The Department of Pharmacology of Excitable Tissues

Head of Department

RNDr. Zdenka Gáspárová, PhD.

Research

Analysis of the mechanisms responsible for altered smooth muscle activity and endothelial and epithelial function, elicited by detrimental action of reactive oxygen species produced during pathological stages (e.g. ischaemia/reperfusion, diabetes mellitus, hypertension, arthritis). By applying techniques of pharmacology and biochemistry, the modulatory action of some drugs with antioxidant and scavenging properties (e.g. pyridoindols, natural products, etc.) on the ROS-induced injury of smooth muscle functions is determined and characterised.
Cardiovascular pharmacology, cell signalling mechanisms in myocardial and vascular smooth muscle cells and in compromised myocardial tisues, use of animal experimental models.
Study of the action of compounds affecting a generation and/or the action of reactive oxygen species on central nervous tissue damaged due to oxidative stress. The main aim has been pharmacological protection of the nervous system against oxidative stress occurring in some acute and chronic neurological diseases. The problem is being analyzed on receptor, biochemical, cellular, tissue, and whole animal level.

Main Focus

  • metabolic syndrome and metabolic cognitive syndrome
  • pharmacology of cardiovascular and central nervous systems
  • preclinical research and expert evaluation of new pharmacologically active drugs affecting the cardiovascular and central nervous systems
  • action of free radicals on smooth muscle tissues
  • screening of drugs acting on smooth muscles
  • effect of drugs on neuronal excitability, synaptic transmission and synaptic plasticity (long-term potentiation) of synaptic transmission in the hippocampus
  • experimental model of neurodegeneration and model of ischemia in vitro (hypoxia with hypoglycemia)
  • consultations in cardiovascular pharmacology, animal experimental models and cardiotoxicity
  • expert activity in experimental myocardial infarction, myocardial ischaemia and reperfusion
  • side effects of drugs affecting cardiovascular system

Methods

Experimental models of mesenteric ischaemia/reperfusion and streptozotocine-induced diabetes in rats. Isometric measurement of basal and pharmacologically-elicited vascular smooth muscle mechanical activity. Biochemical determination of antioxidative and diabetic status of animal organism.
Biochemical assays. Animal experimental models of myocardial infarction and experimental therapy with different drugs. Experimental model of brain ischaemia in vitro (hippocampal slices exposed to hypoxia with hypoglycaemia). Experimental model of trimethyltin-induced neurodegeneration: an early and late stage of neuronal damage. Electrophysiological analyses (neurotransmission, excitability, synaptic plasticity) of functional damage in hippocampal slices due to ischemia in vitro or neurodegeneration. Study of biochemical markers of oxidative damage in nervous tissue (determination of thiobarbituric reacting substances; detection of protein carbonyls; enzyme and metabolic intermediate assays, etc.).

People

Name / Position E-mail Phone
Bauer, Viktor Prof. MUDr., DrSc.
Senior research worker
exfabauv@savba.sk 02/59410670
Gáspárová, Zdenka RNDr., PhD.
Head of Department
exfagasp@savba.sk 02/59410676
Kaprinay, Barbara Mgr.
PhD.Student
barbara.kaprinay@savba.sk 02/32295723
Knezl, Vladimír RNDr., PhD.
Senior Research Fellow
exfaknem@savba.sk 02/59410677
Lipták, Boris Mgr.
PhD.Student
liptak.boris@savba.sk 02/59410669
Poláková, Júlia
Technician
julia.polakova@savba.sk 02/59410676
Sotníková, Ružena RNDr., CSc.
Senior Research Fellow
exfarosa@savba.sk 02/32295716
Srnová, Monika
Technician
exfamoni@savba.sk 02/59410663
Stará, Veronika Mgr.
PhD.Student
02/59410669
Vandáková, Katarína
Technician
02/59410677