.7.2019 – 30.6.2023
Experimentálna štúdia pôsobenia materskej depresie a antidepresívnej liečby počas gravidity a laktácie na zdravie matky a vývin potomstva.
Program: VEGA
Project leader: RNDr. Dubovický Michal CSc.
Annotation:
Duration: 1.1.2019 – 31.12.2022
Experimentálny infarkt myokardu: príspevok hypertenzie a obezity, účinok inhibítora toll-like receptorov.
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2019 – 31.12.2022
Modulácia dysregulácie extracelulárnej matrix a medzibunkovej komunikácie ako protekcia srdcového svalu pred jeho funkčným zlyhaním
Modulation of dysregulation of extracellular matrix and intercellular communication as a heart protection from its functional failure
Program: VEGA
Project leader: RNDr. Szeiffová Bačová Barbara PhD.
Annotation: Cardiovascular diseases are accompanied by extracellular matrix remodeling and fibrosis associated with impaired myocardial gap junction communication, what subsequently results in the development of heart failure and malignant arrhythmias. Cardiac fibrosis is one of the major problems in medicine with no effective treatment. We aimed in this project to characterize key factors involved in profibrotic signaling in rats with hypertension, altered thyroid status, post-infarction injury and to examine the possibilities of pharmacological and non-pharmacological modulation of these profibrotic factors. This approach should reveal key signaling pathways and proteins whose modulation could reverse or stop fibrosis process and then improve intercellular communication in the myocardium. Project results should contribute to new knowledge potentially usable in clinical practice as well.
Duration: 1.1.2019 – 31.12.2022
PROTEKCIA KARDIOVASKULÁRNEHO SYSTÉMU PRI EXPERIMENTÁLNEJ HYPERTENZII A ZLYHANÍ SRDCA DUÁLNOU INHIBÍCIOU NEPRILYZÍNU A AT1 RECEPTOROV PRE ANGIOTENZÍN II: POROVNANIE S ACE-INHIBÍCIOU A MELATONÍNOM
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2019 – 31.12.2022
Účinky prírodných a syntetických zlúčenín pri oxidačnom poškodení biomakromolekúl. Pro- a antioxidačné mechanizmy.
Program: VEGA
Project leader: RNDr. Valachová Katarína PhD.
Annotation:
Duration: 1.1.2019 – 31.12.2022
Účinok bakteriálneho endotoxínu na komunikačné spojenia ciev srdca za podmienok hypertenzie.
Program: VEGA
Project leader: Ing. Frimmel Karel PhD.
Duration: 1.1.2019 – 31.12.2022
Vplyv fruktózovej diéty v experimentálnych modeloch metabolického syndrómu a u zdravých jedincov: návrh účinnej farmakologickej liečby
Effect of fructose diet in experimental models of metabolic syndrome and in healthy subjects: proposal of effective pharmacological treatment
Program: VEGA
Project leader: RNDr. Gáspárová Zdenka PhD.
Annotation: The project will contribute to the knowledge about etiopathogenesis of metabolic syndrome (MetS). It extend the current project, where we study the impact of high-fat and high-fat-high-fructose diet on hypertriacylglycerolemic(HTG) rats. Chronic diseases such as MetS are generally multifactorial. Thus in their origin and development play a role not only environment (diet, physical activity, stress) but also genetic predisposition. In the new project, we will examine effect of fructose diet (FD) on various animal models (spontaneously hypertensive, HTG, Zucker obese/nonobese and Wistar healthy rats) and find which main risk factor of MetS together with FD cause the most serious damage. We will test the effect of the prospective pyridoindole SMe1EC2 and omega-3 fatty acids on the established model. By combining of these drugs, we expect increased effect on a number of risk factors of cardiovascular and cerebrovascular diseases without causing adverse effects, thus a proposal for a new more effective treatment.
Duration: 1.1.2019 – 31.12.2022
Deriváty kyseliny 1-indoloctovej ako inhibítory aldózareduktázy: vzťah štruktúry a aktivity
Indole-1-acetic acid derivatives as aldose reductase inhibitors: structure – activity relationships
Program: VEGA
Project leader: Ing. Šoltésová Prnová Marta PhD.
Annotation:
Duration: 1.1.2018 – 31.12.2021
Elektrofyziologické koreláty a determinanty presnosti vizuálnej pracovnej pamäti
Electrophysiological correlates and determinants of visual working memory precision
Program: VEGA
Project leader: MUDr. Riečanský Igor PhD.
Duration: 1.1.2019 – 31.12.2021
Mitochondrie ako kľúčový efektor v procesoch kardioprotektívnych intervencií
Mitochondria as a key effector in processes of cardioprotective intervention
Program: VEGA
Project leader: Ing. Ferko Miroslav PhD.
Annotation:
Duration: 1.1.2018 – 31.12.2021
Nitrózo-sulfidová signálna dráha – nové regulačné vazoaktívne účinky v rôznych modeloch artériovej hypertenzie
Nitroso-sulphide signal pathway – novel regulator vasoactive effects in different types of arterial hypertension
Program: VEGA
Project leader: RNDr. Čačányiová Soňa PhD.
Annotation: Nitric oxide (NO) and hydrogen sulphide (H2S) belong to important gaseous molecules engaged to the regulation of arterial tone in normotensive conditions. NO and H2S interaction includes a formation of new products which are part of an original nitroso-sulphide signalling pathway. Our previous experiments in Wistar rats demonstrated that these new signal molecules triggered a specific vasoactive response, different from the effect evoked by NO and H2S. In condition of arterial hypertension, the vasoactive effects of the novel signalisation have not been described yet. The aim of this project is to characterise the role of NO and H2S as well as of the nitroso-sulphide signalling pathway in different animal models of hypertension: essential (SHR), NO-deficient and also metabolic syndrome (hypertriglyceridemia – HTG). A simultaneous investigation of human vessels isolated from patients with hypertension and dyslipidemia represents an appropriate way how to associate results of basic research with clin. practise.
Duration: 1.1.2018 – 31.12.2021
Nové látky pre prevenciu a terapiu ochorení spôsobených toxicitou glukózy
Novel compounds in prevention and treatment of diseases caused by glucose toxicity
Program: VEGA
Project leader: RNDr. Májeková Magdaléna PhD.
Annotation: In the long term lasting plasma glucose level may affect the course of physiological processes through numerousmetabolic pathways. A polyol pathway ranks among the important mechanisms of glucose toxicity and its mainenzyme – aldose reductase – is a frequent target in design of drugs reducing the progress of chronic diabeticcomplications. Another target could be introduced by calcium homeostasis in cells, as the cytosolic calcium levelis an important factor for many physiological processes, e.g. the insulin secretion. A modulation of calcium pumpSERCA becomes another mechanism for the manifestation of glucose toxicity. The aim of our project is to findnew compounds with polypharmacological effect, by means of combinatorial library of potential aldose reductaseinhibitors and actual knowledge on SERCA activity.
Duration: 1.1.2018 – 31.12.2021
Nové metódy prevencie a liečby oxidačného stresu, ischemicko-reperfúzne poškodenie a transplantácia srdca
Program: Other projects
Project leader: D.h.c., Prof., MUDr. Slezák Ján DrSc., FIACS
Duration: 1.11.2019 – 31.12.2021
Nové prístupy k liečbe kachexie, zápalu a oxidačného stresu v experimentálnej artritíde: Účinok rôznych rastlinných extraktov z olivových listov, Rhodiola rosea, Tribulus terrestris a extra panenského olivového oleja
Program: VEGA
Project leader: PharmDr. Poništ Silvester PhD.
Annotation:
Duration: 1.1.2019 – 31.12.2021
Ochrana srdca v situáciách nadmernej tvorby kyslikových a nitrozylových radikálov: Molekulárny vodík ako nový potenciálny therapeutický nástroj?
Heart protection in situations of excessive formation of oxygen and nitrosyl radicals: Molecular hydrogen as a new potential therapeutic tool?
Program: VEGA
Project leader: D.h.c., Prof., MUDr. Slezák Ján DrSc., FIACS
Annotation:
Project web page: https://evega.minedu.sk/e-vega/(S(yi1zctb4lxjmuf55ntk1jfbm))/default.aspx
Duration: 1.1.2018 – 31.12.2021
TraiN-SafeMDs – Školiaca sieť zameraná na zvýšenie bezpečnosti zdravotníckych pomôcok – fokus na ústnu dutinu
Training Network for improving of safety of medical devices – focus on oral cavity
Program: SRDA
Project leader: Dr.rer.nat., Ing. Kanďárová Helena ERT
Annotation: Medical devices (MDs) have irreplaceable role in modern healthcare. The term ‘medical device’ covers a broad spectrum of products that are crucial in diagnosis and treatment, disease prevention and improving the quality of life of people suffering from disabilities or injuries. MDs used in the oral cavity are usually those helping in the treatment of aphthae or canker sores irritations and lesions of the oral mucosa by forming a barrier that adheres to the oral mucosa and promotes healing. Dental materials and dental prosthetic devices are also an important group of MDs with apparent contact with oral mucosa. Most of the MDs bio-compatibility assessments is still conducted in animals. However, thanks to the advances in cell and 3D tissue engineering and due to the accelerated progress in validation of alternative methods, the MD regulations are also in vitro tests, as demonstrated recently by the adoption of the in vitro reconstructed epidermis test for intra-cutaneous testing into the ISO standard 10993-23 (Kandarova et al.,/DeJong et al., 2018). Biocompatibility testing of MDs is based on the toxicity assessment of extracts from MDs, that are in fact highly diluted solutions of potential irritants. Therefore any already validated in vitro tests and prediction models must be fine-tuned to achieve different levels of sensitivity for this specific type of materials.The proposed project builds on the practical experiences gained in the validation study for intra-cutaneous testing of MDs in which the research teams from Slovakia and Czech republic participated between 2012-2018. The current project will use 3D reconstructed tissues of oral/buccal epithelia and cell cultures with the origin in oral cavity with the aim to develop highly sensitive testing strategy for local tolerance testing in vitro. The project alsoaims into the training of PhD students and early career scientist in the use of in vitro methods for the safety assessment of MDs.
Duration: 1.3.2020 – 31.12.2021
Štúdium spúšťacích faktorov a mechanizmov prenosu signálu indukovaných neinvazívnymi adaptačnými intervenciami v organizme potkana za účelom ochrany myokardu pred schémiou
Study of triggering factors and signal transduction mechanisms induced by noninvasive adaptive interventions in rats aimed to protect myocardium against schemia
Program: VEGA
Project leader: MUDr. Ravingerová Táňa DrSc., FIACS
Annotation: Cardiovascular diseases are one of the leading causes of mortality in modern society. They are predicted to rise over the coming decades, due to aging population, longer survival of patients after myocardial infarction, and incidence of civilization diseases. Research pointed out to the protective effects of adaptive phenomenon of ischemic preconditioning (IPC) and its novel clinically acceptable and safer forms. Currently, cellular mechanisms activated by stimuli like exercise, acute hypoxia and PC of the remote organ are not yet completely elucidated as compared with classiccal IPC. For that reason, several pathological animal models (myocardial ischemia, hypertension, d. mellitus, dyslipidemia) will be used. Acute and longer lasting adaptive interventions will be tested using relevant methodology (combination of physiological, morphological and biochemical techniques). The results obtained in this project may lead to development of novel or modified therapeutic strategies to manage myocardial ischemia
Duration: 1.1.2018 – 31.12.2021
Vplyv virtuálnej reality na senzorickú reguláciu rovnováhy, fyziologické a psychologické funkcie človeka
The effect of virtual reality on the sensory regulation of balance control, physiological and psychological functions in humans
Program: VEGA
Project leader: Mgr. Hirjaková Zuzana PhD.
Annotation: Virtual reality (VR) is an environment that can be used to assess the postural and psycho-physiologicalparameters of different groups of people. The virtual environment provides an experience close to reality inlaboratory conditions or at home. The aim of the project is to gain new knowledge about the body orientation,sensory regulation of balance control and the level of psycho-physiological stimulation in the VR. The volunteerswill be introduced into the VR and at the same time their postural activity, reactions to vibratory stimulation oflower limb muscles, physiological and psychological level of arousal and stress will be recorded. We assume thatthe project results will provide the basis for designing promising methods for testing sensory balance control andpsycho-physiological reactivity to the virtual environment. The obtained results may be helpful in rehabilitation ofpatients with postural balance disorders as well as people with psychological or physiological difficulties.
Duration: 1.1.2019 – 31.12.2021
RIDD – Výskum magnetických foriem železa v rozvoji kardiovaskulárnych chorôb a porúch správania
Research of magnetic forms of iron in development of cardiovascular diseases and behavioural disorders
Program: SRDA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation: This project proposal is focused on the investigation of the role of iron in development of cardiovascular and behavioural disorders, prevalence of which is increasing during aging. The aim of this project is to investigate theimpact of aging on the metabolism of biogenic iron and its magnetic properties in association with metabolic and functional alterations in the cardiovascular system and brain in rats with various genetic predispositions tohypertension. We will determine the molecular biological changes on the level of gene expression, their encoded proteins and the activities of the enzymes involved in the endogenous antioxidant protection, the regulation of nitric oxide production and cell death due to ferroptosis in course of aging. We will also investigate the impact ofexogenously administered iron in the form of the biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) on blood pressure regulation and function of the heart and blood vessels in conditions of normotension, chronically increased blood pressure and acute stress (i.e. acutely elevated blood pressure).Results achieved in this project will contribute to better understanding of the effects of the altered iron metabolism, magnetic forms of bodily iron, as well as iron in the form of USPIONs, on the cardiovascular andcentral nervous systems and to prevention of cardiovascular risk resulting from the use of USPIONs in targeted drug delivery or as the contrast materials for new imaging methods in medicine.
Project web page: http://www.bionanoiron.sav.sk
Duration: 1.7.2017 – 30.6.2021
Interakcia metabolických faktorov a neurogénnej signalizácie pri experimentálnych modeloch depresie
Crosstalk of metabolic factors and neurogenic signaling in experimental models of depression
Program: VEGA
Project leader: RNDr. Vranková Stanislava PhD.
Annotation:
Duration: 1.1.2018 – 31.12.2020
Mechanizmy zahrnuté v endotelovej dysfunkcii indukovanej kyselinou močovou v závislosti od veku a genetickej predispozície k hypertenzii
Mechanisms involved in uric acid-induced endothelial dysfunction depending on the age and genetic predisposition to hypertension
Program: VEGA
Project leader: MUDr. RNDr. Púzserová Angelika PhD.
Annotation: Studies have shown a significant relationship between increased concentration of uric acid in the blood (hyperuricaemia) and cardiovascular diseases, including hypertension. But there is little information on the mechanisms by which uric acid can lead to end-organ damage. Hyperuricaemia combined with hypertension is associated with endothelial dysfunction. However, the mechanisms by which hyperuricaemia causes endothelial dysfunction are not clarified. This project aims to clarify the relationship of hyperuricaemia and hypertension, especially in terms of endothelial function. The aim is to bring new results highlighting the impact of elevated concentrations of uric acid on the endothelium, and to reveal the mechanisms involved in endothelial dysfunction in conductive and resistant arteries isolated from peri-pubertal and adult normotensive, prehypertensive and hypertensive rats. The results will contribute to the knowledge about pathophysiology of hyperuricaemia-induced endothelial dysfunction.
Duration: 1.1.2017 – 31.12.2020
Redoxná homeostáza, proteostáza a zápal ako potenciálne ciele pre ovplyvnenie starnutia a s ním spojených ochorení: Modulácia pomocou látok prírodného a syntetického pôvodu
Redox Homeostasis, Proteostasis and Inflammation as Potential Targets For Influencing Ageing and Age-Related Diseases: Modulation by the compounds of natural and synthetic origin
Program: VEGA
Project leader: Ing. Račková Lucia PhD.
Annotation: Ageing is a natural and inevitable phenomenon which is also the main risk factor for the serious diseases. Free-radical theory of ageing states that organisms age because cells accumulate free radical damage over time. These changes are significantly amplified by the decline in proteolytic capacity. Microglia, as the main immune effector cells of the CNS, undergo these alterations as well. This results in their chronic activation and increased risk of neurodegeneration. The aim of the project is to investigate the potential of compounds of natural and synthetic origin to up-regulate Nrf-2/Keap-1 signalling pathway (in particular, the activation of genes responsible for maintenance of redox homeostasis and proteostasis), also in view of their simultaneous down-regulation of NFkB in microglia. Subject of the project will be also investigation of postranslantional modifications in the regulatory mechanisms of proteolysis in microglial cells.
Duration: 1.1.2017 – 31.12.2020
Úloha Nrf2 signálnej dráhy v odpovediach srdcových buniek na patologické podnety
Role of Nrf2 signaling pathway in responses of cardiac cells to pathological conditions
Program: VEGA
Project leader: RNDr. Barančík Miroslav DrSc.
Duration: 1.1.2018 – 31.12.2020
Vlastnosti Na,K-ATPázy, jedného z kľúčových systémov pre udržiavanie koncentrácie sodíka v organizme, v podmienkach zaťaženia organizmu po ožiarení.
Properties of the Na,K-ATPase, representing one of the crucial systems in maintaining the sodium homeostasis in the organism, after irradiation.
Program: VEGA
Project leader: RNDr. Vrbjar Norbert CSc.
Annotation: The present project is oriented to obtain new data concerning the maintenance of intracellular homeostasis of sodium, representing one of the unavoidable factors for appropriate regulation of cellular viability, after application of radiotherapy. Using in vivo model (rat) we will investigate the influence of gamma irradiation on the cerebral and renal Na,K-ATPase which is one of the crucial systems in maintaining appropriate intracellular concentration of sodium ions. The data will contribute to elucidation of molecular background of processes involved inmaintaining the cell’s viability in kidney and in brain from the aspect of possible protection of the organism against deleterious side-effects of radiotherapy.
Duration: 1.1.2017 – 31.12.2020
Vplyv prenatálnej hypoxie na vývin jedinca a možnosti terapie jej dlhodobých dôsledkov
Program: Other projects
Project leader: PharmDr. Piešová Michaela
Duration: 1.1.2020 – 31.12.2020
Vplyv ultra malých superparamagnetických nanočastíc železa na kardiovaskulárny systém potkana v podmienkach vysokého krvného tlaku
Effect of ultrasmall superparamagnetic iron oxide nanoparticles on the cardiovascular system of rats with high blood pressure
Program: VEGA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation: This project will investigate the effect of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) on function and structure of the arterial wall and the heart in rats with high blood pressure. We will investigate if acute stress and chronic high blood pressure can facilitate the USPIONs uptake in the arterial wall and heart, tomodify cardiovascular function, including blood pressure regulation and to induce metabolic disorders, oxidative damage and alterations of the Fe2+/Fe3+ ratio in the heart and vasculature. We will investigate if L-type ofvoltage-dependent calcium channels is involved in iron uptake after USPIONs treatment. Results achieved in this project will contribute to better understanding of USPIONs effects on the cardiovascular system in conditions ofacute stress and high blood pressure as well as on prevention of cardiovascular risk resulting from the use of USPIONs in targeted drug delivery.
Duration: 1.1.2017 – 31.12.2020
Výskum možností a rozvoj SQUID magnetometrie pre vybrané aplikácie v biomedicíne a materiálovom výskume
Research on possibilities and development of SQUID magnetometry for selected applications in biomedicine and material research
Program: VEGA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation: Project has an interdisciplinary character.The aim is to show the possibilities of use of the SQUID magnetometry in study of the actual processes in medicine, biology and material research:-in analysis of the properties and magnetic characterization of the nanoparticles and nanoliquids, especiallyultra-small superparamagnetic nanoparticles based on iron oxides (USPIONs)-in investigation of the influence of the USPIONs on the function and structure of the blood vessels and heart, on development of the oxidative damage and in study of processes of the USPIONs transport through cell membranes, blood vessels and organs of rats with normal and high blood pressure-in development of the procedures and methods of quantification of the magnetic substances content in thehuman and animal cell cultures and organs- in study and development of the aluminate glasses with photoluminescence properties and other applications.
Duration: 1.1.2017 – 31.12.2020
Vzťah medzi telesnou adipozitou a funkčnými vlastnosťami artérií u potkana
Relationship between body adiposity and functional properties of arteries in rat
Program: VEGA
Project leader: Mgr. Zemančíková Anna PhD.
Annotation: High levels of body mass index are considered as an important risk factor contributing to cardiovascular impairment. However, recent studies have brought some noteworthy findings that moderately increased amount of body fat is beneficial with respect to prognosis of patients with heart and vessel diseases. The aim of this project is to analyse the relationship between the level of body adiposity/weight and cardiovascular function in terms of blood pressure regulation and functional properties of selected arteries in rat. An effort will be made to reveal how the vessels are modulated by the adjacent perivascular adipose tissue (PVAT) in healthy normotensive and in hypertensive rats after moderate and considerable increase in body adiposity induced by dietetic interventions in different ontogenetic stages. Several biometric and hemodynamic parameters will be measured, as well as some biochemical parameters in blood and tissues and in vitro reactivity of arteries in the presence of PVAT and after its removal.
Duration: 1.1.2018 – 31.12.2020