The Department of Cellular Pharmacology

Head of Department

Mgr. Tomáš Perečko, PhD.

Research

The laboratory is engaged in the study of mechanisms of receptor as well as non-receptor interactions between cationic amphiphilic drugs and isolated cells at cellular and molecular levels. Especially the effect of beta-adrenoceptor blocking and antihistamine drugs, stobadine and chloroquine on blood platelets, polymorphonuclear leukocytes and on their mutual interactions is studied. In the presence of drugs, alterations in functions of tested cells (aggregability, release reaction, free radical generation, myeloperoxidase and beta-glucuronidase release) as well as in some cellular regulatory mechanisms (calcium displacement and movement, arachidonic acid metabolism, histamine and serotonin liberation) are investigated. Pharmacological effects are correlated with the structure and physico-chemical properties of drugs studied.

  • Pharmacological regulation of functions of inflammatory cells (blood phagocytes, isolated neutrophils);
  • Cellular and molecular mechanisms participating in effects of drugs, potential drugs and synthetic derivatives of substances of natural origin;
  • Identification of key targets of autoimmunity for the design of novel anti-inflammatory therapies;
  • Production of reactive oxygen species in blood and in isolated neutrophils; differentiation between oxidants produced extra- and intracellularly;
  • Neutrophil phagocytosis, apoptosis and formation of neutrophil extracellular traps (NETs);
  • Phosphorylation of protein kinase C (isoforms alpha, beta II, delta) and phosphorylation of NADPH oxidase (subunit p40phox);
  • Mobilisation of intracellular calcium in isolated neutrophils;
  • Neutrophils stimulated by a model inflammatory disease (adjuvant arthritis in rats), effectiveness of tested substances in vivo;
  • Activity of neutrophils in rheumatic patients, effect of biological therapy;

Methods

  • Luminol- and isoluminol-enhanced chemiluminescence (reactive oxygen species in blood, neutrophils and in tissues e.g. joint, spleen, blood vessels);
  • Flow cytometry (oxidative burst, phagocytic activity, apoptosis, calcium mobilisation);
  • SDS-PAGE, western transfer blotting and immunodetection with phosphospecific antibodies (phosphorylation of protein kinase C isoforms and NADPH oxidase subunits);
  • ELISA (caspase-3 and protein kinase C activity, concentration of cytokines);
  • Fluorescence microscopy (NETs formation);

People

Name / Position E-mail Phone
Jančinová, Viera PharmDr., PhD.
Senior Research Fellow
exfavega@savba.sk 02/32295728
Kudová, Jana Mgr., PhD.
Junior Research Fellow
jana.kudova@savba.sk 02/32295726
Mačičková, Tatiana RNDr., CSc.
Senior Research Fellow
exfatama@savba.sk 02/32295727
Nosáľ, Radomír prof. MUDr., DrSc.
Senior Research Fellow
exfarano@savba.sk 02/32295725
Pažoureková, Silvia Ing., PhD.
Junior Research Fellow
silvia.pazourekova@savba.sk 02/32295726
Perečko , Tomáš Mgr. , PhD.
Head of Department
tomas.perecko@savba.sk 02/32295724
Straková, Zuzana
Technician
exfapipa@savba.sk 02/32295727
Vašíček, Ondřej Mgr., PhD
Junior Research Fellow
ondrej.vasicek@bp.cz 02/32295726